简介:飞机损伤检测数据库系统是根据飞机损伤检测需要设计实现的一个数据库web应用系统,通过本系统可以便捷的进行飞机损伤检测数据的存储、管理、检索等工作,为数据分析奠定基础。系统采用B/S结构,位于服务器的Web应实现web应用程序对数据库的操作;为了避免检索结果数据量较大时占用过多网络资源并影响系统的响应速度,采用存储过程分页技术实现数据分页用程序采用ASP.NET技术开发,并将其划分为表示层、业务逻辑层和数据访问层;使用SQLServer进行试验数据管理,并通过ADO.NET技术功能;为了有效利用已有Word格式的数据资源,并生成标准化Word文档报告,系统提供了Word格式文件的导入导出接口。
简介:Themajormatesterilegenesinanewphoto/thermo-sensitivegenicmalesterile(PTGMS)lineB06Sofricewereanalyzedbythemanipulationofmixturedistributiontheory.TheresultsindicatedthatapairofmajormalesterilenucleargeneswithlargeeffectswereresponsibleforcontrollingthemalesterilityofB06S.
简介:ThepurposeofthisstudywastoconstructaneukaryoticDNAvectorencodingamultipleepitopeantigen(MFC)ofhepatitisCvirus(HCV)andahepatitisBsurfaceantigen(HBsAg),andexploretheeffectofHBsAggeneontheimmunityofHCVmultiple-epitopeDNAconstructinvitroandinvivoinmice.AnHCVDNAvector(pVAX1-HBs-MFC)wasconstructedbyfusingHBsAggenetotheNterminalofanHCVmultiple-epitopeantigengene.ThepVAX1-HBs-MFCwastransfectedintoHEK293TcellsanditsexpressionwasmeasuredbyELISAandWesternblotting.BALB/cmicewereintramuscularlyimmunizedwiththepVAX1-HBs-MFC,andanELISAapproachwasappliedtodeterminethespecificantibodytitersandsubtypesinthemouseserum.Thecross-reactivityoftheantibodieswasalsocheckedwithtwosynthesizedHCVhypervariableregion1(HVR1)peptides.TheIFN-γproductionandcellproliferationofthemousespleencellswereevaluatedbyELISAandMTS(3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2H-tetrazolium,innersalt)assays,respectively.TheexpressionofpVAX1-HBs-MFCwasdetectableinthetransfectedHEK293Tcells.TheserumantibodyresponsewaseffectivelyelicitedinBALB/cmiceinjectedwithpVAX1-HBs-MFC.ThehighesttiterofantibodyagainstHCV(MFC)was1:1280,andtheratioofIgG2a/IgG1was1.50±0.12atthefifthweekafterfirstimmunization.Moreover,thecollectedmouseserumantibodyhadtheabilitytocross-reactwiththetwosynthesizedHCVHVR1peptides.ThestimulationindexofthemousesplenocytestoMFCwas1.79±0.07,andtheIFN-γlevelwas287±6pg/mlatweek21afterfirstimmunization.ThehighesttiteroftheantibodyincontrolBALB/cmiceimmunizedwithpVAX1-MFCwas1:320,andtheratioofIgG2a/IgG1was1.33±0.11atweek5post-immunization.Furthermore,thestimulationindexofthemousesplenocytescellstoMFCwas1.52+0.06,andtheIFN-γlevelwas225±9.3pg/mlatweek21post-immunization.TheHBsAggenecanenhancetheeffectsofanHCVmultiple-epitope
简介:""Mensaytheyhatetoshop,""saysZhukin,CityUniversityofNewYorkSociology(社会学)professor.""Yetwhenyouaskthemdeeperquestions,itturnsoutthattheyliketoshop.Mengenerallyliketoshopforbooks,musicandhardware.Butifyouaskthemabouttheshoppingtheydoforbooksormusic,they'llsay,'Well,that'snotshopping.That'sresearch.'""