简介:AIM:Todeterminetheproliferativepotentialandthemaintenanceofstemcellactivityinstoredhumanlimbaltissues,andcorrelatethiswiththepreservationtime,cellviabilityandtheexpressionofstemcellmarkers.METHODS:Thirtylimbalrimsweresplitinto4partsandstoredincornealpreservationmediumat4℃for0,1,4,or7days.ThelimbalstemcellandmitoticmarkersP63,CK19,proliferatingcellnuclearantigen(PCNA),andKi67weredeterminedbyimmunohistochemicalstaining.Theproliferativepotentialoflimbalepithelialcellswasassessedbycellviability,theabilityofgeneratingstratifiedepithelium,andcolonyformingassay.RESULTS:Thestoredtissuesmaintainedlimbalstratifiedstructureto7daysandexhibitedcomparableexpressionlevelofstemcellandmitoticmarkers.Theproportionofviablecellsdecreasedwiththeprolongedpreservationtime,whilecolonyformingefficiencydecreasedfromthe1stdayanddisappearedatthe4thday.Wheninoculatedonamnioticmembrane,thecellspreservedfor1dayformedastratifiedepithelium,whilethecellsfrom4days’preservationformedadiscontinuouslayer.CONCLUSION:Thecolonyformingefficiencyoflimbalepithelialstem/progenitorcellsdecreasedrapidlywiththeincreasingpreservationtime,whiletheexpressionlevelofmarkersandcapacityofformingepithelialmonolayeronamnioticmembranedecreasedgradually.Thelimbalepithelialstemcellslosttheirfunctionearlierthanthelostexpressionlevelofstemcellmarkers.Thismayhelpustobetterchoosetheappropriatepreservationgraftsforfuturelimbalstemcelltransplantation.
简介:目的探讨全视网膜光凝治疗缺血型视网膜中央静脉阻塞的临床效果。方法回顾性分析2003年1月至2005年4月我院收治的缺血型视网膜中央静脉阻塞共68例68眼,其中行532nm激光全视网膜光凝治疗40眼(激光组),28眼末行532nm激光全视网膜光凝(对照组),观察对比两组初、末诊视力,新生血管及新生血管性青光眼等并发症情况。随访时间均在8个月以上,平均随访11±2.3个月。结果两组末次随访视力、虹膜新生血管及新生血管性青光眼等并发症发生率有所不同,但比较差异无统计学意义(P〉0.05)。结论缺血性CRVO严重损害患者视力,需要积极治疗,全视网膜光凝对于对于预防虹膜新生血管及新生血管性青光眼的疗效不确切。
简介:目的研究高眼压及其持续时间对大鼠视网膜caspase-3表达的影响。方法通过前房灌注平衡盐液建立大鼠急性高眼压模型:高眼压持续时间均为4h,依据眼内压的不同将sD大鼠60只随机分为正常对照组、40mmHg(1mmHg=0.133kPa)组、60mmHg组、80mmHg组、100mmHg组,每组12只。将眼内压为80mmHg的48只sD大鼠随机分为正常对照组、2h组、4h组、8h组,每组12只。正常对照组不给予前房灌注,其他各组分别给予不同的高眼压或高眼压持续不同时间。各组灌注结束后快速摘除眼球,分别采用Western印迹法和免疫组织化学法检测视网膜caspase-3的表达。结果与对照组相比,40mmHg组大鼠视网膜caspase-3的表达无增加(P〉0.05);60mmHg组、80mmHg组和100mmHg组大鼠视网膜caspase-3的表达均强于正常对照组(q值分别为4.87、5.28和6.71;P〈0.01),但各组之间差异无统计学意义(P〉0.05)。与对照组相比,2h组大鼠视网膜caspase-3的表达差异无统计学意义(P〉0.05),4h组和8h组大鼠视网膜caspase-3的表达均增加(q值分别为2.81和3.67;P〈0.01)。表达caspase-3的视网膜细胞主要位于神经节细胞层、外丛状层和内丛状层。结论采用前房灌注平衡盐液制作急性高眼压模型研究高眼压大鼠视网膜caspase-3的表达时,眼内压为80mmHg、高眼压持续4h即可。
