简介:摘要目的建立三七丹参片中人参皂苷Rg1、Rb1和三七皂苷R1的含量测定方法。方法高效液相色谱法;色谱柱为AgiLentHypersiLC18柱(4.0×250mm,5μm),以乙腈为流动相A,以水为流动相B,梯度洗脱,流速1ml/min,柱温25℃,检测波长为203nm。结果人参皂苷Rg1进样量为0.1152~0.96mg/ml,与峰面积呈良好线性关系(R2=0.9995),平均回收率为100.68%,平均峰面积(RSD)为2.77%,人参皂苷Rb1进样量为99.96~833μg/ml,与峰面积呈良好线性关系(R2=0.9996),平均回收率为97.17%,平均峰面积(RSD)为1.98%,三七皂苷R1进样量为22.32~186μg/ml,与峰面积呈良好线性关系(R2=0.9997),平均回收率为99.82%,平均峰面积(RSD)为1.87%。结论所建立的方法简便、准确、重复性好,可用于三七丹参片中的人参皂苷Rg1、Rb1和三七皂苷R1含量测定。
简介:目的研究上海地区血压正常汉族老年人中(年龄≥60岁)Ⅰ型血管紧张素Ⅱ受体(AT1-R)基因A1166C多态性的分布特点,并同国内外人群的分布频率相比较,了解地区种族分布差异及与年龄、体重指数BMI(kg/m2)的相互关系.方法体检选取92例正常健康老年人,男72例,女20例,平均年龄65.32±4.70岁.应用PCR-RFLP方法检测位于AT1-R基因3'-UTR的A1166C变异.结果(1)上海地区血压正常汉族老年人AA、AC、CC基因型频率分别为0.89、0.11、0.00,1166C等位基因频率为0.05.低于白种人和日本人(P《0.001和P=0.046),与美国黑人相比无差异(P=0.815).(2)在国内人群中C1166等位基因的分布频率无差异,并与年龄、体重指数无关.结论AT1-R基因A1166C多态性存在种族地域间的差异,上海地区与国内现有报道结果一致,1166C等位基因频率明显低于白种人群,但不同年龄不同体重指数人群1166C等位基因频率无差别.提示由于中国人群1166C等位基因频率低,故开展AT1-R基因A1166C多态性与疾病的关系中需要大样本研究.
简介:本文介绍了微软的一些新消息。包括WindowsInTune、MCS14、Server2008SPl以及Windows7和IE9HTML5兼容性等。
简介:THENEWCARCHALLENGE新上市的宝马5系固然很棒,但你们不觉得这类车型越来越中庸,越来越无聊了吗?对于骨子里是个不安分的人来说,花差不多的钱绝对会选择辣味十足的钢炮,比如今天对决的这两款.自我介绍高尔夫家族最最强悍的车型思域家族最最强悍的车型亮点已经是最快的“神车”了,还需要别的亮点吗在人人都以刷纽博格林赛道纪录为荣的当下,我们吃不准新思域TypeR是不是最快量产前驱者纪录的创造者或者保持者,只知道它的成绩是7分43.8秒.
简介:目的:建立舒胸片中人参皂苷Rg1、人参皂苷Rb,和三七皂苷R1含量的HPLCI]定方法。方法:采用DiamosilC18柱(4.6X250mm,5gm),流动相乙腈一水,梯度洗脱,柱温30℃,流速lmL/min,203nm波长下检测。结果:人参皂苷Rg1、人参皂苷Rb1、三七皂苷R,分别在0.442~11.050gg(r=0.9999)、0.344~8.600μg(P=0.9999)、0.208~5.200gg(r=0.9995)范围内线性关系良好,平均回收率分别为98.93%(RSD为1.7%1、98.88%(RSD为1.4%)、98.98%(RSD为1.4%)。结论:以HPLC法检测舒胸片中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1,方法简便、准确、灵敏度高、重复性好。
简介:AbstractBackground:Breast cancer (BC) is a common malignancy with highly female incidence. So far the function of notoginsenoside R1 (NGR1), the extract from Panax notoginseng, has not been clearly elucidated in BC.Methods:Optimal culture concentration and time of NGR1 were investigated by cell counting kit-8 assay. Cell proliferation ability was measured by colony formation assays. Transwell assay was used to detect the effect of NGR1 on cell migration and invasion. The apoptosis rate of cells between each group was measured by TUNEL assay.Results:NGR1 treatment has an inhibitory effect on proliferation, migration, invasion, and angiogenesis and a stimulating effect on cell cycle arrest and apoptosis of Michigan Cancer Foundation-7 (MCF-7) cells. The 50% growth inhibitory concentration for MCF-7 cells at 24 h was 148.9 mmol/L. The proportions of MCF-7 cells arrested in the G0/G1 phase were 36.94±6.78%, 45.06 ± 5.60%, and 59.46±5.60% in the control group, 75, and 150 mmol/L groups, respectively. Furthermore, we revealed that NGR1 treatment attenuates BC progression by targeted downregulating CCND2 and YBX3 genes. Additionally, YBX3 activates phosphatidylinositol 3-phosphate kinase (PI3K)/protein kinase B (Akt) signaling pathway by activating kirsten rat sarcoma viral oncogene, which is an activator of the PI3K/Akt signaling pathway.Conclusion:These results suggest that NGR1 can act as an efficacious drug candidate that targets the YBX3/PI3K/Akt axis in patients with BC.
简介:摘要目的探究三七皂苷R1对老龄大鼠学习记忆的作用及其机制。方法20月龄SD雄性大鼠30只,以每组10只随机分为溶剂对照组、三七皂苷R1低剂量(5mg?kg-1)组和高剂量(10mg?kg-1)组,另取2月龄SD雄性大鼠10只为正常对照组。采用Morris水迷宫实验记录老龄鼠的行为变化。Westernbolt法测量PKA、P-CREB、BDNF含量。结果三七皂苷R1能缩短老龄大鼠的逃避潜伏期并增加其穿越平台次数,使老龄大鼠脑组织中的BDNF的蛋白表达水平增高。结论三七皂苷R1能改善老龄大鼠的学习记忆能力。BDNF信号通路是其作用机制之一。
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简介:Amajorproblemwhichispoorlyunderstoodinthemanagementofbladdercancerislowsensitivitytochemotherapyandhighrecurrenceaftertransurethralresection.Insulin-likegrowthfactor1receptor(IGF-1R)signalingplaysaveryimportantroleinprogression,invasionandmetastasisofbladdercancercells.Inthisstudy,weinvestigatedwhetherIGF-1Rwasinvolvedinthegrowthstimulatingactivityanddrugresistanceofbladdercancercells.Theresultsshowed:ThemRNAsofIGF-1,IGF-2andIGF-1Rwerestronglyexpressedinserum-freeculturedT24cellline,whereasnormalurothelialcellsdidnotexpressthesefactors/receptorsoronlyintracelevels;T24cellrespondedfarbettertogrowthstimulationbyIGF-1thandidnormalurothelialcells;blockageofIGF1Rbyantisenseoligodeoxynucleotide(ODN)significantlyinhibitedthegrowthofT24cellandenhancedsensitivityandapoptosisofT24cellstomitomycin(MMC).TheseresultssuggestedthatblockageofIGF-IRsignalingmightpotentiallycontributetothetreatmentofbladdercancercellswhichareinsensitivetochemotherapy.