简介:目的:通过对Q值调整非球面切削与标准化LASIK术后视觉质量的观察来分析Q值引导个性化切削技术的临床效果。方法:对2008-01/05等效球镜低于-12.00D的46例88眼进行非球面切削或标准化LASIK术后进行随访,按术前屈光度数将其分为A组(〈-6.00D)和B组(-6.00~-12.00D)两组,对比各组非球面切削与标准化LASIK术后1mo裸眼视力、Q值、夜间眩光、夜间视物质量及满意率(后3者采取问卷调查)。结果:A、B组内非球面切削与标准化LASIK术后裸眼视力差异无统计学意义(t=1.18,P〉0.05;t=0.62,P〉0.05);A、B组内非球面切削与标准化LASIK术后Q值差异无统计学意义(t=-0.93,P〉0.05;t=-0.372,P〉0.05);A组内非球面切削与标准化LASIK术后眩光、夜间视物质量差异无统计学意义(眩光:χ^2=2.397,P>0.05;夜间视物质量:χ2=1.263,P〉0.05);B组内非球面切削与标准化LASIK术后眩光、夜间视物质量差异有统计学意义(眩光:χ^2=10.85,P〈0.01;夜间视物质量:χ^2=10.85,P〈0.01);术后效果满意率达到98%。结论:非球面切削能明显改善-6.00D以上的术后主观视觉质量。
简介:AIM:ToidentifythefunctionofST2andexploretheroleofIL-33/ST2signalinginregulatingthepro-allergiccytokineproductioninhumancornealepithelialcells(HCECs).METHODS:HumancornealtissuesandculturedprimaryHCECsweretreatedwithIL-33indifferentconcentrationswithoutorwithdifferentinhibitorstoevaluatetheexpression,locationandsignalingpathwaysofST2inregulatingproductionofpro-allergiccytokineandchemokine.TheexpressionofmRNAwasdeterminedbyreversetranscriptionandrealtimePCR,andproteinproductionwasmeasuredbyenzyme-linkedimmunosorbentassay(ELISA),immunohistochemicalandimmunofluorescentstaining.ST2proteinwasdetectedindonorcornealepithelium,andST2signalwasenhancedbyexposuretoIL-33.·RESULTS:IL-33significantlystimulatedproductionofpro-allergiccytokinesthymicstromallymphopoietin(TSLP)andchemokine(CCL2,CCL20,CCL22)inHCECsatbothmRNAandproteinlevels.Thesestimulatedproductionsofpro-allergicmediatorsbyIL-33wereblockedbyST2antibodyorsolubleST2protein(P<0.05).Interestingly,theIκB-αinhibitorBAY11-7082orNF-κBactivationinhibitorquinazolineblockedNF-κBp65proteinnucleartranslocation,andalsosuppressedtheproductionsofthesepro-allergiccytokinesandchemokineinducedbyIL-33.CONCLUSION:ThesefindingsdemonstratethatIL-33/ST2signalingplaysanimportantroleinregulatingIL-33inducedpro-allergicresponses.IL-33andST2couldbecomenovelmoleculartargetsfortheinterventionofallergicdiseasesinocularsurface.
简介:AIM:Todescribethecharacteristicsofmodulationtransferfunction(MTF)ofanteriorcornealsurface,andobtainthethenormalreferencerangeofMTFatdifferentspatialfrequenciesandopticalzonesoftheanteriorcornealsurfaceinmyopes.·METHODS:Fourhundredeyesfrom200patientswereexaminedunderSIRIUScornealtopographysystem.PhoenisanalysissoftwarewasappliedtosimulatetheMTFcurvesofanteriorcornealsurfaceatverticalandhorizontalmeridiansatthe3,4,5,6,7mmopticalzonesofcornea.TheMTFvaluesatspatialfrequenciesof5,10,15,20,25,30,35,40,45,50,55and60cycles/degree(c/d)wereselected.·RESULTS:TheMTFcurveofanteriorcornealsurfacedecreasedrapidlyfromlowtointermediatefrequency(0-15cpd)atvariousopticalzonesofcornea,thevaluedecreasedto0slowlyathigherfrequency(>15cpd).Withtheincreaseoftheopticalzonesofcornea,MTFcurvedecreasedgradually.3)Intherangeof3mm-6mmopticalzonesofthecornea,theMTFvaluesmeasuredathorizontalmeridianweregreaterthanthecorrespondingvaluesathorizontalmeridianofeachspatialfrequency,thedifferencewasstatisticallysignificant(P<0.05).At7mmopticalzonesofcornea,theMTFvaluesmeasuredathorizontalmeridianwerelessthanthecorrespondingvaluesatverticalmeridianat10-60spatialfrequencies(cpd),andthedifferencewasstatisticallysignificantin25,30,35,40,45,50cpd(P<0.05).·CONCLUSION:MTFcanbeusedtodescribetheimagingqualityofopticalsystemsatanteriorcornealsurfaceobjectivelyindetail.
