简介:Thetitlecompounds4,4'-oxobis(propham)((?)n)(C20H24N2O5,Mr.=372.41)wassynthesizedbytheadditionreactionofcorresponding4,4'-oxobisphenyldiisocyanatewithisopropanol.ThecrystalstructurewasdeterminedbyX-raydiffractionmethod.Thecrystalistriclinicsystem,spacegroupP-1witha=8.5107(17),b=9.1164(18),c=14.5701(3)A,α=80.44(3),β=85.25(3),γ=62.88(3)°,V=992.2(3)A3,Z=2,Dc=1.247g/cm3,F(000)=396,R=0.0520,wR2=0.1434for4336observedreflectionswith[I>2σ(I)].X-rayana...
简介:本研究以双单倍体马铃薯DM的突变体为材料,利用PCR-walking的方法扩增其突变体的侧翼序列。在试验中,以60个鉴定过的阳性突变体样品为材料扩增后,有28个样品扩增出有效条带。PCR胶回收后测序,有2个样品测序无信号,其他26个样品,将测序与载体序列和马铃薯基因组序列用BLASTN比对,发现有6个样品分别插入到了马铃薯1、3或7号染色体上。通过进一步比对,发现编号为TDM90的突变体中,载体插入了PREDICTED:Solanumtuberosumtrans-resveratroldi-O-methyltransferase-like(LOC102590422),mRNA马铃薯反式白藜芦醇基因中。试验说明了PCR-walking法扩增马铃薯侧翼序列是可行的。且载体插入马铃薯反式白藜芦醇基因中对马铃薯白藜芦醇基因的表达有何影响,有进一步研究下去的重要意义。
简介:Inordertoexplorethefeasibilityofcryopreservationondormantforesttreeseeds,theseedviabilityof4species(Malusbaccata,Prunusdavidiana,P.armenicaandAmygdalupersica)withmoisturecontent(MC)changingwastestedafter30-daystorageinliquidnitrogenof-196℃.Theresultsshowedthatallthe4speciesofseedscankeepastableviabilityaftercryopreservation.Intheprocedure,theoptimumMCwas48g/kgforM.baccata,27g/kgforP.davidiana,31g/kgforP.armenicaand45g/kgforA.persica,...