简介:目的在视网膜缺血再灌注模型上,观察再灌注后视网膜内caspase-3的动态变化,探讨caspase-3与视网膜细胞凋亡的关系。方法结扎大鼠左侧颈总动脉1h,然后再灌注,检测再灌注后1、6、12、24、48、72h大鼠视网膜内caspase-3的水平及视网膜细胞凋亡平均发生率。结果再灌注后视网膜内caspase-3的表达出现在光感受器细胞层,在再灌注后1、6、12、24、48、72hcaspase-3平均光密度分别为0.067±0.004、0.923±0.045、1.962±0.377、3.793±0.860、2.039±0.427、1.332±0.109,细胞凋亡平均发生率(%)分别为1.8±0.1,7.1±0.2,18.2±1.4,34.7±2.1,22.6±0.9,16.3±0.4。结论再灌注后大鼠视网膜caspase-3表达与细胞凋亡呈现正相关,caspase-3可以促进视网膜细胞凋亡的发生。
简介:AIM:Tocharacterizetemporalpatternofresolutionandrecurrenceofnaivechoroidalneovascularization(CNV)secondarytowetage-relatedmaculardegeneration(AMD)treatedwithintravitrealbevacizumabonasneededregimen,andtoanalyzebaselineriskfactorsforCNVresolutionorrecurrence.METHODS:Ninety-oneeyesof80patientswithnewlydiagnosedwetAMDwereretrospectivelystudied.Alleyesweretreatedwitharoundofthreemonthlyintravitrealbevacizumabinjections,followedbyoneadditional’bonus’injectionafterresolutionofCNVactivity.Duringfollow-up,eyesweremonitoredwithfluoresceinangiography,opticalcoherencetomography,andbest-correctedvisualacuity(BCVA).IncaseofrecurrencesofCNVactivity,eyeswereretreatedwithotherroundsofbevacizumabinjectionsfollowingthesametreatmentprotocol.RESULTS:Overamedianfollow-upof532d,themedianresolutiontimeofCNVactivityinthefirst,second,andthirdtreatmentroundwas98d,126d,and111d,respectively.Themedianrecurrencetimeforthethreeroundswas154d,126d,and151d,respectively.Nosignificantdifferenceinresolutiontime(P=0.09)orinrecurrencetime(P=0.11)wasdetectedamongtreatmentrounds.Age(P=0.0082)andlensstatus(P=0.035)werefoundtobeassociatedwithCNVresolution;forevery1-yearincreaseinagetherewas4%greaterchanceofCNVresolution;Phakiceyesdemonstrateda33%betterchancetoexperienceCNVresolutionthanpseudophakiceyes.ForCNVrecurrence,lensstatus(P=0.0009)andgender(P=0.0446)werefoundtobepredictive;pseudophakiceyeshada3.69-foldgreaterrisktoexperiencerecurrenceofCNVactivitycomparedtophakiceyes;maleshada2.19-foldgreaterrisktoexperiencerecurrenceofCNVactivitythanfemales.NosignificantBCVAchangesamongthreetreatmentroundswerenoted(P=0.56).CONCLUSION:ResolutiontimeandrecurrencetimeofCNVactivitywerenotsignificantlydifferentamongtreatmentrounds,suggestingabsenceoftachyphylaxistobevacizumab.Acautiousdecisionshouldbemadeupondisco
简介:目的观察球后注射山莨菪碱联合高压氧治疗非动脉炎性前部缺血性视神经病变的疗效。方法选取2015年6月-2016年6月我院收治的60例非动脉炎性前部缺血性视神经病变患者,随机分为2组:治疗组和对照组,2组均给予皮质激素、血管扩张剂、营养神经类药物等治疗。治疗组在此基础上给予山莨菪碱球后注射联合高压氧,15天后除了停止球后注射山莨菪碱,其余治疗不变,30天后比较2组治疗前后视力、视野的改变。结果视力治疗组治愈13眼,显效4眼,有效10眼,无效3眼。对照组治愈6眼,显效12眼,有效8眼,无效4眼。两组对比经ridit分析Z=-3.074,P=0.002。视野治疗组治愈15眼,显效9眼,有效3眼,无效3眼。对照组治愈3眼,显效8眼,有效14眼,无效5眼,两组经ridit分析Z=-3.504,P=0.000。结论球后注射山莨菪碱联合高压氧治疗缺血性视神经病变疗效较好。