简介:<正>DearSir,IamDrZheSong,fromDepartmentofOphthalmologyofDongfangHospitalAffiliatedtoBeijingUniversityofChineseMedicineinBeijing,China.Iwritetopresentacasereportofthetreatmentofcomplextraumaticretinadetachment,whichhasthreehugebreaks.A35-year-oldman,whowasknockeddownandhurthisrighteyewhileplayingfootball,complainedofblurvisionforaday.Thevisualacuitywere4/20ODand10/20OS.Thecorrectedvisualacuitywere4/20ODand10/20OS.Theintraocularpressurewere12mmHgODand13mmHgOS.Therewasbloodinthecentralvitreousoftherighteye.Onegiantbreakandthreenailtypebreakswerefoundinthetempleretina.Thetempleretinadetached,yetthemacularareawasnotinvolved(Figure1).Andthefundusofthelefteyewasnormal.Bultrasonicscanshowedtherighteyetempleretinadetachment(Figure2).VEPandERGoftheeyeswerenormal.
简介:目的探讨鼻腔鼻窦鳞状细胞癌侵犯眼眶时保留或剜除眼球对生存率和复发率的影响.方法回顾性分析1989年1月~1998年2月我院收治的43例侵犯眶骨壁和眶骨膜的鼻腔、鼻窦鳞状细胞癌患者的临床和随访资料,其中25例剜除眼球,18例保留眼球.比较患者的3年生存率和复发率.结果随访38~126个月,43例患者中,眼球剜除组3年生存率为56%(14/25),局部复发率为36%(9/25),保留眼球组3年生存率为55.5%(10/18),局部复发率为38.8%(7/18),其中1例为眶内复发.结论经术前或术后放疗,对侵犯眶骨壁和眶骨膜的鼻腔、鼻窦鳞癌,保留眼球组与剜除眼球组相比:有相似的局部复发率和生存率,但保留眼球提高了术后生活质量.
简介:传统的石蜡包埋病理诊断方法由于操作处理所需时间长(一般70~80h),所以不适应现代临床医学快速诊断的要求.
简介:AIM:ToinvestigatetheinterferingeffectofY-27632,aROCK-Iselectiveinhibitor,onthesignaltransductionpathwayoftransforminggrowthfactor-β1(TGF-β1)inocularTenoncapsulefibroblasts(OTFS)invitro.METHODS:AfterOTFSfrompassages4to6invitrowereinducedbyTGF-β1andthentreatedbyY-27632,thechangesoftheOTFScellcycleswereanalyzedviaflowcytometry,andtheproteinsexpressionoftheα-smoothmuscularactin(α-SMA),connectivetissuegrowthfactor(CTGF),collagenIwerecalculatedbyWesternblot.AfterOTFStreatedbythedifferentconcentrationsofY-27632,theexpressionlevelsoftheα-SMA,CTGFandcollagenImRNAwereassayedbyRT-PCR.RESULTS:Y-27632hadnomarkedlyeffectontheOTFScellcycles.AftertreatedbyTGF-β1,OTFSinG1periodsignificantlyincreased.ThecellcyclesdistributionbybothTGF-β1andY-27632hadnoremarkabledifferencefromthatincontrolgroup.Y-27632significantlyinhibitedtheproteinsexpressionsofbothα-SMAandCTGF,whiletosomeextentinhibitedthatofcollagenI.TGF-β1significantlypromotedtheproteinsexpressionsofα-SMA,CTGFandcollagenI.AfterOTFStreatedbybothTGF-β1andY-27632,ofα-SMA,theproteinexpressionwassimilarwiththatincontrolgroup(P=0.066>0.05),buttheproteinexpressionofCTGForcollagenI,respectively,wassignificantlydifferentfromthatincontrolgroup(P=0.000<0.01).Thedifferencesofexpressionsoftheα-SMA,CTGFandcollagenImRNAin30,150,750μmol/LY-27632groupwerestatisticallysignificant,comparedwiththoseincontrolgroup,respectively(α-SMA,P=0.002,0.000,0.000;CTGF,P=0.014,0.002,0.001;collagenI,P=0.003,0.002,0.000).CONCLUSION:BlockingtheRho/ROCKsignalingpathwaybyusingofY-27632couldinhibitthecellularproliferationandtheexpressionofbothCTGFandα-SMAwhateverOTFSinducedbyTGF-β1ornot.Y-27632suppressedtheexpressionofcollagenImRNAwithoutinduction.
