简介:运用PCR技术扩增吉富罗非鱼选育群体第20和21世代D-loop区799bp序列并进行群体遗传变异分析.序列组成分析显示两代群体的A和T含量较为接近,A+T含量明显高于G+C含量.两代群体的D-loop序列中度变异,群体间遗传变异较小,遗传分化主要发生在群体内.中性检验(Tajima’sD和FuandLi’sD)分析结果显示两群体不遵循中性进化模型,以中等频率的等位基因为主.基于D-loop序列进行的遗传多样性分析显示,两代群体的多态性位点、平均核苷酸差异数、单倍型多样性、核苷酸多样性等参数较大,且两代群体属于高单倍型多样性和高核苷酸多样性的遗传多样性模式.本研究为吉富罗非鱼的分子标记选育提供参考依据.
简介:根据mtDNAD-loop基因对钦州茅尾海尖鳍鲤群体(n=34)的遗传多样性进行了研究.通过PCR技术进行了mtDNAD-loop基因扩增,检测到变异位点共计18个,占全部的1.97%.其中:3个是颠换位点,14个是转换位点(C-T,A-G),1个转换位点与颠换位点同时存在.34尾个体分属14种单倍型.尖鳍鲤线粒体DNA(mtDNA)核苷酸多样性(π)为0.00301,平均核苷酸差异数(K)为2.747.单倍型多样度(H)为0.911,单倍型间平均遗传距离(P)为0.004.用单倍型间遗传距离构建的NJ系统树由4个支系组成.尖鳍鲤茅尾海种群线粒体(mtDNA)D-loop基因多态性较低,表明该种群遗传多样性相对较低,因此,保护尖鳍鲤种质资源刻不容缓.
简介:用线粒体DNA的D-loop和Cytb基因序列分析方法研究了吉林延吉、敦化和辽宁法台3个区域的29尾拉氏PhoxinuslagowskiiDybowsky的遗传多样性。经PCR扩增和测序,获得了783~785bpD-loop和818bpCytb的同源序列。两者多态性遗传参数统计显示,29尾个体分别存在47(D-loop)和89(Cytb)个变异位点,分别检测出15(D-loop)和11(Cytb)个单倍型,总群体单倍型(H_d)分别为0.8966(D-loop)和0.8990(Cytb),核苷酸多样性指数(Pi)分别为0.0246(D-loop)和0.0498(Cytb),平均核苷酸差异数(K)分别为19.2857(D-loop)和40.7365(Cytb)。分子方差分析(AMOVA)结果表明,79.02%(D-loop)和81.69%(Cytb)变异来自群体间,20.98%(D-loop)和18.31%(Cytb)来自群体内。单倍型呈明显的地理差异,分成2个分支,一个以延吉群体为主,一个以法台群体为主。拉氏的遗传多样性水平较高,群体间遗传分化明显。该结果可为拉氏的种质资源保护提供参考。
简介:ThepresentstatusofPPtechnologyinChinaispresented.ThroughareviewofsingleloopanddualloopPPreactortechnologyaswellasdevelopmentandutilizationofPPproductsitisconcludedthatimportofPPtechnologyshouldbebasedonthedemandofPPproductsandmarkettrendwithconsiderationoflocalconditions.TheexistingproblemsandfuturedevelopmentofPPtechnologyinChinaarediscussed.
简介:分布在黑龙江上游呼玛河和额木尔河等水域的北极茴鱼一新纪录种Thymallussp,种群数量少、分布范围狭小,群体资源面临濒危。本研究采用PCR技术分析了黑龙江上游的北极茴鱼线粒体DNA控制区的序列,共获得了1104bp核苷酸全序列,与分布于勒拿河的该种茴鱼群体进行比较。结果显示:在23尾个体中共检测到3个单倍型(Hap1、Hap2,及Hap3),其中Hap3为黑龙江和勒拿河共享;利用单倍型构建的分子系统树和网络图显示,黑龙江群体可能起源于勒拿河。黑龙江上游群体的遗传多样性较低,单倍型多样性指数(0.530)和核苷酸多样性(0.00053)远低于同域分布的其它种茴鱼;黑龙江群体与勒拿河群体的Fst为38.56%(P〈0.05),已产生一定的遗传分化。
简介:Inthispaper,adiscrete-timeanalysisofthethird-ordercharge-pumpbasedphase-lockedloops(CPLLs)ispresentedinthepresenceofloopdelay.Thez-domainanalysisoftheclosed-looptransferfunctionisderivedandcomparedwiththetraditionals-domainmethod.ThesimulationresultsunderSPECTREshowthat,duetothesamplingnatureofCPLL,thetraditionals-domainanalysisisunabletopredictitsjitterpeakingaccurately,especiallywhentheloopdelayistakenintoconsideration.Theimpactofloopdelayonthestabilityofthethird-orderCPLLsystemisfurtheranalyzedbasedontheproposedway.ThestabilitylimitofthewidebandwidthCPLLwithloopdelayiscalculated.Thecircuitsimulationresultsagreewellwithmathematicalanalysis.
