Simplified heavy metal staining techniques demonstrated with Fast Plant leaf tissue

在线阅读 下载PDF 导出详情
摘要 FastPlant(Brassicarapa,Cruciferae)leaftissuefixedinglutaraldehyde-acroleinandpost-fixedinos-mium,wasexaminedforresponsetoseveraleasily-preparedheavymetalstains.Leadanduranium,separatelyandincombination,gavetypicalresultsacrossthespectrumofcellorganellets.Asasinglestainfollowingosmium,bismuthproducedimagesseeminglyequivalenttoleadanduranium.Phosphotungsticacidproducedverygoodmembranedelineationbutproducedawashed-outbackgroundimagesimilartothatfromleadstaining.Carbohydratecompoundswereespeciallyresponsivetoruthenium;thecytoplasmandthematrixofallorganelleswerealsostainedverywell.Theprocedureswerenomoredemandingthantraditionalstainingmethodsandmaybeeasilyusedinresearchandteaching.FastPlantmaterialsareareliable,quickandeasysourceoflivingmaterial.
机构地区 不详
出处 《细胞研究:英文版》 1991年1期
出版日期 1991年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)
  • 相关文献