简介：目的探讨白内障防盲手术中透明质酸酶和亚甲蓝染色对麻醉效果和连续环形撕囊成功率及对手术效果的影响。方法100例（100眼）白色白内障随机分为2组进行前瞻性研究。球周麻醉中加入透明质酸酶及改良亚甲蓝前囊膜染色组53例,对照组（球周麻醉中不加入透明质酸酶和前囊不染色）47例。球周麻醉后,行现代白内障囊外摘除及人晶状体植入术。比较环形撕囊成功率、术中并发症、术后反应情况、视力、眼压。结果2组术中撕囊成功率及人工晶状体体囊袋内植入率均有差别,与对照组相比差异有统计学意义;2组术后反应情况、视力、眼压无统计学意义。结论球周麻醉中加入透明质酸酶及改良亚甲监染色明显提高连续环形撕囊成功率。降低白内障手术并发症,无明显副作用,且廉价易得,适合在防盲白内障手术中推广。

简介：AIM:Toexploretheeffectofsaturatedhydrogensalineonbluelight-inducedretinaldamageinrats.·METHODS:Theretinaldamageofratswasinducedbybluelightexposurefor6hoursandexamined8hours,16hoursand24hoursaftertheexposure.OnehundredfemaleSprague-Dawleyratswererandomlydividedintofourgroups.Group1included30ratsreceivedlightexposurewithoutanyothertreatment.Group2included30ratsreceivedlightexposurewithintraperitonealinjectionofnormalsaline.Group3included30ratsreceivedlightexposurewithintraperitonealinjectionofsaturatedhydrogensaline.AndGroup4includedtheother10ratswhichdidnotreceiveanytreatment.Theamountofintraperitonealinjectionofsaturatedhydrogensalineandnormalsalinewascalculatedintheratioof1ml/100gofratweight.SpecimenswerecollectedandprocessedbyH-Estaining,ultrastructureobservation,biochemicalmeasurement.Morphologicalchangeswereobservedbylightmicroscopeandtransmissionelectronmicroscope(TEM)andtheretinalouternuclearlayer(ONL)thicknesswasmeasuredbyIPP6.0,whilethemalondialdehyde(MDA)wasmeasuredbycolorimetricdeterminationat532nm.·RESULTS:AlthoughthestructureofretinainGroup1andGroup2wasinjuredheavily,theinjuryinGroup3wasmild.ThedifferencesbetweenGroup1andGroup2werenotsignificant.ComparedwiththeratsinGroup1andGroup2,theonesinGroup3hadmoreclearlydemarcatedretinastructureandmoreorderedcellsbylightmicroscopeandTEMobservation.TheONLthicknesses(400times)offourgroupsateachtimepointexceptbetweenGroup1andGroup2weresignificantlydifferent(P<0.05).ThethicknessesoftheONLinGroup1atthreetimepointswere30.41±4.04μm,26.11±2.82μmand20.63±1.06μm,inGroup2were31.62±4.54μm,25.08±3.63μmand19.07±3.86μm,inGroup3were29.75±3.62μm,28.83±1.97μmand27.61±1.83μm.InGroup4themeanofthethicknesswas37.35±1.37μm.Astimewentby,thedamageg