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简介：Diversityofbacteriawasstudiedindeep-seasedimentsfromtheShikokuBasinintheNorthwestPacificOceanbyPCR,RFLPandsequenceanalysisof16SrDNAandcomparingwithGenbankdatabase.BasedontheRFLPprofilegenerated,77clonesfromthe16SrDNAlibraryweredividedinto27types.Phylogeneticanalysisshowedthatthe27independentclonesfellintofourgroups:Proteobacteria(62.96%),Chloroflexi(14.81%),Planctomycetes(14.81%)andAcidobacteria(7.41%).Amongallsequencedclones,6wererelatedtothesulfurorsulfatemetabolismbacteriaandtheresultsalsodemonstratedthatsomebacteriaindeep-seasedimentshadrelationtomatter-energycirculation.

简介：1089strainsofyeastswereobtainedfromseawater,seasediments,mudofseasalterns,gutsofmarinefishes,mangroveplantsandmarinealgae.Theresultsofroutineidentificationandmolecularanalysismethodsshowthat44strainsamongthemarineyeastsobtainedinthisstudybelongtoCandidatropicalis,whichmayindicateitswidedistributionindifferentenvironment,especiallyinthetropicalandsubtropicalmarineenvironment.ThewidedistributionofC.tropicalisindicatesthatitmayplayanimportantroleinmarineenvironmentandthemarineenvironmentinturnisagoodsourceforobtainingC.tropicalis.

简介：Xiangshanbayisanarrowsemi-closedbayandsituatedonthenorthwesterncoastoftheEastChinaSea.Overpastdecades,ithasbecometoamajorbaywithintensivehumanactivities,denseurbanizedarea,andpoorwaterquality.Theaimofthispaperwastorevealtheecologicalstatusthroughtheelucidationofthespeciescomposition,abundance,biomassanddiversityofmacrobenthosinthisbay.SixintertidalsectionsweresurveyedfromJanuary2007toNovember2008quarterly.SectionsTG,HDandXHarelocatedinthethreeinnerbays,sectionsQJandWSarelocatednearthethermalpowerplants,andsectionXXislocatedattheouterpartofXiangshanBay.Greatvariationsinmacrobenthoscommunitywereindentified,andthespeciescompositionofthecommunityinthepresentstudyshowedthedominanceintheorderofmolluscs(bivalvesandgastropods),crustaceansandothers,andonlyfewPolychaetawererecorded.Onlythreedominantspecies,Littorinabrevicula,Ilyplaxtansuiensis,andCerithideacingulatawerecollectedinallthesections,andatotalof19dominantspecieswererecordedonlyinonesection.Two-wayANOVAanalysesofabundanceindicatedthatthereweresignificantdifferencesamongsectionsorseasons.Shannon-Wienerdiversityindex(H’)haditsmaximum(2.45)insectionQJ,andminimum(1.76)insectionTG.Multipleirregulark-dominanceplotsclearlyshowedthatthestudyareawaspollutedandthemacrobenthoscommunitywasunderstress.WeconcludethatthemacrobenthosofXiangshanBayhavebeendisturbedbyhumanactivities,especiallyattheinteriorbay.

简介：Thegeneticdiversityanddifferentiationof6mudcrabScyllaserratapopulationsfromsoutheasternChinaareinvestigatedusingallozymeelectrophoresis.Themeannumberofallelesperlocuspopulationis1.3±0.1;thepercentageofpolymorphiclociperpopulationis27.3;theobservedheterozygosityrangesfrom(0.195±0.083)to(0.241±0.090)andtheexpectedheterozygosityrangesfrom(0.105±0.043)to(0.131±0.047).Thecoefficientofgenedifferentiationamongpopulationsislow(Fst=0.032),indicatingthatonly3.2%ofthetotalgeneticdiversitycomesfrominter-population,whiletheremaining96.8%comesfromintra-populationdifferences.Thegeneticdistanceamongpopulationsis0.000～0.008(theaverageis0.002).Geneflowamongthepopulationsislarge(Nm=7.56).Geneticstructureisverysimilaramong6Scyllaserratapopulations.

简介：PCR-DGGEapproachwasusedtoanalyzebacterialdiversityinthebottomsectionofsevenarcticseaicesamplescolletedfromtheCanadaBasin.Thirty-two16SrDNAsequenceswereobtainedfromprominentDGGEbands.Theclosestrelativesofthesesequencesarefoundtobethoseofcultivatedorunculturedbacteriafromantarcticorarcticseaice.Phylogeneticanalysisclusteredthesesequencesorphylotypeswithinα-proteobacteria,γ-proteobacteriaandCFB(cytophaga-flexibacter-bacteroides)group.Sequencesbelongingtoγ-proteobacteriaweredominantandmembersoftheCFBgroupwerehighlyabundant.ItwassuggestedthattheCFBgroupwastherepresentativeofthebottomsectionofseaicesamples.

