简介：【摘要】随着我国养殖业向着规模化的方向转变，对于疫病防控的问题要加强重视，而且防控难度和潜在的风险越来越大。对于动物疫情的诊断来说，目前采用的先进监测技术越来越多，但由于流感病毒的增多，也对动物疫病的诊断带来了较多难题。针对这一问题，本文在开展动物疫病诊断的过程中，对如何有效运用Real-time PCR进行了深入探究。对于Real-time PCR来会所，优势在于可以大批量检测、操作便捷、特异性强以及灵敏性高，有非常显著的应用价值。

简介：【摘要】作为一种高致癌性反转录病毒，J亚群禽白血病病毒(Subgroup J of avian leukosisvirus，ALV-J)，可以导致各种肿瘤性疾病的发生，还会引起其他病毒感染。对于养禽业来说，禽白血病一种没有有效的防治措施，主要是通过净化禽白血病病毒，对禽白血病的流行和慢性进行抑制，但实际起到的效果不佳。在针对J亚群禽白血病病毒来说，需要采取有效的检测方法，其中，Real-time PCR检测方法有很多优势，如：可重复性好、灵敏度高、特异性强等，针对ALV-J进行检测有良好效果。

简介：我们允许的现在的算法与水彩颜料画外观的3D景色的即时显示。我们的途径提供视觉复杂性的适当简化，模仿水彩颜料的典型自然效果，；提供二种必要的画技术：wet-on-wet；wet-on-dry画。我们基于图象空间处理而非在准确模拟上专注于有效算法。这允许3D景色的即时显示。在一张动画期间，高frame-to-frame连贯能由于一个稳定的分割计划被完成。最后，我们无缝地从3D景色用信息把光滑的照明集成到水彩颜料图象。

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简介：Thereal-timedispatchingmodelonbussystemisstudiedinthisarticle.Realtimedispatchingcanresumetheplannedschedulequickly,andthentoensurethereliabilityofthepublictransportserviceandtowellraisetheservicequality.

简介：Renderingoflarge-scaleforestscenesisachallengingtask,whosehighlygeometriccomplexitywillputheavyburdenoncurrentgraphicshardware.Whennavigatingthescene,theoverallvisualresultisgenerallyconsideredasthecoreconcern.Anewmethodisproposedinthispaperforlarge-scaleforestrenderingusingclusteringandmergingstrategies.Ourmethodimprovestherenderingeffectbyclusteringpolygonsaccordingtothepointinformationwithrelationtoneighbours.Afastforestrenderingsystemisdevelopedaccordingly.Therelativetechniquesinthesystemcanimprovethevisualqualityondemandofdifferentapplications.

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简介：Three-dimensionelectronicchartdisplayinformationsystem(ECDIS)isoneofthenewdevelopingdirectionsofelectronicnavigationchart,anditsreal-timequalityisoneoftheimportantrequirements.Inthispaperthealgorithmofsimplifyingtrianglesurfaceusingedgecontraction,whichcanensurethereal-timedisplayofobjectsinseabedenvironment,wasputforward.TheoptimumcontractionpointwasdeterminedbytheLagrangematrix,sothatthealgorithmensuresthatthecontractionpointlocatesontheoriginalmodel,meanwhilemeetsthedemandsofleastdeviation.Thisalgorithmcanimprovetheplottingspeed,andpreservetheboundarycharacterbyusingthefewertrianglestosimulateobjects.

