简介：目的：利用LCMS分析技术快速鉴定单面针中的生物碱结构。方法：采用XAquaC8色谱柱（150mm×2.1mm，5μm）；以乙腈0.1%甲酸水溶液为流动相梯度洗脱；体积流量：0.3mL·min^-1；ESI源正离子模式检测；根据对照品的裂解规律和参考文献推测生物碱的结构。结果：从单面针的甲醇提取物中鉴定了20个化合物结构。结论：LCMS方法能快速、准确地鉴定单面针中的化学成分，为单面针活性成分的开发利用提供科学依据。

简介：ThefruitsofPaulowniacatalpifoliaGongTongareusedasaChinesefolkherbalmedicineforthetreatmentofenteritis,tonsillitis,bronchitis,anddysentery,etc.OurpreviousstudyhasidentifiednewC-geranylatedflavanoneswithobviousanti-proliferativeeffectsinlungcancerA549cells.Inthepresentstudy,anewC-geranylatedflavone,paucatalinoneC(1)andfiveknownC-geranylatedflavanones(2-6)wereisolated.Inaddition,atotalof34C-geranylatedflavonoidsweredetectedbyHPLC-DAD-ESI-MS/MScouplingtechniquesfromtheCH_2Cl_2extractofP.catalpifolia.Futhermore,anti-agingeffectsofisolatedcompoundswereevaluatedinvitrowithprematuresenescent2BScellsinducedbyH_2O_2.PhytochemicalresultsindicatedthatP.catalpifoliawasanaturalresourceofabundantC-geranylatedflavonoids.Diplacone(3)andpaucatalinoneA(5)werethepotentanti-agingagentsintheprematuresenescent2BScellsinducedbyH_2O_2andtheC-geranylsubstituentmaybeanimportantfactorbecauseofitslipophiliccharacter.

简介：目的：比较不同产地及生炒酸枣仁的蛋白质电泳结果，分析它们之间的共性和差异。方法：本实验采用垂直平板聚丙烯酰胺凝胶电泳（PAGE）的技术对40个不同酸枣仁样品进行分析评价。结果：根据电泳指纹图谱整理分析的数据得到对酸枣仁的初步聚类分析结果。结论：聚丙烯酰胺凝胶电泳可以大致定性区别不同产地的酸枣仁样品。

简介：目的：应用ITS2序列对朱砂根及其混伪品进行鉴定研究，为中药临床准确用药、市场规范化管理等提供依据和保障。方法：对朱砂根及其混伪品进行DNA提取、PCR扩增ITS2序列、双向测序，对序列进行比对、计算遗传距离。结果：朱砂根ITS2序列长度为217bp，种内有10个变异位点，有9种单倍型，平均G＋C含量为59.1%，除变种红凉伞外，朱砂根与其各混伪品的种间最小遗传距离均大于朱砂根种内最大遗传距离，所以利用ITS2序列可以鉴别朱砂根及其混伪品。结论：实验结果表明ITS2序列可以鉴别朱砂根及其混伪品，但对于其与变种的关系需进一步研究。

简介：目的：筛选文冠果壳的抗氧化及抑制肝癌HepG2细胞增殖活性部位。方法：用水,10%、30%、50%、70%、95%乙醇水溶液从文冠果中提取得到A、B、C、D、E和F提取物,用清除自由基DPPH·能力筛选抗氧化活性部位,用MTT法筛选抑制肝癌细胞增殖的活性部位。结果：6个提取部位均有抗氧化活性和抑制HepG2细胞增殖作用,其中E（70%乙醇水提取）部位的抗氧化活性最强,当质量浓度为0.2mg·mL-1时,其对DPPH·清除率能达到70.82%;提取部位F（95%乙醇水提取）抑制HepG2细胞增殖作用最强,当其质量浓度为75μg·mL-1时,抑制率高达70.1%。结论：文冠果壳提取物具有抗氧化及抑制人HepG2增殖活性,具有开发前景。

简介：ThesaponinginsenosideRk1isamajorcompoundisolatedfromginseng.GinsenosideRk1hasbeenreportedtohaveanti-inflammatoryandanti-tumorpropertiesandtobeinvolvedintheregulationofmetabolism.However,theeffectandmechanismofanti-inflammatoryactionofginsenosideRk1hasnotbeenfullyclarified.WeinvestigatedwhetherginsenosideRk1couldsuppresstheinflammatoryresponseinlipopolysaccharide-stimulatedRAW264.7macrophagesandtoexploreitsmechanismoftheaction.RAW264.7cellsweretreatedwithLPS(1μg×mL~(–1))intheabsenceorthepresenceofGinsenosideRk1(10,20,and40μmol×L~(–1)).ThentheinflammatoryfactorsweretestedwithGriessreagents,ELISA,andRT-PCR.TheproteinswereanalyzedbyWesternblotting.GinsenosideRk1inhibitedlipopolysaccharide-inducedexpressionofnitricoxide(NO),interleukin(IL)-6,IL-1β,tumornecrosisfactor(TNF)-α,andmonocytechemotacticprotein(MCP)-1.GinsenosideRk1inhibitedthelipopolysaccharide-stimulatedphosphorylationofNF-κBandjanuskinase(Jak)2andsignaltransducerandactivatoroftranscription(Stat)3atSer727andTyr705.ThesedatasuggestedthatginsenosideRk1couldinhibitexpressionofinflammatorymediatorsandsuppressinflammationfurtherbyblockingactivationofNF-κBandtheJak2/Stat3pathwayinLPS-stimulatedRAW264.7cells.

简介：Thepresentstudywasdesignedtosynthesize2-Cyano-3,12-dioxooleana-1,9(11)-en-28-oate-13β,28-olide(1),alactonederivativeofoleanolicacid(OA)andevaluateitsanti-inflammatoryactivity.Compound1significantlydiminishednitricoxide(NO)productionanddown-regulatedthemRNAexpressionofiNOS,COX-2,IL-6,IL-1β,andTNF-αinlipopolysaccharide(LPS)-stimulatedRAW264.7cells.FurtherinvivostudiesinmurinemodelofLPS-inducedacutelunginjury(ALI)showedthat1possessedmorepotentprotectiveeffectsthanthewell-knownanti-inflammatorydrugdexamethasonebyinhibitingmyeloperoxidase(MPO)activity,reducingtotalcellsandneutrophils,andsuppressinginflammatorycytokinesexpression,andthusamelioratingthehistopathologicalconditionsoftheinjuredlungtissue.Inconclusion,compound1couldbedevelopedasapromisinganti-inflammatoryagentforinterventionofLPS-inducedALI.