简介：Theunsteadyhydrodynamicsofabiomimeticfinattachedtoacylindricalbodyhasbeenstudiednumericallyusingacomputationalfluiddynamic(CFD)simulatorbasedonanin-housesolveroftheNavier-Stokesequations,combinedwitharecentlydevelopedmulti-block,oversetgridmethod.Thefin-bodyCFDmodelisbasedonamechanicalpectoralfindevice,whichconsistsofacylindricalbodyandanasymmetricfinandcanmimicflapping,rowingandfeatheringmotionsofthepectoralfinsinfishes.Firstthemulti-block,oversetgridmethodincorporatedintotheNSsolverwasverifiedthroughanextensivestudyofunsteadyflowspastasinglefinundergoingrowingandfeatheringmotion.Thenunsteadyflowspastthebiomimeticfin-bodymodelundergoingthesamemotionswerecomputedandcomparedwiththemeasurementsofforcesofthemechanicalpectoralfin,whichshowsgoodagreementinbothtime-varyingandtime-averagedhydrodynamicforces.Therelationshipbetweenforcegenerationandvortexdynamicspointstotheimportanceofthematchinfinkinematicsbetweenpowerandrecoverystrokesandimpliesthatanoptimalselectionofparametersofphaselagsbetweenandamplitudesofrowingandfeatheringmotionscanimprovetheperformanceoflabriformpropulsionintermsofeithermaximumforcegenerationorminimummechanicalpower.

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简介：Thepaperpresentsamulti-scalemodellingapproachforsimulatingmacromoleculesinfluidflows.Macromoleculetransportatlownumberdensitiesisfrequentlyencounteredinbiomedicaldevices,suchasseparators,detectionandanalysissystems.Accuratemodellingofthisprocessischallengingduetothewiderangeofphysicalscalesinvolved.Thecontinuumapproachisnotvalidforlowsoluteconcentrations,butthelargetimescalesofthefluidflowmakepurelymolecularsimulationsprohibitivelyexpensive.Apromisingmulti-scalemodellingstrategyisprovidedbythemeta-modellingapproachconsideredinthispaper.Meta-modelsarebasedonthecoupledsolutionoffluidflowequationsandequationsofmotionforasimplifiedmechanicalmodelofmacromolecules.Theapproachenablessimulationofindividualmacromoleculesatmacroscopictimescales.Meta-modelsoftenrelyonparticle-correctoralgorithms,whichimposelengthconstraintsonthemechanicalmodel.Lackofrobustnessoftheparticle-correctoralgorithmemployedcanleadtoslowconvergenceandnumericalinstability.AnewFAstLinearCOrrector(FALCO)algorithmisintroducedinthispaper,whichsignificantlyimprovescomputationalefficiencyincomparisonwiththewidelyusedSHAKEalgorithm.ValidationofthenewparticlecorrectoragainstasimpleanalyticsolutionisperformedandimprovedconvergenceisdemonstratedforssDNAmotioninalid-drivenmicro-cavity.

简介：Oneofmanyinterestingresearchactivitiesinbiofluidmechanicsisdedicatedtoinvestigationsoflocomotioninwater.Someofpropulsionmechanismsobservedintheunderwaterworldareusedinthedevelopmentprocessofunderwaterauto-nomicvehicles(AUV).Inordertocharacteriseseveralsolutionsaccordingtotheirmanoeuvrability,influenceonthesur-roundingfluidandenergeticefficiency,adetailedanalysisoffin-likemovementisindispensable.Inthecurrentpaperananalysisofundulatory,oscillatoryandcombinedfin-likemovementsbymeansofnumericalsimulationiscarriedout.TheconservationequationofmassandtheconservationequationofmomentumaresolvedwiththeFiniteVolumeMethod(FVM)byuseofthesoftwareCFX-10.0.Theundulatoryandoscillatoryfinmovementsaremodelledwithanequationthatisimplementedwithinanadditionalsubroutineandjoinedwiththemainsolver.Numericalsimulationsarecarriedoutinthecomputationaldomain,inwhichonefinisfixedinaflow-throughwaterduct.SimulationsarecarriedoutintherangeoftheRenumberupto105.Theresultsshowsignificantinfluenceofappliedfinmotiononthevelocitydistributioninthesurroundingfluid.

