简介：PotexperimentswerecarriedouttoestimateN2fixationbyvetch,milkvetch,sicklealfalfaandbroadbeaninpurestandusinga^15N-labelledsoil.Winterwheatwasusedasthenon-fixingcontrol.The15N-labelledsoilusedwaspreparedbygrowingcorn-wheat-cornsuccessivelyonanearlyorganic-matter-freeXiashuloesssupplementedwithadequateamountsof(15NH4)2SO4,P,Kandmicronutrients,thenincorporatingthese15N-labelledplantmaterialsintothesoilaftereachhavest,andallowingtheplantmaterialstobedecomposedaerobicallyfor410dafterincorporationoftheplantmaterialofthethirecrop.The15Nenrichmentofwheatplant-Nvariedslightlywithorgans,withamaximumdifferenceof9.8%,Basedon15NenrichmentofsoilNinferredfromthemeanvalueofthe15Nenrichmentindifferentorgansofwheat79%-91%oftotalNinthetopsand67%-74%oftotalNintherootsoflegumesstudiedwerederivedfromatmosphere.EstimatebyisotopedilutionmethodwasingoodagreementwiththatbytheconventionaldifferencemethodprovidedvaluesobtainedbythelatterwerecorrectedforseedN,andalsowiththatfromthemeasurementofNaccumulatedinthetopsofthelegumes.