简介：AbstractBackground:To detect acute schistosomiasis, low-intensity infections, or to verify the success of treatment with praziquantel, highly sensitive test methods are required. The aim of this study was therefore to demonstrate the performance of Schistosoma mansoni specific DNA detection in serum and urine using real-time polymerase chain reaction (PCR) in an endemic area before and after treatment.Methods:The study pursued a 1-week and 20-weeks longitudinal design with a treatment intervention among 36 study participants aged 18 to 70 years in the community of Kayenze, a fishing village in Ilemela district on the southern shore of Lake Victoria in north-western Tanzania between February and June 2018. Blood, urine and stool samples were collected from each participant to diagnose Schistosoma mansoni infection before and two times after treatment with praziquantel using serum-and urine based real-time PCR, point-of-care circulating cathodic antigen (POC-CCA) rapid diagnostic test and the microscopic Kato-Katz (KK) method. Kappa coefficient (κ) was used to estimate the agreement between these diagnostic tests compared to a combined "gold standard" of positive results by serum-based real-time PCR and/or positive egg counts determined by KK. Kendall’s Tau rank correlation was used to examine the relationship between cycle threshold (Ct)-values and egg counts and the Wilcoxon signed-rank test was used to compare the median Ct-values of the different examination time points.Results:By using the combined "gold standard" of the parasitological Kato-Katz test and/or serum-based real-time PCR, a S. mansoni prevalence of 77.1% could be determined at baseline. In terms of sensitivity, serum-based realtime PCR (96.3%) and POC-CCA assay (77.8%) showed the highest results. The detection of DNA from urine samples showed the lowest sensitivity (33.3%). Treatment with praziquantel resulted in a significantly reduced prevalence of S. mansoni. No infection could be detected by Kato-Katz, with the POC-CCA test only 33.3%. The analysis of the median Ct values over time (which were determined by the serum-based real-time PCR) showed that the Ct decreases significantly shortly after treatment (from 30.3 to 28) and increases above baseline level (34.9) three months later.Conclusions:The data presented here show that the serum-based real-time PCR exhibits excellent diagnostic accuracy, in contrast to the use of urine as sample material for S. mansoni DNA detection. However, as circulating DNA does not necessarily reflect the persistence of living worms in schistosomiasis, this method is less well suited to verify the success of treatment with praziquantel.

简介：Actionrefinementforreal-timeconcurrentprocesseswithurgentinteractionsisstudied,whereapartial-ordersetting,i.e.,timedbundleeventstructures,isusedasthesystemmodelandareal-timeLOTOS-likeprocessalgebraisusedasthespecificationlanguage.Itisshownthattheproposedrefinementapproacheshavethecommonlyexpectedproperties:(1)thebehaviouroftherefinedprocesscanbeinferredcompositionallyfromthebehaviouroftheoriginalprocessandfromthebehaviouroftheprocessessubstitutedforactions;(2)thetimedextensionsofpomset(partiallyorderedmultiset)traceequivalenceandhistorypreservingbisimulationequivalencearebothcongruencesundertherefinement;(3)thesyntacticandsemanticrefinementscoincideuptotheaforementionedequivalencerelationswithrespecttoacpo-baseddenotationalsemantics.

简介：Thenoisedatainverticalcomponentrecordsof85seismicstationsinFujianProvinceduring2012isusedastheresearchobjectinthispaper.Thenoisedataisdividedintofiveminutesegmentstocalculatethepowerspectra.Thehighreferencelineandlowreferencelineofstationarethenidentifiedbydrawingaprobabilitydensityfunctiongraph(PDF)usingthepowerspectralprobabilitydensityfunction.Moreover,accordingtotheanomaliesofPDFgraphsin85seismicstations,theabnormalnoiseisdividedintofourcategories:droppedpacket,lownoise,highnoise,andmediannoiseanomalies.Afterwards,fourselectionmethodsarefoundbythehighorlownoisereferencelineofthestations,andthesystemofreal-timemonitoringofseismicnoiseisformedbycombiningthefourselectionmethods.Noiserecordsof85seismicstationsinFujianProvinceinJuly2013areselectedforverification,andtheresultsshowthattheanomalousnoise-recognitionsystemcouldreacha90%successrateatmoststationsandtheeffectofselectionareverygood.Therefore,itcouldbeappliedtotheseismicnoisereal-timemonitoringinstations.

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简介：Thispaperdescribesamulti-threatreal-timeseparatingsystemforbroadbandanti-radiationmissileseeker.Itpresentsamethod,withadual-portmemoryascomparer,toperformPFandPWhardwarereal-timeseparationandtodeterminethetime-of-arrival(TOA)byuseofsequentialdifferencehistogram(SDIF).Themethodhasbeenappliedtopractice,whichhasachievedgoodresults.

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简介：Complexurbansceneryisgenerallycomposedofgiganticamountofdetailedbuildings,efficientrepresentationandrenderingareessentialforitsvisualization.Wepresentanacceleratingmethodforurbanvisualization.Ourapproachcanoptimizetheorganizationofmodelsinaccordancewiththequadtreebasedterrain,whichmakestheparallelizationeasier.Throughminimizingthedrawcallwithinonerenderingprocess,ourapproachcanreducethetimecostofeachframeandimprovetheframerategreatly.Hereby,oursystemcanhandlelarge-scaledetailedmodelswithhighdiversityandalsocanaffordtheabilitytoadjusttherenderingstrategyautomaticallyaccordingtothestateofthehardware.

