简介：Vibrationofacircularmembraneincontactwithafluidhasextensiveapplicationsinindustry.Thenaturalvibrationfrequenciesfortheasymmetricfreevibrationofacircularmembraneincontactwithaboundedincompressiblefluidarederivedinthispaper.Consideringsmalloscillationsinducedbythemembranevibrationinanincompressibleandinviscidfluid,thevelocitypotentialfunctionisusedtodescribethefluidfield.Twoapproachesareusedtoderivethefreevibrationfrequenciesofthesystem,whichincludeavariationalformulationandanapproximatesolutionemployingtheRayleighquotientmethod.Agoodcorrelationisfoundbetweenfreevibrationfrequenciesevaluatedbythesemethods.Finally,theeffectsofthefluiddepth,themassdensity,andtheradialtensiononthefreevibrationfrequenciesofthecoupledsystemareinvestigated.

简介：Aneigenvaluemethodconsideringthemembranevibrationofwrinklingout-of-planedeformationisintroduced,andthestressdistributingruleinmembranewrinkledareaisanalyzed.Adynamicanalyticalmodelofrectangularshearwrinkledmembraneanditsnumericalanalysisapproacharealsodeveloped.Resultsindicatethatthestressinwrinkledareaisnotuniform,i.e.itislargerinwrinklingwavepeaksalongwrinklesandtwoendsofwrinkleinverticaldirection.Vibrationmodesofwrinkledmembranearestronglycorrelatedwiththewrinklingconfigurations.Therigidityislargerduetotheheavierstressinthepartofwrinklingwavepeaks.Therefore,wavepeaksarealwayslocatedatthenodelinesofvibrationmode.Thevibrationfrequencyobviouslyincreaseswiththevibrationofwavepeaks.

简介：Compositediaphragminflation(CDI)experimentisausefulmethodologyforexploringthemechanicsrelationofcell-celljunctionswithinalivingepithelialsheet.Inthispaper,anewmethodologyofCDIexperimenthasbeenputforward.Thefinitedeformationofacircularpolydimezhylsiloxaneelastomericmembrane,whichisusedasthesubstrateforthecultureofepithelialcells,underhydrostaticpressureisexploredbythetheoryofnon-linearcontinuummechanics.Thesimplifiedgoverningequationswithminimumamountofdifferentialequationsandboundaryconditionsareobtainedandsolvedbymultipleshootingmethod.Theinfluencesonthemembranebyhydrostaticpressurehavebeendiscussed.

简介：Waterisanimportantcomponentinplantcellswithplantaquaporinbeingthemajorproteinforwatertransportinandbetweenplantcells.Asasubfamilyofplantaquaporins,theplasmamembraneintrinsicproteins（PIPs）locatedintheplasmamembraneareclassic,highwater,selectivechannelproteins.ThispaperfocusesonrecentadvancesinthemolecularbiologyofPIPsconcerningstructuralcharacteristics,biologicalfunction,andaregulationmechanism.PIPspossesstwohighlyconserveddomains:GGGANXXXXGYandTGI/TNPARSL/FGAAI/VI/VFWF/YN.PIPscanalsobedividedintotwophylogeneticsubgroupsnamedPIP1andPIP2.PIP1possesseslongerNterminalsequencesandshorterCterminalsequencesthanPIP2withconservedaminoacidsequencesrespectively.StudiesoftransgenicplantsandexpressioninXenopusoocytescellsindicatethatPIPsnotonlymayfacilitatetransportofwaterandsmallneutralsoluteslikeCO2andglycerin,buttheyalsopossessmanyphysiologicalfunctions.Thefunctionsofplantaquaporinsareregulatedbymanyfactorsincludingpost-translationalmodification,heteromerization,pHvalue,anddivalentcations.TheseresultsindicatedthatPIPsactasapivotalroleinwaterandsmallneutralsolutestransportinplants.

简介：ObjectiveTheaimofthisstudyistoinvestigatetheeffectoftransfectedhTERTgeneoncellapoptosisofnewbornratcochlearbasilarmembranecells(CBMCs).MethodsCBMCsisolatedfromnewbornratcochlearweretransfectedusingaplasmidcontaininghumantelomerasereverasetranscriptasegene(pCI-neo-hTERT),andwerescreenedusingG418toobtainstabletransfectedcelllines.Cellapoptosisratewasanalyzedbyflowcytometry.hTERTandapoptosisrelatedgenesexpressionweredetectedbyreversetranscriptasepolymerasechainreaction(RT-PCR).ResultshTERTgeneexpressionwasdetected72hoursaftergenetransfectionintransfectedcells.TheapoptoticrateoftransfectedCBMCssignificantlyreduced.Expressionofapoptosisrelatedgenescorrespondinglychanged.ConclusionTransfectionofhTERTgeneleadstoreducedapoptosisrateinnewbornratCBMCs.andlowerexpressionofapaf1,Caspase3andBCL2intransfectedcellsascomparedtothatofnormalCBMCs.

简介：白手起家的断然控告了nanofiltration(NF)膜被用来对待纺织品染料自河为影响象操作压力那样的nanofiltration过程的复用，和因素产生水，喂fl嗷并且膜清洗被调查。与1.0MPa和40L/h的feedflow的一个应用压力，这NF膜在完成100%的浓度移动的TDS，咸度和电导率为CODcr和约51.0%的减小有93.3%的移动。通过这膜获得的permeate对再循环合适。而且，膜能在与1%NaOH答案被清洗以后被再使用。

简介：阳离子exchangers(CAX)属于广泛地在最后十年在植物tonoplasts被调查了的cation/Ca2+exchanger总科。最近，在植物涉及重金属累积和忍耐的CAX的角色为植物救治和食物安全被学习了。在这微型评论，我们在Ca2+信号transduction总结Ca2+/H+antiporter的角色，维持离子动态平衡并且扣押重金属进液泡。而且，我们在场在重金属detoxification的血浆膜Ca2+/H+antiporter的一个可能的角色。