简介:目的:探讨裂隙灯眼前段处理系统在眼科临床工作中的各种实际应用状况及操作技巧。方法:应用配置佳能PowerShotA720IS型数码照相机(1200万像素)SLM型裂隙灯显微镜检查眼部病变情况,并在裂隙灯下根据不同的病变位置,在不同色彩、角度下进行照相(放大倍率×10;×16;×20)。结果:采集不同种类疾病具有代表性照片:眼睑及结膜肿物、结膜裂伤、角膜炎、角膜异物、翼状胬肉、前房积血、前房角异物等如图示。结论:裂隙灯眼前段处理系统的应用为临床医疗文献提供直接定性依据,给患者了解自身病情带来便利,照片直观、经济,在眼科领域的临床应用具有广阔的前景。
简介:目的方法结果结论分析青光眼小梁切除术后浅前房的原因及治疗效果。对2003年2月-2008年1月126例(138眼)青光眼小梁切除术后浅前房者(37眼)进行回顾性分析。138眼小梁切除手术有37眼(26.81%)发生浅前房,其发生原因有:滤过过强17眼(45.95%),结膜瓣渗漏14眼(37.84%),脉络膜脱离2眼(5.41%),睫状环阻塞性青光眼1眼(2.7%),其它3眼(8.1%)。其中33眼(89.19%)经保守治疗治愈,4眼(10.81%)经前房注射透明质酸钠治疗,使前房形成。青光眼小梁切除术后浅前房发生率较高,大部分经保守治疗可恢复正常,透明质酸钠在前房重建中效果良好。
简介:<正>DearSir,IamDr.Zhong-ShanChenfromtheDepartmentofOphthalmology,WuhanGeneralHospitalofGuangzhouMilitaryCommand,HubeiProvince,China.Iwritetopresenttwocaseswithserpiginouschoroiditis(SC)whichisarare,usuallybilateral,chronic,progressiveinflammationofthechoroid,choriocapillarisandretinalpigmentepithelium.InthispaperwepresentedthedifferentoutcomesofSCpatientswithorwithoutsystemicandoculartreatmentsafterlong-termfollow-up.
简介:目的总结45例60岁以上老年患者行乙状窦后进路桥小脑角手术的围手术期治疗经验.方法对我科1991年1月至2000年1月行该手术治疗的45例老年患者进行回顾性分析.其中三叉神经痛37例;舌咽神经痛3例;半面痉挛4例;桥小脑角胆脂瘤1例.术前检查异常的有:心电图12例,占26.67%;胸部X线6例,占13.3%;颅脑CT2例,占4.44%,高血压病9例,占20%;糖尿病5例,占11.1%;肝功能异常3例,占6.67%;白细胞低2例,占4.44%;血小板减少2例,占4.44%.结果所有患者均顺利完成手术,术后无严重并发症,均治愈出院,随访2~9年,症状无复发.结论老年患者虽然体质较弱,检查结果异常多,但只要正确处理围手术期的异常,提高手术技巧,老年患者完全能够耐受此类手术并顺利康复.
简介:目的比较小梁切除术后激光断线和可调节缝线两种不同方法调控眼压的疗效。方法回顾性分析2014年12月-2016年5月在郑州大学第一附属医院就诊的原发性慢性闭角型和原发性开角型青光眼患者共47例(52眼),其中男性25例(28眼),女性22例(24眼)。将患者分为两组,A组行小梁切除联合巩膜瓣固定缝线缝合术,B组行小梁切除联合巩膜瓣可调节缝线缝合术,观察术前术后眼压和术后并发症;比较这两种不同方法调控眼压的疗效;结果小梁切除术前A组的眼压为29.5±6.8mmHg,B组眼压为30.5±7.1mmHg,两者比较差异无统计学意义(t=0.74,P〉0.05),术后6个月A组眼压为12.3±5.5mmHg(1kPa=7.5mmHg),B组眼压为13.7±6.3mmHg,两组的差异无统计学意义(t=0.46,P〉0.05)。术后6个月2A组完全成功率为73.1%,B组完全成功率为84.6%,两组比较差异无统计学意义((χ^2=0.31,P〉0.05)。术后浅前房、低眼压和脉络膜脱离的发生率A组较多,但两组比较差异无统计学意义(χ-2=0.15,χ-2=0.02,χ-2=0.02,P〉0.05);结论小梁切除术后激光断线和可调节缝线均能有效的调控术后眼压,减少术后浅前房和低眼压等并发症,获得较理想的术后疗效。
简介:目的对眼科住院医师实施白内障囊外摘除术及人工晶体植入术中,手术并发症的预防及处理能力培训探讨。方法开展防盲治盲工作中,住院医师共施行215例(235只眼)的白内障囊外摘除术及人工晶体植入手术,对发生并发症进行回顾性分析。结果术后发生的手术并发症:后囊破裂15只眼(6.4%),虹膜脱出5只眼(2.1%),虹膜根部离断3只眼(1.3%),角膜水肿45只眼(19.1%),瞳孔上移10只眼(4.3%),前房皮质残留2只眼(0.9%),后囊瞳孔区皮质残留2只眼(0.9%),继发性瞳孔阻滞性青光眼1只眼(0.4%),黄斑囊样水肿1只眼(0.4%)。结论后囊膜破裂和角膜水肿是本组白内障囊外摘除术及人工晶体植入手术主要并发症,住院医师手术者应规范掌握手术要点是预防并发症发生的关键,对出现的手术并发症应积极妥善处理是减少并发症有效措施。