简介:Overthepasttwodecadesconsiderableprogresshasbeenmadeinunderstandingtheototoxiceffectsandmechanismsunderlyingloopdiuretics.Astypicalrepresentativeofloopdiureticsethacrynicacidorfurosemideonlyinducestemporaryhearingloss,butrarelypermanentdeafnessunlessappliedinsevereacuteorchronicrenalfailureorwithotherototoxicdrugs.Loopdiureticinduceuniquepathologicalchangesinthecochleasuchasformationofedematousspacesintheepitheliumofthestriavascularis,whichleadstorapiddecreaseoftheendolymphaticpotentialandeventuallossofthecochlearmicrophonicpotential,summatingpotential,andcompoundactionpotential.LoopdiureticsinterferewithstrialadenylatecyclaseandNat/Kt-ATPaseandinhibittheNa-K-2Clcotransporterinthestriavascularis,howeverrecentreportsindicatethatoneoftheearliesteffectsinvivoistoabolishbloodflowinthevesselssupplyingthelateralwall.Sinceethacrynicaciddoesnotdamagethestriavascularisinvitro,thechangesinNat/Kt-ATPaseandNa-K-2Clseeninvivomaybesecondaryeffectsresultsfromstrialischemiaandanoxia.Recentobservationsshowingthatreninispresentinpericytessurroundingstriaarteriolessuggestthatdiureticsmayinducelocalvasoconstrictionbyreninsecretionandangiotensinformation.Thetightjunctionsintheblood-cochleabarrierpreventtoxicmoleculesandpathogensfromenteringcochlea,butwhendiureticsinduceatransientischemia,thebarrieristemporarilydisruptedallowingtheentryoftoxicchemicalsorpathogens.
简介:Inthispaper,anewlooptransformationisproposedthatcanexpolitparalleisminloopswhichcannotbefoundbytraditionalmethods.Thenthemethodisextendedtoshowhowtoachievemaximumspeedupofloopsifthereareinfiniteprocessorsandhowtobalancetheworkloadofparallelsectionsinloopsifthereisfixednumberofprocessors.
简介:At-speedtestingusingexternaltesterrequiresanexpensiveequipment,thusbuilt-inself-test(BIST)isanalternativetechniqueduetoitsabilitytoperformon-chipat-speedself-testing.ThemainissueinBISTforat-speedtestingistoobtainhighdelayfaultcoveragewithalowhardwareoverhead.Thispaperpresentsanimprovedloop-basedBISTscheme,inwhichaconfigurableMISR(multiple-inputsignatureregister)isusedtogeneratetest-pairsequences.ThestructureandoperationmodesoftheBISTschemearedescribed.Thetopologicalpropertiesofthestate-transition-graphoftheproposedBISTschemeareanalyzed.Basedonit,anapproachtodesignandefficientlyimplementtheproposedBISTschemeisdeveloped.ExperimentalresultsonacademicbenchmarkcircuitsarepresentedtodemonstratetheeffectivenessoftheproposedBISTschemeaswellasthedesignapproach.
