摘要
【摘要】 目的 进一步研究干扰素体外释放酶联免疫检验技术在结核病诊断中的优势， 为未来进一步提高结核病检出率奠定基础。方法 138 例患者， 分别接受结核菌素皮肤过敏试验与干扰素体外释放酶联免疫检验。比较两种检查方法诊断结核病的灵敏度、特异度 ; 不同结核病患者干扰素体外释放酶联免疫检验诊断准确率。结果 138 例患者， 病理确诊结核病 42 例， 非结核病 96 例。干扰素体外释放酶联免疫检验诊断结核病的灵敏度为 85.71% （ 36/42 ）、特异度为 90.63% （ 87/96 ）， 均高于结核菌素皮肤过敏试验的 66.67% （ 28/42 ）、 80.21% （ 77/96 ）， 差异具有统计学意义（ P<0.05 ）。肺结核患者 27 例， 肺外结核患者 15 例。肺外结核患者干扰素体外释放酶联免疫检验诊断准确率为 100.00% ， 高于肺结核患者的 77.78% ， 差异具有统计学意义（ P<0.05 ）。结论 干扰素体外释放酶联免疫检验方法满足结核病临床诊断的需求， 该诊断方法具有更高的阳性检出率， 并且还具有操作步骤简单、结果精准等优点， 能够减少误诊、漏诊等情况发生， 为结核病的早期预防、早期治疗奠定基础， 因此值得在临床上做进一步推广。
【关键词】 干扰素体外释放酶联免疫检验技术 ; 结核病 ; 结核菌素皮肤过敏试验
[Abstract] objective to further study the advantages of in vitro release of interferon enzyme-linked immunosorbent assay (ELISA) in the diagnosis of tuberculosis and lay a foundation for further improving the detection rate of tuberculosis in the future. Methods 138 patients were tested by tuberculin skin allergy test and interferon releasing enzyme-linked immunosorbent assay. The sensitivity and specificity of the two methods were compared, and the diagnostic accuracy of ELISA for ifn-ifn-release in vitro in different tuberculosis patients was compared. Results of the 138 patients, 42 were confirmed by pathology and 96 were non tuberculosis. The sensitivity and specificity of ELISA were 85.71% (36 / 42) and 90.63% (87 / 96) respectively, which were higher than 66.67% (28 / 42) and 80.21% (77 / 96) of tuberculin skin allergy test. There were 27 cases of tuberculosis and 15 cases of extrapulmonary tuberculosis. The diagnostic accuracy of ELISA was 100.00%, higher than that of 77.78% (P < 0.05). Conclusion the ELISA method of interferon releasing in vitro can meet the needs of clinical diagnosis of tuberculosis. This method has higher positive detection rate, simple operation steps and accurate results, which can reduce misdiagnosis and missed diagnosis and lay a foundation for early prevention and treatment of tuberculosis, Therefore, it is worthy of further promotion in clinical practice.
出版日期
2020年09月22日（中国期刊网平台首次上网日期，不代表论文的发表时间）
