简介：

简介：Objective:Oxidativestressislinkedtoincreasedriskofgastriccancerandmatrixmetalloproteinases(MMPs)areimportantintheinvasionandmetastasisofgastriccancer.WeaimedtoanalyzetheeffectoftheaccumulationofoxidativestressinthegastriccancerMKN-45and23132/87cellsfollowinghydrogenperoxide(H2O2)exposureontheexpressionpatternsofMMP-1,MMP-3,MMP-7,MMP-9,MMP-10,MMP-11,MMP-12,MMP-14,MMP-15,MMP-17,MMP-23,MMP-28,andβ-cateningenes.Methods:ThemRNAtranscriptsinthecellsweredeterminedbyRT-PCR.FollowingH2O2exposure,oxidativestressintheviablecellswasanalyzedby2’,7’-dichlorofluoresceindiacetate(DCFH-DA).Caffeicacidphenethylester(CAPE)wasusedtoeliminateoxidativestressandtheconsequenceofH2O2exposureanditsremovalontheexpressionsofthegeneswereevaluatedbyquantitativereal-timePCR.Results:TheexpressionsofMMP-1,MMP-7,MMP-14,MMP-15,MMP-17andβ-catenininMKN-45cellsandonlytheexpressionofMMP-15in23132/87cellswereincreased.RemovaloftheoxidativestressresultedindecreaseintheexpressionsofMMPgenesofwhichtheexpressionswereincreasedafterH2O2exposure.β-catenin,atranscriptionfactorformanygenesincludingMMPs,alsodisplayeddecreasedlevelsofexpressioninbothofthecelllinesfollowingCAPEtreatment.Conclusions:OurdatasuggestthatthereisaremarkablelinkbetweentheaccumulationofoxidativestressandtheincreasedexpressionsofMMPgenesinthegastriccancercellsandMMPsshouldbeconsideredaspotentialtargetsoftherapyingastriccancersduetoitscontinuousexposuretooxidativestress.

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简介：胆碱是在精子膜结构和流动性的规定的一个关键因素，并且这营养素在成熟和精子的使肥沃的能力起一个重要作用。phosphatidylethanolamineN-methyltransferase(PEMT)和胆碱脱氢酶(CHDH)的抄本，胆碱新陈代谢的二基本的酶，在人的睾丸被观察了，在这纸巾表明他们的基因表示。在现在的学习，我们探索了PEMT和CHDH基因变体的贡献到精子参数。200oligospermic和250个normozoospermic人被招募。DNA从精子，和PEMT−被提取；774G>；C和CHDH+；432G>；T多型性是genotyped。PEMT−的遗传型分发；774G>；C多型性没在oligospermic和normozoospermic人之间不同。相反，在CHDH+的情况中；432G>；T多型性，oligospermic人比normozoospermic人更经常介绍了CHDH432G/G遗传型(62%；对42%；，P<；0.001)。PEMT774G/G遗传型在oligospermic人与PEMT774G/C和774C/C遗传型相比与更高的精子集中被联系(12.5±；5.6×；106精子ml−1对8.3±；5.2×；106精子ml−1,P<；0.002)并且normozoospermic人(81.5±；55.6×；106对68.1±；44.5×；106精子ml−1,P<；0.006)。另外，CHDH432G/G遗传型在oligospermic与CHDH432G/T和432T/T遗传型相比与更高的精子集中被联系(11.8±；5.1×；106对7.8±；5.3×；106精子ml−1,P<；0.003)并且normozoospermic人(98.6±；62.2×；106对58.8±；33.6×；106精子ml−1,P<；0.001)。在我们的系列，PEMT−；774G>；C和CHDH+�

简介：GA-hardnessandinterdependencebetweengenesinthechromosomeareimportantquestionsinthestudyofgeneticalgorithms(GA).Traditionalmethods,whichareusedtomeasuretheinteractionbetweengenes,canonlyreflecttheextentofepistasisbetweenallgenesinthechromosome.Therefore,thedefinitionofthefitnesslandscapeofschemataisproposedinthispaper,andepistasismeasuresonthislandscapeofschemataareusedtoanalyzethedegreeofinterdependencebetweensomecertaingenelociinstudy.Someinformationbetweenthesesitescanbereflectedbysomecharactersofthefitnesslandscapeofschematawhicharecomposedofthesefixedsites.Thestrongertheinteractionbetweenthesesites,thelargerthevariationofthefitnessofschematawhosefixedsitescorrespondtothosesitesinstudy,andthemoreruggedthefitnesslandscapeoftheseschemata.Accordingtothedegreeofinteractionbetweenthesegivengeneloci,buildingblocksofGAcanbeanalyzedanddetermined,andfurthergeneticoperatorsandthestructureofGAcanbedesignedandadjustedtoimprovetheperformanceofGA.Atlast,alotofexperimentsincludingNK-modelsaredone,andresultsofempiricalanalysisshowthatthismethodiseffective.

