简介：利用PCR以实验室构建的原核重组表达质粒pProEX-OCIF为模板扩增得到N末端融合有6×His标签和rTEV蛋白酶识别序列的人破骨细胞形成抑制因子(OsteoclastogenesisInhibitoryFactor,简称OCIF)结构域D1～D6(简称OCIFm)编码基因片段;将其与pMD18-T连接,转化大肠杆菌TOP10,筛选得到阳性重组质粒pMD18-OCIFm,双酶切重组克隆质粒pMD18-OCIFm得到OCIFm基因片段;将其定向插入甲醇营养型酵母分泌表达载体pPIC9中,构建获得重组表达质粒pPIC9-OCIFm.测序验证后,以限制性内切酶SalⅠ线化,电穿孔转化酵母宿主菌GS115.筛选得到阳性表达菌株后,甲醇诱导表达4d,SDS-PAGE和Westernblot对表达情况进行分析和确认.所获得的OCIFm基因片段在甲醇营养型酵母中表达量占菌体总蛋白的30%以上.利用Ni-NTA树脂对表达产物进行一步亲和层析纯化.活性测定表明纯化的表达产物可诱导体外培养的成熟破骨细胞样细胞的凋亡.表达产物的生物学活性较利用原核表达系统明显提高.
简介：Theground-stateandthermodynamicpropertiesofquantummixed-spinchainsof1/2-1/2-1-1and3/2-3/2-1-1areinvestigatedbyaquantumMonteCarlosimulationwiththeloop-clusteralgorithm.For1/2-1/2-1-1chain,wefindithastwophasesseparatedbyanenergy-gapvanishingpointintheground-state.For3/2-3/2-1-1chain,thenumericalresultsshowtwoenergy-gapvanishingpointsisolatedbydifferentphasesinitsground-state.Ourcalculationsindicatethatallthesegroundstatephasescanbeunderstoodbymeansofvalence-bond-solidpicture,andthethermodynamicbehavioratfinitetemperaturesiscontinuousasafunctionofparameterα=J2/J1.
简介：Inthispaper,thetwo-dimensionalMarcinkewiczintegralintroducedbySteinμ（f）（x）=(∫0x|∫|x-y|≤1|x-y|Ω（x-y）f（y）dy|2t-3dt)2isshowntobeofweaktype（1,1）andweightedweaktype（1,1）withrespecttopowerweight|x|"if-1<α<0,whereΩishomogeneousofdegree0.hasmeanvalue0andbelongstoLlog+L（S1）.
简介：Thereactionof1-phenyl-3-methyl-4-benzoyl-2,5-dihydro-1H-pyrazol-5-one(PMBP)andmethyldithiocarbazate(mdtc)inmethanolresultsinformationofayellowcrystallinesolid,adductof1-phenyl-3-methyl-4benzoyl-2,5-dihydro-lH-pyrazol-5-oneandmethyldithiocarbazate.Whentheyellowsolidsweredissolvedinamixtureofmethanolandether(1:4),aredcrystal,whichisanoxidationproductoftheformer,wasobtainedbyallowingsolventtoevaporateforafewdaysatroomtemperature.TheX-rayanalysisoftheredcrystalindicatesthatitisanoveldisulfidewithaspecialstructurelikea“U”conformationinthesolidstate.
简介：In2014,theresearchersofTheoreticalPhysicsGroupatIMPhavecarriedouttheirresearchworkonnuclearphysics,heavyionphysicsandhadronphysics.Someimportantresultshavebeenobtained.Thethree-bodyforce(TBF)effectontheoff-shellbehavioroftheneutronandprotonmassoperatorsinasymmetricuclearmatterhasbeeninvestigatedwithintheframeworkoftheextendedBrueckner-Hartree-Fockapproach[1].Athighdensitieswellabovethenormalnuclearmatterdensity,theTBFisshowntoaffectsignificantlytheoff-shellehaviorofboththeprotonandneutronmassoperators.Thedensitydependenceofsymmetryenergyaroundtheuclearsaturationdensityhasbeenstudied[2].Anewapproachhasbeenproposedandappliedtodeterminetheymmetryenergycoefficientofheavynucleibasedontheavailableexperimentalnuclearmassesofheavynuclei.Theobtainedresultisadoptedtoanalyzethedensitydependenceofsymmetryenergyofnuclearmatteraroundtheaturationdensity.TheslopeparameteratthesaturationdensityisdeterminedtobeL=(50.515.5)MeV.