简介：TheE(envelope)proteinisthesmalleststructuralproteininallcoronavirusesandistheonlyviralstructuralproteininwhichnovariationhasbeendetected.WeconductedgenomesequencingandphylogeneticanalysesofSARS-CoV.Basedongenomesequencing,wepredictedtheEproteinisatransmembrane(TM)pro-teincharacterizedbyaTMregionwithstronghydrophobicityandα-helixcon-formation.Weidentifiedasegment(NH2-_L-Cys-A-Y-Cys-Cys-N_-COOH)inthecarboxyl-terminalregionoftheEproteinthatappearstoformthreedisulfidebondswithanothersegmentofcorrespondingcysteinesinthecarboxyl-terminusoftheS(spike)protein.ThesebondspointtoapossiblestructuralassociationbetweentheEandSproteins.OurphylogeneticanalysesoftheEproteinsequencesinallpub-lishedcoronavirusesplaceSARS-CoVinanindependentgroupinCoronaviridaeandsuggestanon-humananimalorigin.

简介：Inthearticle,theauthorreportsonacasestudyofchronicpainproblemscausedbyherownfootdeformity.TheauthorbeganusingTaiji,ChenstyleChanSiGong(ReelingSilk),andZhanZhuang(standingmeditation)torelievechronicpainsince1986.AftermorethantwoyearsofQigongtraining,thestructureoftheauthor'sfootchangedfromthepreviousdeformitytonormalfootshape.Thesechangesenabledtheauthortowalknormallyandtobefreefrompersistentchronicpain.ThearticlediscussedthemethodsofusingQicurrentsandotherissuesthatneedtobepaidattentiontowhenpracticingTaichi,suchashowtoadjustone'smentalfocusandmaintainastateofrelaxationto"mindful".Taichiinvolvesanumberofinternal"micro-adjusting"abouthowthesetechniquesrelaxandstrengthenmusclestoreducechronicpain.ThereportanddiscussiononthetreatmentprincipleofQigongcanprovidenewstrategiesforthetreatmentofchronicpain.

简介：竞争性寡聚脱氧核糖核酸识别转录因子的DNA结合域,抑制了转录因子对基因的转录调控,转录因子E2F作为潜在的治疗靶点,可以用于抑制肾小球系膜细胞和血管内皮细胞的增生,在部分异常增生的疾病中显示了一定的应用前景.

简介：Overthelastdecade,computationalmethodshavebeenintensivelyappliedtoavarietyofscientificresearchesandengi-neeringdesigns.Althoughthecomputationalfluiddynamics(CFD)methodhasplayedadominantroleinstudyingandsimu-latingtransportphenomenainvolvingfluidflowandheatandmasstransfers,inrecentyears,othernumericalmethodsforthesimulationsatmeso-andmicro-scaleshavealsobeenactivelyappliedtosolvethephysicsofcomplexflowandfluid-interfaceinteractions.Thispaperpresentsareviewofrecentadvancesinmulti-scalecomputationalsimulationofbiomimeticsrelatedfluidflowproblems.Thestate-of-the-artnumericaltechniques,suchaslatticeBoltzmannmethod(LBM),moleculardynamics(MD),andconventionalCFD,appliedtodifferentproblemssuchasfishflow,electro-osmosiseffectofearthwormmotion,andself-cleaninghydrophobicsurface,andthenumericalapproachesareintroduced.Thenewchallengingofmodellingbiomi-meticsproblemsindevelopingthephysicalconditionsofself-cleanhydrophobicsurfacesisdiscussed.

简介：利用构建的犬2型腺病毒E3区缺失质粒pBE3L分别构建了含有和不含外源性启动子的绿色荧光蛋白(GFP)基因和狂犬病病毒糖蛋白(Rgp)基因的重组表达质粒pBE3LGFP、pBE3LCGFP、pBE3LRgp和pBE3LCRgp,并分别对DK细胞进行了转染实验,以检测其表达,结果显示,pBE3LCGFP质粒在转染DK细胞后,于36h即可观察到荧光,72～96h无明显差别,传3代后仍可见表达荧光的细胞,pBE3LCRgp质粒转染DK细胞后,于48～96h用间接免疫荧光染色可检测到糖蛋白的表达,而pBE3LGFP和pBE3Rgp质粒转染DK细胞后经检测均无表达,表明构建的E3区缺失性载体不能利用E3区自身的启动子进行目的基因的表达,但利用外源性启动子可使外源基因获得良好表达.