简介：Thisstudyinvestigatedthebacterialdiversityofgutcontentofseacucumber(Apostichopusjaponicus)anditshabitatsurfacesedimentinabottomenhancementareausingPCR-baseddenaturinggradientgelelectrophoresis(DGGE)technique.BacterialdiversityevaluationshowedthatthevalueoftheShannon-WienerindexofgutcontentindifferentintestinalsegmentsofA.japonicusvariedbetween2.88and3.00,lowerthanthatofthesurroundingsediment(3.23).PhylogeneticanalysisshowedthatbacterialphylotypesingutcontentandthesurroundingsedimentofA.japonicuswerecloselyrelatedtoProteobacteriaincludingγ-,α-,δ-andε-proteobacteria,Bacteroidetes,Firmicute,andActinobacteria,ofwhichγ-proteobacteriawerepredominant.TheseresultssuggestedthattheseacucumberA.japonicuswascapableoffeedingselectively,andPCR-DGGEwasapplicableforcharacterizingthebacterialcommunitycompositioningutcontentandthesurroundingsedimentofseacucumber.Furtherinvestigationtargetinglonger16SrDNAgenefragmentsand/orfunctionalgeneswasrecommendedforobtainingmoreinformationofthediversityandfunctionofbacterialcommunityinthegutcontentofseacucumber.

简介：放大碎片长度多型性(AFLP)技术被用来在马尼拉蚌(Ruditapesphilippinarum)的四张自然人口分析基因差异，在中国的四个海区域分布式，我。e.，Bohai海，Huanghai海，华东海和华南海。264AFLPloci在195individuals被分析并且揭示了基因差异的高水平。多态的loci的百分比从92.13%～96.06%并且香农“s信息索引从0.2568～0.2756。由分析分子的变化(AMOVA)，有在Qingdao和另外的三个海区域的人口之间的基因区别的高水平，这被发现。由Nei的聚合分析“spairwisedistances由地理起源组织了标本，除了Qingdao的人口。一个结论能被得出在在中国和一些不同差别的马尼拉蚌的四张自然人口有高基因的差异，这存在在之中并且在四张人口之间。结果也显示人的耕作活动将在Qingdao的人口的基因结构上有大影响。

简介：18SribosomalDNA基因(18SrDNA)序列(近似在长度的1300bp)从从与线虫大批从Qingdao海岸的内部潮汐的沉积收集的免费生活的海洋的线虫提取的DNA被放大特定的教材。PCR产品被克隆，重新放大，与Rsa消化了我和Hin6我限制endonucleases和分开的inagarose胶化。在17种限制碎片长度类型之中，打1，2和6盖住61.2%，分别地，当时，克隆的14.4%and9.3%分析了留下14仅仅盖住21克隆，它说明了15.1%总数。24代表性的克隆andphylogenetically被定序由指在RDP和GenBank数据库当前可得到的那些分析了。尽管它是难的由于已知的海洋的免费生活的线虫的18SrDNA顺序数据的缺乏把这些序列分到已知的种类或类，获得的序列被分到Adenophorea的线虫。在他们之中，12个序列接近了PontonemavulgareandAdoncholaimussp，四到Daptonemaprocerus并且二(相同)到Enoplusbrevis。我们的结果证明免费生活的海洋的线虫差异能被检索的PCR决定，18SrDNA的分析定序，仅当免费生活的海洋的线虫的18SrDNA序列被积累到某程度，一个18SrDNA序列能被分到种或一个类。

简介：ZooplanktonsurveyswerecarriedoutonNovember2-3andDecember8-10,2001at12stationsintheNanWanBayofTaiwan,China.Altogether92quantitativezooplanktonsampleswerecollectedfromsubsurfacewaterandbottomwaterlayerswithtwoconicalplanktonnets(180cminlength,45cminopeningdiameter,333μmand200μminmeshsize).Atotalof31speciesofSiphonophoreswereidentified,amongthem7speciesarenewrecordsinthewatersaroundTaiwanIsland,ofwhichRocaceacymbiformisisanewrecordinChina.DominatedbyChelophyesappendiculata,Bassiabassensis,Diphyesbojani,Diphyesdispar,AbylopsiseschscholtziandChelophyescontorta,thesespeciesaccountedforover76%and63%ofthetotalabundanceinNovemberandDecember.ThespeciesnumberandSiphonophoresabundanceinDecember(25species,1.99inds/m3)weremorethanthoseinNovember(19species,0.438inds/m3),andtheyweremoreinthesurfacewaterlayerthaninthebottomlayeratmostsamplingstations.Inearlywinter,theoffshorehighsalinitywatermasswasamainfactorinfluencingthedistribution.ThesamplingefficiencyfortwoplanktonnetsisdiscussedandtheseasonalvariationofspeciesnumberandabundanceintheNanWanBayiscomparedwiththatintheneighboringwaters.