简介:摘要目的探讨miR-301a-3p对大鼠星形胶质细胞凋亡与增殖的影响。方法获取并培养大鼠原代星形胶质细胞,将miR-301a-3p agomir以及antagomir分别转染至细胞。分Blank组、miR-NC对照组、miR-301a组、antagomir组,每组设3个复孔,每孔2×105个细胞,CCK8实验检测各组细胞的增殖能力变化;用流式细胞术及Caspase-3活性检测分析细胞凋亡的情况。结果与Blank组(48 h:0.83±0.09;72 h:1.20±0.21;96 h:1.65±0.17)和miR-NC组(48 h:0.79±0.10;72 h:1.12±0.25;96 h:1.60±0.15)相比,miR-301a组(48 h:1.16±0.07;72 h :1.56±0.11;96 h:2.13±0.14)细胞的增殖能力显著提高(P<0.05),miR-301a组细胞的凋亡率显著下降(Blank组:10.44±1.33,miR-NC组:9.84±1.40,miR-301a组:4.32±0.51,P<0.05);而antagomir组(48 h:0.52±0.12;72 h:0.72±0.09;96 h:1.01±0.15)细胞转染后增殖能力较Blank组和miR-NC组显著降低(P<0.05),antagomir组细胞的凋亡率显著升高(Blank组:10.44±1.33,miR-NC组:9.84±1.40,antagomir组:21.41±2.57,P<0.05)。结论miRNA-301a-3p过表达促进大鼠星形胶质细胞的增殖,抑制细胞的凋亡通路,降低细胞的凋亡,从而调控大鼠星形胶质细胞的生物学功能。
简介:由两位出色的时装设计师为Cavalli打造了梦幻多彩IPECavalli品牌。黑色、红色、白色、金属、玻璃、皮革、毛料。。。大胆、妖艳、冷酷、爱不释手都是她所带给你的感觉。
简介:散场的人群逐渐离去后,杨景泉慢悠悠地从洗手间走出来。从二楼音乐厅走下来的时候,听见大厅里传来两个男人聊天的声音,在回音嗡嗡作响的圆弧形场馆中,完全听不清他们在讲什么。白色大灯已经关闭,只留下走道两侧的烛台形壁灯和从天花板倾泻而下的巨型水晶吊灯。水晶吊灯落下的影子,完满地对应大厅地面正中的圆形团花图案,吻合对称得叫人惊叹。杨景泉的皮鞋叩击楼梯的声音使得两个聊天的男人同时看向他。杨景泉觉得双腿有些不自然,身上发热。已经四月底了,他依然坚持着看古典音乐会一定要穿西装,即使夏天也是如此。热不是西装的原因,杨景泉想,是我太胖了,一米七八的身高,体重已经达到一百一十公斤。实在不喜欢运动,回家后半卧在沙发上听音乐,这时的时间过得最快,也是最合心意的。
简介:<正>Lecielrapprochesonvisage,level’immunitedenotreoubli.Defortespresomptionsnousalterent,nousrejettentanonymedanslacirculationplusepaisse.Onestheureuxdanslescombatsquel’onprepal’hiverfourbitunaeierderegards;laconvoitisedelaneigenenoussurprendrapasfrileux.
简介:Newmetal-freeorganicdyesensitizerscontainingmono-triphenylamineorbis-triphenylamineastheelectrondonor,athiopheneastheπ-conjugatedsystem,andacyanoacrylicacidmoietyastheelectronacceptorweresynthesized.Theopticalandelectrochemicalpropertiesofthedyeswereinvestigated,andtheirperformanceassensitizersinsolarcellswasevaluated.Dye-sensitizedsolarcellsbasedondyecontainingbis-triphenylamineastheelectrondonorproducedaphoton-to-currentconversionefficiencyof6.06%(Jsc=14.21mA/cm~2,Voc=0.62V,ff=0.69)under100mW/cm~2simulatedAM1.5Gsolarirradiation(100mW/cm~2).
简介:X-rayimagingistheconventionalmethodfordiagnosingtheorthopedicconditionofapatient.ComputerizedTomography(CT)scanningisanotherdiagnosticmethodthatprovidespatient’s3Danatomicalinformation.However,bothmethodshavelimitationswhendiagnosingthewholeleg;X-rayimagingdoesnotprovide3Dinformation,andnormalCTscanningcannotbeperformedwithastandingposture.Obtaining3Ddataregardingthewholeleginastandingpostureisclinicallyimportantbecauseitenables3Danalysisintheweightbearingcondition.Basedontheseclinicalneeds,ahardware-basedbi-planeX-rayimagingsystemhasbeendeveloped;itusestwoorthogonalX-rayimages.However,suchmethodshavenotbeenmadeavailableingeneralclinicsbecauseofthehightcost.Therefore,weproposedawidelyadaptivemethodfor2DX-rayimageand3DCTscandata.Bythismethod,itispossibletothreedimensionallyanalyzethewholeleginstandingposture.TheoptimalpositionthatgeneratesthemostsimilarimageisthecapturedX-rayimage.Thealgorithmverifiesthesimilarityusingtheperformanceoftheproposedmethodbysimulation-basedexperiments.Then,weanalyzedtheinternal-externalrotationangleofthefemurusingrealpatientdata.Approximately10.55degreesofinternalrotationswerefoundrelativetothedefinedanterior-posteriordirection.Inthispaper,wepresentausefulregistrationmethodusingtheconventionalX-rayimageand3DCTscandatatoanalyzethewholelegintheweight-bearingcondition.
简介:目的:研究DM301大孔树脂对大黄总葸醌的吸附性能及分离纯化工艺参数。方法:以大黄总蒽醌含量为评价指标,采用紫外分光光度法测定大黄总蒽醌的含量。结果:DM301大孔树脂对大黄总蒽醌适宜的交换吸附条件为:以10g大孔吸附树脂为标准量,最大上样量为3.4g·g^-1(生药材/干树脂),最佳上柱工艺为药液浓度含生药量0.40g·mL^-1,pH6,流速为1mL·min^-1,90%乙醇洗脱,洗脱液用量为80mL。验证实验证明,DM301对大黄总蒽醌的动态吸附率在70%以上,洗脱率在80%以上。结论:DM301大孔树脂交换吸附大黄总蒽醌的纯化方法可取,具有良好的应用前景。