简介：Brain-machineinterfaceshaveinrecentyearsallowedparalyzedpatientstocontrolroboticarms,computercursors,andexoskeletonssimplybythinkingaboutit.Theseinterfacesrequireelectrodesthataresurgicallyimplantedintoorontopofthebraintoreadelectricalsignalsfromfiringneurons.Butanov-

简介：Microsatellitesorsimplesequencerepeats（SSR）functionwidelyandlocatedependentlyingenome.However,theircharacteristicsareoftenignoredduetothelackofgenomicsequencesofmostspecies.Kelp（Saccharinajaponica）,abrownmacroalga,isextensivelyculturedinChina.Inthisstudy,thegenomeofS.japonicawassurveyedusinganIlluminasequencingplatform,anditsmicrosatelliteswerecharacterized.Thepreliminarilyassembledgenomewas469.4Mbinsize,withascaffoldN50of20529bp.Amongthe128370identifiedmicrosatellites,90671,25726and11973werefoundinintergenicregions,intronsandexons,averaging339.3,178.8and205.4microsatellitesperMb,respectively.ThesemicrosatellitesdistributedunevenlyinS.japonicagenome.Mononucleotidemotifswerethemostabundantinthegenome,whiletrinucleotideoneswerethemostprevalentinexons.Themicrosatelliteabundancedecreasedsignificantlywiththeincreaseofmotifrepeatnumbers,andthemicrosatelliteswithasmallnumberofrepeatsaccountedforahigherproportionoftheexonsthanthoseoftheintergenicregionsandintrons.C/G-richmotifsweremorecommoninexonsthaninintergenicregionsandintrons.ThesecharacteristicsofmicrosatellitesinS.japonicagenomemayassociatewiththeirfunctions,andultimatelytheiradaptationandevolution.Amongthe120140pairsofdesignedmicrosatelliteprimers,approximately75%werepredictedtobeabletoamplifyS.japonicaDNA.Thesemicrosatellitemarkerswillbeextremelyusefulforthegeneticbreedingandpopulationevolutionstudiesofkelp.