简介:AIM:Topresentretinalmicrostructure,metabolismandfunctionabnormalitiesinthecourseofmultipleevanescentwhitedotsyndrome(MEWDS)byHeidelbergspectralismodalityimagingplatformandobserveitsoutcomebyEDI-SD-OCTandtwowavelengthautofluorescence.METHODS:Acaseofmultipleevanescentwhitedotsyndromeina23-year-oldfemalepresentedinitiallywitha15-dayhistoryoffloatersandacentralscotomaintherighteye.Toestablishthediagnosis,multimodalityimagingwasperformed,namely,bluelight-fundusautofluorescence(BL-FAF,excitation488nm,emission>500nm),near-infraredfundusautofluorescence(NIR-FAF,excitation787nm,emission>800nm)usingaconfocalscanninglaserophthalmoscope,fundusfluoresceinangiography(FFA),indocyaninegreenangiography(ICGA),spectrum-domainenhancedepthimagingopticalcoherencetomography(SD-EDI-OCT),multifocalelectroretinography(mf-ERG)andfundusphotograghwereperformedandfollowedupattheeighthmonthafterinitiallyvisiting.RESULTS:Opticalcoherencetomography(OCT)showedatransientdisruptionofthefovealphotoreceptoroutersegmentsincorrespondencetofovealgranularity.NIR-FAFshowedhypoautofluorescentareas,≤40μminsize,mostlyconcentratedaroundtheposteriorpoleanditstemporalsidelessthanthatinBL-FAF.Mf-ERGshowpinnacledisappearedinfoveaandmaculaandresponsesdecreasedmarkedlycomparedwiththefolloweye.Attheeighthmonthfollowup,hyperfluorescenceinBL-FAFweredisappear,while,NIR-FAFHypofluorescentspotsinearlystageofsuchlesionwerereduced.ButOCTdemonstratedthestructurewasrecoveredinresidualHypofluorescentareainNIR-FAF.Thesubfovealchoroidalthicknesswasdecreasedfrom372μmto307μmslightlyandcostlinewasrecovered.CONCLUSION:MEWDSisabenignself-healingdiseaseandthereisnopathologicalevidencetoinvestigatethenaturalcourseofsuchdisease.SD-OCTallowshighlydetailedimagesapproachinghistopathologytocertifythemicrostructura
简介:2011年是列宁《论战斗唯物主义的意义》发表90周年。列宁强调,社会主义文化建设的主体力量,是党员与党外人士、哲学家与自然科学家、当今的马克思主义者同历史上唯物主义哲学家结成的三种联盟;社会主义文化建设的舆论引导,要重视反宗教宣传,加强无神论教育;社会主义文化建设的现实保障,是无产阶级学会运用政权,发挥社会主义的制度优势;社会主义文化建设的科学方法,要凸显事实教育,改进马克思主义教育方法。列宁发出了社会主义文化建设的历史先声,提出了推动社会主义文化发展繁荣的庄严号召。这对现时代推动社会主义文化大发展大繁荣具有长远的指导意义。
简介:目的:应用PCR-变性梯度凝胶电泳技术(denaturinggradientgelelectrophoresis,DGGE)技术检测早期儿童龋治疗前后唾液中微生物多样性的变化。方法:选取北京大学燕东幼儿园22名3-5岁儿童进行口腔检查,在龋齿治疗前和治疗后2周分别取样,采用PCR-DGGE技术检测受试儿童唾液样本,分析治疗前后唾液微生物多样性的变化。结果:治疗前唾液样本DGGE条带数为23±2.9,治疗后唾液样本DGGE条带数为28±4.1,差异有显著性(P〈0.01);在同一时期内各唾液样本DGGE图谱的相似性高于不同时期样本之间。结论:PCR—DGGE技术可用于与龋病相关细菌的生物多样性的研究以及监测微生物群落组成的动态变化。