简介：Objective:Toexplorethecontentchangeofneurofilament(NF)proteinsubunitsintheexperimentalbraindiffuseaxonalinjury(DAI)bylateralheadrotation.Methods:Twenty-fourSpragueDawley(SD)ratswereequallydividedintothreeinjurygroups(2h,12h,and24hpostinjury)andonecontrolgroup.ThemodelsofDAIweremadeintheinjurygroupsbylateralheadrotation.westernblottingtechniquewasusedtomeasuretheconteneofNF68(akindofNFproteinsubunit)inthebrainstemtissuesamongalltheinjuredandcontrolrats.TheNF68immunohistochemicalstainingwasusedinanothersixSDratsinordertoobservethemorphologicalchangesinDAI.Results:TheNF68contentinthebrainstemtendedtodecreaseat2hpostinjury,decreasedsignificantlyat12handcontinueditsdecreaseat24h.NF56andNF52,asthebreakdownproductsofNF68,hadatendencytoincreaseat2-12haftertheinjury,andamountedtoasignificantlyhigherlevelat24h.Microscopically,therewerealotofswellingneuronalaxonsintheventralpartofthemedullaroblongateat2haftertheinjury.someaxonsweredisconnected,andaxonalretractionballsformedontheirproximalend.Conclusions:ThereisandoccurrenceofphosphorolysiswithinthebrainsteminDAIbylateralheadrotation.ThesereactionscausethebreakdownofNF68,whichresultsinthedecreaseofNF68incontent.ItsuggeststhatthebreakdownofneurofilamentproteinsubunitsisanimportantreasonforstructuraldestroyofneurofilamentsinDAI.

简介：FromApril1995toOctober1999,135patientswithconcussionofbrainwereexaminedwiththreedimensionaltranscranialDoppler(3D-TCD)andthechangeofcerebralbloodflowinvariousphaseswasanalyzedtoevaluatetheeffectof3D-TCDondiagnosisandtherapyofbrainconcussion.

简介：Objective:ToanalyzethedynamicchangeofserumproteinS100binpatientswithtraumaticbraininjuryanditsclinicalvalueinassessingbraindamage.Methods:AccordingtoGlasgowcomascale(GCS),102casesoftraumaticbraininjuryweredividedintomildbraininjurygroup(GCS≥13,n=31,GroupA),moderatebraininjurygroup(8