简介：目的：构建4E—BPl及其T37A、T46A、$65A、T70A突变体4E—BPl-4A基因表达的重组慢病毒载体，研究其对胃癌HGC27细胞生长的影响。方法：PCR扩增4E—BPl基因及其突变体aE—BPl-4A基因并克隆到pCDH载体，构建成pCDH-4E—BPl、pCDit一4E—BPl—4A，将其-9包装载体共转染293T细胞，包装成Lenti-4E—BPl及Lenti-4E—BPl—4A重组慢病毒载体，将此慢病毒感染胃癌HGC27细胞，Western印迹鉴定病毒载体介导的4E—BPl、4E～BPl—4A蛋白的表达，MTT、克隆形成和软琼脂方法研究过量表达4E—BPl、4E—BPl—4A对胃癌HGC27细胞生长的影响。结果：包装成Lenti-4E—BPl及Lenti-4E—BPl—4A重组慢病毒载体，并将此慢病毒载体感染胃癌HGC27细胞；MTT、克隆形成、软琼脂实验表明过量表达4E—BP!可抑制胃癌HGC27细胞的生长，过量表达4E—BP!一4A时抑制效果更明显。结论：构建了4E—BPl、4E—BPI-4A的重组慢病毒表达载体，在胃癌HGC27细胞中过量表达4E—BPl可抑制细胞生长，过量表达4E—BPl-4A的抑制效果更明显。

简介：Chip-basedflow-throughPCRimplementsthePCRasacontinuousprocessfornucleicacidanalytics.Thesampleistransportedinawindingchannelthroughtemperaturezonesrequiredfordenaturation,annealingandextension.Mainfieldsofapplicationarethemonitoringofcontinuousprocessesforrapididentificationofcontaminantsandqualitycontrolaswellashighthroughputscreeningofcellsormicroorganisms.Amodulararrangementwithfiveheatingzonesforflow-throughPCRisdiscussedandevaluated.Thespecialheaterarrangementallowstheimplementationofupto40cyclesonthefootprintofamicroscopeslide,whichisplacedontopofa5zonesheatingplate.Liquid/liquidtwophaseflowofPCRreactionmixtureandmineraloilhavebeenappliedtocreateasegmentedflowprocessscheme.Inthatway,thedevelopedsystemmayprovideflow-throughPCRasaunitoperationforthedropletbasedmicrofluidicsplatform.ThesingleuseofdisposabledevicesiscommonlypreferredduetothesensitivityofthePCRprocesstocontaminations.All-glassmicrofluidicchipsanddisposablechipdevices,madefrompolycarbonateasareplicationwithidenticallygeometry,havebeenfabricatedandtested.Forthefirsttime,microchannelgeometrieswithnearlycircularprofiledevelopedbyall-glasstechnologyhavebeentransferredtomassfabricationbyinjectioncompressionmolding.Bothdeviceshavebeensuccessfullyappliedforthedetectionofthetumorsuppressorgenep53.Althoughproductyieldandselectivityoftheamplificationprocessdonotdependonthechipmaterial,awelldefined,reliablesegmentedflowregimecouldonlyberealizedintheall-glasschip.

简介：目的研究二氢卟吩e6（Ce6）在移植瘤小鼠体内吸收、分布及代谢的动态变化,以期为声动力疗法处理不同部位肿瘤的时间点提供科学依据。方法艾氏移植瘤小鼠尾静脉注射Ce6后,利用荧光分光光度法和小动物活体成像技术测定Ce6在小鼠不同组织的富集分布变化规律。结果小鼠尾静脉给药后,Ce6迅速分布于全身各组织,在2h内,各组织药物浓度均达到峰值,其中以肝含量最高。随后各组织中药物浓度均开始下降,以肝中清除速度最快。肿瘤组织中的Ce6含量在注射后不断上升,2h时达到最高,随后开始下降,2～10h代谢比较缓慢,24h时浓度降至最低,但仍高于其他组织。结论Ce6在艾氏移植瘤中具有肿瘤组织选择性好、潴留时间长并可迅速从体内排出等优点,有着很好的临床应用前景,同时提出了不同组织类型不同部位的肿瘤应根据各自适当的时间点进行处理。

简介：目的：构建蜱传脑炎病毒（TBEV）结构蛋白E的原核表达载体，表达重组蛋白。方法：PCR扩增E蛋白全长基因片段，插入原核表达载体pET-32a并转化大肠杆菌进行诱导表达，镍柱纯化、复性。结果：E蛋白在大肠杆菌中获得表达，表达量达60mg／L；重组E蛋白能与人抗TBEV多克隆抗体产生特异反应；用重组E抗原免疫家兔后，能检测到较高的抗体水平。结论：原核表达的E抗原蛋白具有抗体结合活性，可用于制备ELISA诊断试剂。

