简介:AbstractObjective:To investigate the relationship between the concentration of L-carnitine in semen and sperm parameters and investigate the epigenetic profile in sperm cell after L-carnitine usage.Methods:From February 2017 to February 2018, 46 semen samples from asthenospermic males and 41 semen samples from healthy donors were acquired. Motility parameters were assessed using computer-assisted sperm analysis (CASA, n = 78) and the DNA fragmentation index (DFI) was evaluated through flow cytometry (n = 86), %DFI = % cells outside main population. Other oxidative stress markers, such as reactive oxygen species (ROS) levels (n = 86) and the mitochondria DNA copy numbers, were detected (n = 78). The concentration of L-carnitine and acetyl-L-carnitine was detected (n = 82), and methylation was analyzed (n = 30). After that, we collected 13 fresh semen samples from asthenospermic males and 23 fresh semen samples from healthy donors. These samples were used in a freeze-thaw model that was used to determine whether adding L-carnitine could change sperm progressive motility (n = 23), apoptosis index (n = 9), and methylation analysis (n = 7). In total, we have done 13 asthenospermia samples for Western blot, and except for the poor Western result, we analyzed 6 samples for H3K9ac detection, 7 samples for H3K9m3 and H3K27m3 detection, and immunofluorescence (n = 3). Finally, we had recruited 30 volunteers, and they were given oral administration of L-carnitine for 3 months and then collected semen samples at different time points for methylation analysis.Results:The concentration of acetyl-L-carnitine is negatively correlated with the %DFI value (r2 = 0.1090; P = 0.0026), and the concentration of acetyl-L-carnitine is positively correlated with sperm forward motility (r2 = 0.0543; P = 0.0458) and ROS (r2 = 0.1854; P < 0.0001), and the acetyl-L-carnitine level is negatively correlated with %DFI in asthenospermia (r2 = 0.1701; P = 0.0066), and the level of acetyl-L-carnitine in asthenospermic semen is significantly lower than the normal group (P = 0.0419). In addition, this study indicates that adding L-carnitine significantly improved sperm motility (P = 0.0325) and reduced sperm apoptosis (P = 0.0032). Importantly, Western blotting (P = 0.0429) and immunofluorescence staining results showed that the addition of L-carnitine reduced H3K9Me3 methylation level in sperm, respectively. Furthermore, semen samples from asthenospermic patients had reduced methylation levels in a specific region (16thP= 0.0003; 17thP= 0.0016) of the brain-derived neurotrophic factor (BDNF) promoter. The 16th methylation decreased with age (r2 = 0.1564; P = 0.0306), and the 17th methylation was decreased after treatment with L-carnitine for 28 days (P = 0.0341).Conclusion:L-carnitine can reduce the %DFI and also affect the methylation of the histone modification marker in sperm as a possible epigenetic regulator.
简介:【摘要】 梅钢 1780 热轧 L2 过程控制计算机系统是由上海宝信软件股份有限公司自主集成开发的, L2 过程控制计算机系统中的加热炉燃烧控制模型在计算板坯温度时需要使用到加热炉 DCS 系统中的一些数据,同时需要将模型计算的相关结果下发到 L1 加热炉 DCS 系统,这就需在 L2 过程控制计算机系统与 L1 加热炉 DCS 系统之间进行数据通讯。 本文 主要 介绍了 在上海宝信软件的 iPlature 平台下,利用其 iMultiLink 技术实现 L2 过程控制 计算机系统与 L1 加热炉 DCS 系统通讯的方法。
简介:鄂尔多斯市第三中学 摘要: 从教接近三十年,班主任作了二十几年,深感班主任工作的重要和艰难。班主任工作是一个学校的重心,事无巨细;是一个班级的灵魂和表率,轨物范世,言传身教;是社会千万家长格外关注的对象,希望之所在。做得好,皆大欢喜,稍有失误,前功尽弃。随着社会的发展,班主任工作也迎来了各种挑战,既有思想方面的,也有基本素养方面的,还有社会方面的,所以班主任工作一路走来,虽然兢兢业业,勤勤恳恳,但也战战兢兢,如履薄冰,唯恐对不起学生的信任、对不起社会。多年来,怀着对班主任工作的一份敬畏、责任和担当,一如既往,工作一直做得不错,得到了学生、家庭、学校、社会的认可,几乎年年被批评为优秀班主任。 关键词:高中 优秀 班主任
简介:摘要:施工中吊 运 各种 零星材料、小型 周转材料 等 所采用的 料斗、吊笼 , 市场购置 的 或通用性能不强 、 或未针对起重机械的起重能力有的放矢地设计 , 从而造成资源浪费。本着安全、经济、适用的原则,本文作者据以往成熟的施工经验,在无 计算 案例的情况下查阅资料,结合塔吊的允许吊重、 吊 运材料的特点,论述如何设计计算 、 自制系列小型料斗 、吊笼 ,拟将其过程形成计算格式模块,供大家在制作不同尺寸料斗 器 具时参考,从而达到 : 事前 快捷 制定专项方案 ; 事中确保材料的吊装 运 的过程安全,事后各项检查有依据、有痕迹的目的。