简介:与exascale来超级计算的时代,电源效率成为了最重要的障碍造一个exascale系统。Dataflow建筑学在为科学应用完成高电源效率有本国的优点。然而,最先进的dataflow体系结构没能为循环处理利用高并行。处理这个问题,我们建议一个pipelining环优化方法(PLO),它在处理元素(PE)在环流动做重复dataflow的数组加速器。这个方法由二种技术,帮助建筑学的硬件重复和帮助说明的软件重复组成。在硬件重复执行模型,一个在薄片上循环控制器被设计产生循环索引,减少计算内核并且打为pipelining执行的一个好基础的复杂性。在软件重复实行模型,另外的环指令被论述解决重复相关性问题。经由这二种技术,准备好了每周期执行的指令的平均数字被增加使浮点联合起来忙。当这二种技术的硬件费用是可接受的时,模拟结果证明分别地,我们的建议方法平均由2.45x和1.1x在浮点效率超过静电干扰和动态循环执行模型。
简介:AnewmethodforconstructinginterpolatingLoopsubdivisionsurfacesispresented.ThenewmethodisanextensionoftheprogressiveinterpolationtechniqueforB-splines.GivenatriangularmeshM,theideaistoiterativelyupgradetheverticesofMtogenerateanewcontrolmeshMsuchthatlimitsurfaceofMwouldinterpolateM.ItcanbeshownthattheiterativeprocessisconvergentforLoopsubdivisionsurfaces.Hence,themethodiswell-defined.Thenewmethodhastheadvantagesofbothalocalmethodandaglobalmethod,i.e.,itcanhandlemeshesofanysizeandanytopologywhilegeneratingsmoothinterpolatingsubdivisionsurfacesthatfaithfullyresembletheshapeofthegivenmeshes.Themeshesconsideredherecanbeopenorclosed.
简介:ThispaperintroducestherecentappearanceofacirculareconomyinsuchindustrializedcountriesasGermanyandJapanandoutlinesitsconceptandprinciples.Theauthoremphasizesthatacirculareconomyshouldbecomethebreakthroughpointforsustainabledevelopmentandwillenableashifttoasustainablesocietyof'doinglesswithless'awayfromthecurrenthigh-consumptionsocietyof'doingmorewithmore'
简介:<正>Inthispaper,wemakeacompletestudyoftheunfoldingofaquadraticintegrablesystemwithahomoclinicloop.MakingaPoincaretransformationandusingsomenewtechniquestoestimatethenumberofzerosofAbelianintegrals,weobtainthecompletebifurcationdiagramandallphaseportraitsofsystemscorrespondingtodifferentregionsintheparameterspace.Inparticular,weprovethattwoisthemaximalnumberoflimitcyclesbifurcatingfromthesystemunderquadraticnon-conservativeperturbations.
简介:Adistibutedoptimallocaldoubleloop(DOLDL)networkispresented.EmphasisislaidonthetopologyanddistributedroutingalgorithmsfortheDOLDL.Onthebasisofbuildinganabstractmodel,asetofdefinitionsandtheoremsaredescribedandproved.Analgorithmwhichcanoptimizethedoubleloopnetworksispresented.TheoptimalvaluesofthetopologicparametersfortheDOLDLhavebeenobtainedbythealgorithm,andthesenumer.calresultsareanalyzed,Thestudyshowsthattheboundsoftheoptimaldiameter(d)andaveragehopdistance(a)forthisclassofnetworksareand,respectively(Nisthenumberofnodesinthenetwork.「3≤N≤10~4).AclassofthedistributedroutingalgorithmsfortheDOLDLandtheimplementationprocedureofanadaptivefault-tolerantalgorithmareproposed.Thecorrectnessofthealgorithmhasbeenalsoverifiedbysimulating.
简介:Thisstudydefinesandassessestheselectionciiteriaforsuitableparticulatematerialstobeusedinanupflowbubblingfluidizedbed(UBFB)ordenseup-flowpowdercirculationsystemforsolarenergycaptureandstorage.Themaincriteriaidentifiedarebasedonthethermophysicalandthermomechanicalproperties,attritionbehavior,andtheconsiderationsofhealthandenvironmentalhazardsofthecandidatepowders.Finally,acostcomparisonandtentativerankingofthedifferentcandidatepowdersispresentedinadditiontoaweightedscoring.Significantscoringdifferencescanbeobservedbetweenthevariousmaterials.Olivinepossessesthemostfavorablecharacteristicsandappearstobetheparticulatematerialofchoiceforsolid/gassuspensionheattransferfluids.