Haplotyping of Rice Genotypes Using Simple Sequence RepeatMarkers Associated with Salt Tolerance

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摘要 Saltstressisamajorprobleminmostofthericegrowingareasintheworld.AmajorQTLSaltolassociatedwithsalttoleranceattheseedlingstagehasbeenmappedonchromosome1inrice.ThisstudyaimedtocharacterizethehaplotypediversityatSaltolandadditionalQTLsassociatedwithsalttolerance.Salttoleranceattheseedlingstagewasassessedin54ricegenotypesinthescaleof1to9scoreatEC=10dSm-1undercontrolledenvironmentalconditions.SevennewbreedinglinesincludingthreeKMR3/O.rufipogonintrogressionlinesshowedsimilarsalttolerantabilityasFL478andcanbegoodsourcesofnewgenes/allelesforsalttolerance.Simplesequencerepeat(SSR)markerRM289showedonlytwoallelesandRM8094showedsevenalleles.Polymorphicinformationcontentvaluevariedfrom0.55forRM289to0.99forRM8094andRM493.Basedon14SSRmarkers,the54lineswereclearlyseparatedintotwomajorclusters.FourteenhaplotypeswereidentifiedbasedonSaltollinkedmarkerswithFL478asthereference.AllelesofRM8094andRM3412candiscriminatebetweenthesalttolerantandsusceptiblegenotypesclearlyandhencecanbeusefulinmarker-assistedselectionattheseedlingstage.OthermarkersRM10720onchromosome1andRM149andRM264onchromosome8canalsodistinguishtolerantandsusceptiblelinesbutwithlesserstringency.
机构地区 不详
出处 《水稻科学:英文版》 2016年6期
关键词 水稻科学 农业
出版日期 2016年06月16日(中国期刊网平台首次上网日期,不代表论文的发表时间)
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