简介：AIM:Toinvestigatethebiologicaleffectsofinternalirradiation,andthetherapeuticeffectivenesswasassessedof131I-labeledanti-epidermalgrowthfactorreceptor（EGFR）liposomes,derivedfromcetuximab,whenusedasatumor-targetingcarrierinacolorectalcancermousemodel.METHODS:WedescribedtheliposomesandcharacterizedtheirEGFR-targetedbindingandcellularuptakeinEGFR-overexpressingLS180colorectalcancercells.Afterintra-tumorinjectionsof74MBq（740MBq/mL）131I-antiEGFR-BSA-PCL,weinvestigatedthebiologicaleffectsofinternalirradiationandthetherapeuticefficacyof131I-antiEGFR-BSA-PCLoncolorectalcancerinamaleBALB/cmousemodel.Tumorsize,bodyweight,histopathology,andSPECTimagingweremonitoredfor33dpost-therapy.RESULTS:Therapidradioiodineuptakeof131I-antiEGFR-BSA-PCLand131I-BSA-PCLreachedmaximumlevelsat4hafterincubation,andthe131Iuptakeof131I-antiEGFR-BSA-PCLwashigherthanthatof131I-BSAPCLinvitro.The131Itissuedistributionassayrevealedthat131I-antiEGFR-BSA-PCLwasmarkedlytakenupbythetumor.Furthermore,atissuedistributionassayrevealedthat131I-antiEGFR-BSA-PCLwasmarkedlytakenupbythetumorandreacheditsmaximaluptakevalueof21.0±1.01%ID/g（%ID/gisthepercentageinjecteddosepergramoftissue）at72hfollowingtherapy;thedrugconcentrationinthetumorwashigherthanthatintheliver,heart,colon,orspleen.Tumorsizemeasurementsshowedthattumordevelopmentwassignificantlyinhibitedbytreatmentswith131I-antiEGFR-BSA-PCLand131I-BSA-PCL.Thevolumeoftumorincreased,andtreatmentratewith131I-antiEGFR-BSA-PCLwas124%±7%,lowerthanthatwith131I-BSA-PCL（127%±9%）,131I（143%±7%）,andnormalsaline（146%±10%）.Thepercentagelossesinoriginalbodyweightswere39%±3%,41%±4%,49%±5%,and55%±13%,respectively.Thebestsurvivalandcurerateswereobtainedinthegrouptreatedwith131I-antiEGFR-BSA-PCL.Theanimalsinjec

简介：

简介：随着我国考试竞争压力的逐渐增大,中小学体育学科课程占到总课程的5~10%左右,中小学的实践体育课程越来越少,再加上各种培训体育课程的负担,“高分低能”现象越来越严重。BSA(BoyScoutsofAmerica)美国童子军,自1910年成立起,就明确了其目标是与现有教育不同,着重强调的是青少年儿童为自己和别人做事的能力。本文选取河南6所中小学为研究对象综合运用文献综述法、问卷调查法、分析法等研究方法,通过对美国童子军体育课程体系的分析和研究,探索将美国童子军体育课程融入中小学教学的可行性和必要性,望对该省的教育有所启示。

简介：牛血清白蛋白（BSA）在一定浓度范围内与其荧光强度以及位于280nm处的吸光度呈线性相关;中药白头翁和半枝莲的水提取液自身在BSA荧光发射峰位置的荧光强度比较弱,但在BSA的紫外吸收区域吸收峰范围广泛且吸收值很强;两种中药中含有某些可使BSA荧光猝灭的物质,且当中药与BSA共存时,体系的紫外吸收光谱也发生了显著的变化,表明两种中药与BSA发生了相互作用,且这种作用是在分子水平上的.

简介：