简介：SeveralimportantfactorsaffectingtheefficiencyofAgrobacterium-mediatedricetransformationwerestudiedwithseveralpredominantcommercialindicaandjaponicaricecultivars.Asfarasindicaricecalluswasconcerned,CCmediumwasthebestandthequalityofcalluswasimprovedwiththeadditionof1.0to2.0mg/LABA.Itdecreasedthepercentageofbrowningcalliandimprovedthecallusgrowingstatebyadditionofacertainamountofsorbitoltothesubculturemedium.NBmediumwasthebestforcallusinitiationofjaponicarice,buttheimprovementinthequalityofcallusofjaponicasubspecieswasnotobviousbyaddingABA.Duringtheperiodofsubculture,toacertaindegree,increasingthesucroseconcentrationcouldimprovetheproportionofhygromycinresistantcalli.Furthermore,thetransformationefficiencywouldbehigherbyapplyingselectionpressureintheselectionstage,removingselectionpressureduringtheplantletdifferentiationperiodandapplyingselectionpressureagainduringseedlinghardeningperiod.Besides,suitablecombinationofplanthormoneswasbeneficialforcallusdifferentiation.AnefficientAgrobacterium-mediatedricetransformationsystemhadbeenestablishedforseveralricecultivarsandalotoftransgenicriceplantshadbeenobtained.

简介：ToconstructtheT-DNAinsertionalmutagenesistransformationsystemforricesheathblightpathogenRhizoctoniasolaniAG-1IA,thevirulentisolateGD118ofthispathogenwasselectedasaninitialisolatefortransformation.TheconditionsfortransformationofisolateGD118wereoptimizedinfiveaspects,i.e.pre-inductiontime,co-culturetime,acetosyringone(AS)concentrationattheco-culturephase,co-culturetemperatureandpHvalueofinductionsolidmedium(ISM)attheco-culturephase.Finally,asystem...

简介：