摘要
Objective:Toobservetheeffectofelectroacupuncture(EA)onsynapticstructureofhippocampalnervefeltsandsynaptophysin(SYN)expressioninratswithcerebralischernicinjury.Methods:SixtyWistarratswererandomizedintoshah-operationgroup,cerebralischemia(CI)groupandEAgroup,eachofwhichwasfurtherdividedinto1week(W)and5Wsubgroups.Clinjurymodelwasestablishedbyocclusionofthebilateralcomrnoncarotidarter-ies.“Baihui”(百会GV20),“Dazhui”(大椎GV14),“Renzhong”(人中GV26)and“Guenyuan”(关元CV4)werepuncturedandstimulatedelectrically.Thebraintissuesectionscontaininghippocampusregionwerestainedwithirnrrunohistochernicaltechniqueandobservedunderlightmicroscopeandtransmissionelectronicmicroscope.Results:AfterCI,theischemicinjuryasdegenerationofthepresynapsecompositions,decreaseofthesynapticnumeraldensity,andlowexpressionofSYNwereobservedinhippocampalCA1area.Bythe5^thweekafterCI,theneonatalsynapsesofCIandEAgroupsappeared,andSYNexpressionwasupregulated.InEAgroup,therecoveryofthenumeraldensityofsynapseswasespeciallynoticeable,being93.8%ofthatofsham-operationgroupandsignificantlyhigherthanthatinCIgroup(P<0.01).Comparedwithsham-operationgroup,thecalibratedopticaldensity(COD)valuesofSYNincreasedto70%inCIgroup,and93.3%inEAgroup,andCODvalueinEAgroupwassignificantlyhigherthanthatinCIgroup(P<0.01).Conclusion,EAcanfunctioninpromotingsynapticregenerationandenhancingandperfectingtheactionsofthereconstructedsynapsesinhippocampalCA1areainCIrats.
出版日期
2004年03月13日（中国期刊网平台首次上网日期，不代表论文的发表时间）
