摘要
Afterpre-cultureandtreatmentofosmosis,cotyledonsofimmaturepeanut(ArachishypogaeaL.)zygoticembryosweretransformedviaparticlebombardmentwithaplasmidcontainingachimerichphgeneconferringresistancetohygromycinandachimericintron-gusgene.Selectionforhygromycinresistantcallusesandsomaticembryoswasinitiatedat10thdpost-bombardmentonmediumcontaining10-25mg/Lhygromycin.Undercontinuousselection,hygromycinresistantplantletswereregeneratedfromsomaticembryosandwererecoveredfromnearly1.6%ofthebombardedcotyledons.ThepresenceandintegrationofforeignDNAinregeneratedhygromycinresistantplantswasconfirmedbyPCR(polymerasechainreaction)fortheintron-gusgeneandbySouthernhybridizationofthehphgene.GUSenzymeactivitywasdetectedinleafletsfromtransgenicplantsbutnotfromcontrol,non-transformedplants.Theproductionoftransgenicplantsaremainlybasedonanewlyimprovedsomaticembryogenesisregenerationsystemdevelopedbyus.
出版日期
2001年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
