简介：Determiningtheinfluencesofenvironmentalfactorsonseedlinggrowthandleafcolormayimproveourunderstandingofthechromogenicmechanismsinleavesandthebiosynthesisofanthocyanin.TodeterminetheoptimumconditionsforBetula'RoyalFrost'seedlingcultivation,weassessedseedlinggrowthandanthocyanincontentundernaturalconditions.Thetemperaturehadasignificantinfluenceonheightbutnotonradialgrowth.BetweenJuneandSeptember,theanthocyanincontentwassignificantlypositivelycorrelatedwithlightintensityandairmoistureandsignificantlynegativelycorrelatedwithtemperature.Insingle-factorexperimentstodeterminethevariationinanthocyanincontentinthegreenhouse,anthocyanincontentincreasedwithincreasingsoilwatercontentandatfirstincreased,thendecreasedwithincreasingpHoftheirrigationwaterandtemperature,butdecreasedwithincreasinglightintensity.K2SO4canincreasetheanthocyanincontent.Sotomaintainleafcolor,seedlingsshouldbecultivatedwith\200lmolm-2s-1lightintensityat20_Candsoilmoisturecontentbetween60and90%,andtheirrigationwatershouldbeneutraloralkaline.Additionally,sprayingwithK2SO4canbebeneficial.