简介：ThetraditionalorientedFASTandrotatedBRIEF(ORB)algorithmhasproblemsofinstabilityandrepetitionofkeypointsanditdoesnotpossessscaleinvariance.Inordertodealwiththesedrawbacks,amodifiedORB(MORB)algorithmisproposed.Inordertoimprovetheprecisionofmatchingandtracking,thispaperputsforwardanMOKalgorithmthatfusesMORBandKanade-Lucas-Tomasi(KLT).ByusingKalman,theobject’sstateinthenextframeispredictedinordertoreducethesizeofsearchwindowandimprovethereal-timeperformanceofobjecttracking.TheexperimentalresultsshowthattheMOKalgorithmcanaccuratelytrackobjectswithdeformationorwithbackgroundclutters,exhibitinghigherrobustnessandaccuracyondiversedatasets.Also,theMOKalgorithmhasagoodreal-timeperformancewiththeaverageframeratereaching90.8fps.

简介：Thispaperanalyzesthecurrentdifficultiesencounteredinon-lineinspectionsystemsofstripsurfacequality,specificallyrelatingtoproblemswithreal-timeprocessingofhugeamountsofdata.Toaddressthisneed,thispaperdescribesanFPGA-basedhigh-speedimageprocessingmodulewithbothhardwareandsoftwareaspects.Improvingthesetwoaspectstogetherwillhelpthesystemachievereal-timeprocessingofmassiveimagedata,andsimplifiesthearchitectureofthestripsurfacequalityon-lineinspectionsystem.

简介：Simulationandrenderingoflarge-scalenaturalenvironments,especiallytheocean,hasalwaysbeenoneofthehotissuesincomputergraphics,whichcanproviderealismforvariousapplicationssuchascomputergame,movieandmilitaryusage.Simulationofoceanenvironmentisoftenlackofrealismforreal-timeapplicationduetoitscomplexityofdynamicwaves.Inthispaper,amethodbasedonFFTWavemodelisproposedtosolvethisproblem,whichcanalsosimulatetheoceanopticpropertywithatmospherescattering.Furthermore,ourmethodhasalotofadvantagesincludingglobaloceandatasetsupport,real-time,dynamicreflectionofocean,thefoamonthewave,smoothtransitionfromdeepoceantoseacoast,etc.Theexperimentalresultsdemonstratetherealismandeffectivenessofourapproach.

简介：ThispaperextendstheSPHmethodtogassimulation.TheSPH(SmoothedParticlesHydrodynamics)methodisthemostpopularmethodofflowsimulation,whichiswidelyusedinlarge-scaleliquidsimulation.However,itisnotfoundtoapplytogassimulation,sincethosemethodsbasedonSPHcan’tbeusedinreal-timesimulationduetotheirenormousparticlesandhugecomputation.ThispaperproposesamethodforgassimulationbasedonSPHwithasmallnumberofparticles.Firstly,themethodcomputesthepositionanddensityofeachparticleineachpoint-in-time,andoutlinestheshapeofthesimulatedgasbasedonthoseparticles.Secondlythemethodusesthegridtechniquetorefinetheshapewiththediffusionofparticle’sdensityunderthecontrolofgrid,andgetmorelifelikesimulationresult.Eachgridwillbeassigneddensityaccordingtotheparticlesinit.Thedensitydeterminesthefinalappearanceofthegrid.Forensuringthenaturaltransitionofthecolorbetweenadjacentgrids,wegiveadiffuseprocessofdensitybetweenthesegridsandassignappropriatevaluestovertexesofthesegrids.Theexperimentalresultsshowthattheproposedmethodcangivebettergassimulationandmeettherequestofreal-time.

简介：Theconventionaldatawarehousecanonlyanalyzehistoricaldata.Thispaperproposesaconceptofagent-basedreal-timedatawarehouseinBIsystem,Withthismodelwecanimprovethereal-timeperformanceofdatawarehouse.Ontheotherhand,theBIsystemflexibilityisenhancedinthismodel,whichmakesitmuchmoreefficienttomanagethedistributedenvironment.