简介：目的利用昆虫细胞，杆状病毒系统表达猪瘟病毒（CSFV）E2蛋白，用于E2蛋白功能、开发CSF新型疫苗以及建立相关血清学诊断方法等研究。方法采用RT—PCR扩增CSFVE2基因，将PCR产物克隆到pGEM—T—Easy载体，将该基因插入到pFast—BacHTA载体中，构建重组转座载体后转化DH10Bac感受态细胞，获得重组Bacmid质粒后转染sf9昆虫细胞，传毒3代，对表达蛋白进行Western-blot及免疫组化鉴定。结果成功克隆CSFVE2基因，其核苷酸序列为1119bp。SDS-PAGE电泳结果显示表达E2蛋白相对分子质量约为43×10^3，Western-blot和免疫组化结果证实表达蛋白能够被CSFV标准阳性血清识别。结论在Bac-to-Bac杆状病毒系统中的成功表达了CSFVE2蛋白，与CSFV标准阳性血清具有较好的反应性。

简介：维生素E是人和动物必需维生素.本文以20份向日葵种质资源为实验材料,通过对维生素E含量、含油率、皮壳率以及百粒重的测定及统计分析,试图了解向日葵种质资源中维生素E含量变异及相关数据.结果表明,在20份向日葵种质资源中,油葵的维生素E含量和含油率明显高于食葵;含油率与维生素E含量在0.01水平呈极显著正相关,含油率与百粒重在0.05水平呈显著负相关;百粒重与皮壳率在0.01水平呈极显著负相关.通过初步评价,发现3份富含天然维生素E的向日葵种质资源.

简介：以色列Technion以色列技术研究所的AndrewPLevy及其同事在ArteriosclerThrombVascBiol上报告，补充维生素E可减少中年2型糖尿病患者的心血管事件和结合珠蛋白（Hp）2-2基因型。

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简介：目的：探讨血流变学和血清学指标在骨折延迟愈合患者中的变化及其临床意义.方法：随机选取2010年1月～2016年6月在我院进行手术治疗的骨折延迟愈合及骨折正常愈合患者各90例,分别为观察组与对照组,对比分析两组患者术后第1、8、12周时血清可溶性血管细胞黏附分子1（sVCAM-1）、胰岛素样生长因子l（IGF-1）、血小板衍生生长因子（PDGF）及人可溶性细胞间黏附分子1（sICAM-1）和红细胞刚性指数、红细胞聚集指数、血浆黏度的差异.结果：术后第1、8、12周两组血清学及血流变学各指标整体相比差异均具有统计学意义（均P＜0.05）,且组内两两比较均具有统计学差异（均P＜0.05）.术后8、12周观察组血清slCAM-1、sVCAM-1、红细胞刚性指数、红细胞聚集指数、血浆黏度均高于对照组,而血清PDGF、IGF-1均低于对照组,比较差异均具有统计学意义（均P＜0.05）.结论：骨折患者血清sICAM-1、PDGF、IGF-1、sVCAM-1及红细胞刚性指数、红细胞聚集指数、血浆黏度水平会随着病程进展发生变化,并且血清sICAM-1、sVCAM-1及红细胞刚性指数、红细胞聚集指数、血浆黏度水平的升高,血清PDGF、IGF-1水平的降低可能是引起骨折延迟愈合的重要因素,对于骨折患者的临床治疗具有重要临床意义.

简介：LRP16以前在乳癌房间作为导致雌激素的基因被识别。到在子宫内膜的癌症(EC)的雌激素和它的功能的效果的LRP16的应答的海角房间仍然是不清楚的。这里，我们证明LRP16基因的信使rna水平和倡导者活动被17beta-estradiol(E2)显著地在雌激素受体高山增加哈(嗯高山哈)-positiveIshikawa人EC房间。尽管Ishikawa细胞的生长率没被LRP16的宫外的表示显然影响，Transwell试金的结果显示出LRP16-overexpressing细胞的侵略能力的近似one-thirdincrease。由于分子的屏蔽，我们观察到E-cadherin的表示，与肿瘤转移联系的一个必要粘附分子，被LRP16镇压。进一步的倡导者分析以一种剂量依赖者方式表明了那LRP16inhibitedE-cadherintransactivation。然而，抑制被雌激素剥夺废除，显示由LRP16requires的E-cadherin抄写的down规定嗯高山哈调停。染色质免疫降水分析表明到E-cadherin倡导者的ERalpha的绑定被LRP16反对，建议那LRP16能防碍嗯调停alpha的抄写。这些结果建议起来由雌激素的LRP16的规定能被在人的EC调整E-cadherin的down涉及侵略生长。