简介：α-Aminonitrileswerepreparedefficientlyfromanodiccyanationofα-aminoester.Theeffectofdifferentsolventsandelectrolyteswasstudied.Theotherbyproductswereanalyzed.
简介：Anewspectralproblemisproposed,andnonlineardifferentialequationsofthecorrespondinghierarchyareobtained.Withthehelpofthenonlinearizationapproachofeigenvalueproblems,anewfinite-dimensionalHamiltoniansystemonR2nisobtained.Ageneratingfunctionapproachisintroducedtoprovetheinvolutionofconservedintegralsanditsfunctionalindependence,andtheHamiltonianflowsarestraightenedbyintroducingtheAbel-Jacobicoordinates.Atlast,basedontheprinciplesofalgebracurve,thequasi-periodicsolutionsforthecorrespondingequationsareobtainedbysolvingtheordinarydifferentialequationsandinversingtheAbel-Jacobicoordinates.
简介：分析鉴定ApoCⅢ转基因阳性小型猪.从仔猪尾中提取基因组DNA,通过PCR和Southernblotting等手段进行鉴定;从仔猪肝、小肠、心、肾、肺、脾、胃以及皮肤等冰冻组织以Trizol法提取RNA,以RT-PCR手段进行检测分析.经过对18头仔猪进行PCR分析,显示其中的15头含有人ApoCⅢ基因,随后的测序和Southernblotting亦证实,猪的基因组中已经整合有人ApoCⅢ基因.RT-PCR证实人apoCⅢ基因已经在转基因小型猪的肝脏和小肠中进行了翻译.本研究成功制备了人ApoCⅢ转基因小型猪,可作为高脂血症与动脉粥样硬化疾病之间关系很有价值的研究模型.
简介：随便取一个自然数，如果它是偶数，用２除它；如果它是奇数，将它乘３之后再加１，这样反复运算，最终必然得１．比如，取自然数Ｎ＝６．６是偶数，先用２除，６÷２＝３；３是奇数，要将它乘３之后再加１，３×３＋１＝１０；按着上述法则往下做：１０÷２＝５；５×３＋１＝１６；１６÷２＝８；８÷２＝４；４÷２＝２；２÷２＝１．从６开始经历了３→１０→５→１６→８→４→２→１，最后得１．通过大量演算发现最后结果总是得１．于是数学家提出如下猜想：对于任一个自然数Ｎ，若Ｎ是偶数，就把它变成Ｎ２；若Ｎ是奇数，就把它变成３Ｎ＋１．按照这个规则运算下去，最后必然得１．这个猜想到目前还没有人给予证明，不过日本东京大学的米田信
简介：In2010,theBESCollaboration[1]foundaclearenhancementintheK0K0massdistributionintheJ=ψ!ηK0K0decay.SuchanenhancementisusuallyasignatureofanL=0resonancearoundthreshold,whichinthiscasewouldcorrespondstoanh1statewithquantumnumbersIG(JPC)=0??(1+??).Thish1statewaspredictedbytheChiralUnitarytheoryintheKKinteraction.Becauseoftheconversationlaw,thisstatecanonlydecaytoK0K0channel,andcanbestudiedefficientlyintheJ=ψ!ηK0K0decay.
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![Crystal Structure of Bis{<b style='color:red'>1</b>-methylthio-<b style='color:red'>1</b>-[<b style='color:red'>1</b>-phenyl-<b style='color:red'>1</b>-（<b style='color:red'>1</b>-phenyl3-methyl-5-oxo-4,5-dihydropyrazol-4-ylene）-methylamino]iminomethyl} Disulfide with the “U” Conformation](/image/thesis10 "Crystal Structure of Bis{<b style='color:red'>1</b>-methylthio-<b style='color:red'>1</b>-[<b style='color:red'>1</b>-phenyl-<b style='color:red'>1</b>-（<b style='color:red'>1</b>-phenyl3-methyl-5-oxo-4,5-dihydropyrazol-4-ylene）-methylamino]iminomethyl} Disulfide with the “U” Conformation")
![方程x＋<b style='color:red'>1</b>=xnexp[rn<b style='color:red'>1</b>—xn／<b style='color:red'>1</b>—λx^n]的全局吸引性](/image/thesis16 "方程x＋<b style='color:red'>1</b>=xnexp[rn<b style='color:red'>1</b>—xn／<b style='color:red'>1</b>—λx^n]的全局吸引性")