简介：一个实验被进行用荧光灯的微分显示器(软式磁碟机)方法在干旱应激和正常条件下面在米饭叶子和根比较信使rna表达式差别。一积极碎片被H.A的联合孤立黄页(contained0.1%H.A。黄)分离和宏数组屏蔽方法。比作ArabidopsisthalianaNADPH氧化还原酶基因，它有96%身份。cDNA是1423bp，并且包含了与345氨基酸残余编码蛋白质的1048bp的一个完全的开的读物框架。而且，基因表示水平在正常条件下面比那在干旱应激下面是更高的。在干旱反应下面的NADPH氧化还原酶基因的可能的角色也被讨论。

简介：Themajorstoragesubstanceinriceendospermisstarch,whichaccountsfor80%ofdrymatterweight.Inthisstudy,ricemutantflo7,selectedfromtheprogenyofNipponbare’stissueculture,displayedflouryandopaqueendosperm.Comparedwithitscorrespondingwildtype(WT)Nipponbare,themutantflo7producedlonger,narrower,thinnerandlightergrains.Thelevelsofglucose,fructoseandsucroseinthemutantflo7endospermwerehigherthanthoseintheWTendosperm,whereastheproteincontentwasnotaffected.Withrespecttobothamylosecontentandgelconsistency,themutantflo7waslowerthanWT,butitsalkalivaluewashigher.Scanningelectronmicroscopicexaminationsshowedthattheendospermofthemutantflo7containedirregular,looselypackedandcompoundstarchgranules.Geneticanalysisindicatedthatthemutantphenotypewasdeterminedbyasinglerecessivenucleargene.Theflo7locuswasmappedtoaregiononthelongarmofchromosome12,withina95.1kbintervaldefinedbythemarkersC2-11andC5-15.Thereare13openreadingframesinthemappinginterval.Transcriptionprofilingofthedevelopinggrainsshowedthatanumberofgenesinvolvedinstarchsynthesiswereaffecteddifferentlyinthemutantflo7.

简介：抽象Trehalose在保护有机体免受各种各样的压力的伤害起一个重要作用。Trehalose-6-phosphatesynthase(TPS)是在trehalose合成的关键酶，但是在昆虫，一些仅仅TPS基因被识别了，他们的功能很好没被描绘。更好在昆虫理解TPS的功能，完全的TPS互补DNA(cDNA)克隆从蝗虫草之小穗migratoriamanilensis的胖身体被获得(GenBank就职数字：EU131894)。全身的cDNA是2806bp，包括2442bp的一个开的读物框架，它与91976道尔顿的计算分子的重量和6.14的一个等电位的点编码813氨基酸蛋白质。推出的氨基酸顺序高度类似于另外的出版昆虫TPS，它的C终端也有对trehalose磷酸盐phsophatase(TPP)相应的一个区域。半量的分析显示TPS抄本不仅在胖身体，而且在内脏，血淋巴和腿肌肉被表示。这些数据可以在昆虫便于TPS功能的研究并且改进我们trehalose新陈代谢的理解。

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简介：Predictingprotein-codinggenesstillremainsasignificantchallenge.Althoughavarietyofcomputationalprogramsthatusecommonlymachinelearningmethodshaveemerged,theaccuracyofpredictionsremainsalowlevelwhenimplementinginlargegenomicsequences.Moreover,computationalgenefindinginnewlyse-quencedgenomesisespeciallyadifficulttaskduetotheabsenceofatrainingsetofabundantvalidatedgenes.Herewepresentanewgene-findingprogram,SCGPred,toimprovetheaccuracyofpredictionbycombiningmultiplesourcesofevidence.SCGPredcanperformbothsupervisedmethodinpreviouslywell-studiedgenomesandunsupervisedoneinnovelgenomes.BytestingwithdatasetscomposedoflargeDNAsequencesfromhumanandanovelgenomeofUstilagomaydi,SCG-Predgainsasignificantimprovementincomparisontothepopularabinitiogenepredictors.WealsodemonstratethatSCGPredcansignificantlyimprovepredic-tioninnovelgenomesbycombiningseveralforeigngenefinderswithsimilarityalignments,whichissuperiortootherunsupervisedmethods.Therefore,SCG-Predcanserveasanalternativegene-findingtoolfornewlysequencedeukaryoticgenomes.Theprogramisfreelyavailableathttp://bio.scu.edu.cn/SCGPred/.

简介：Folliclestimulatinghormoneβ(FSHβ)ofJapaneseflounder(Paralichthysolivaceus)playsakeyroleintheregulationofgonadaldevelopment.ThisstudyaimedtoinvestigatemoleculargeneticcharacteristicsoftheFSHβgeneandelucidatetheeffectsofsinglenucleotidepolymorphisms(SNPs)ofFSHβonreproductivetraitsinJapaneseflounder.Weusedpolymerasechainreactionsingle-strandconformationpolymorphism(PCR-SSCP)andsequencingoftheFSHβgenein60individuals.WeidentifiedonlyanSNP(T/C)inthecodingregionofexon3ofFSHβ.TheSNP(T/C)didnotleadtoaminoacidchangesattheposition340bpofFSHβgene.StatisticalanalysisshowedthattheSNPwassignificantlyassociatedwithtestosterone(T)levelandgonadosomaticindex(GSI)(P<0.05).IndividualswithgenotypeTCoftheSNPhadsignificantlyhigherserumTlevelsandGSI(P<0.05)thanthatofgenotypeCC.Therefore,FSHβgenecouldbeausefulmolecularmarkerinselectionforprominentreproductivetraitinJapaneseFlounder.

简介：Interleukin-4isacytokineproducedbyactivatedTcells,mastcells,andbasophilsthatelicitsmanyimportantbiologicalresponses[1](seeTab1).TheseresponsesrangefromtheregulationofhelperTcelldifferentiation[2]andtheproductionofIgE[3]totheregulationoftheadhesivepropertiesofendothelialcellsviaVCAM-1[4],Inkeepingwiththesediversebiologicaleffects,high-affinitybindingsitesforIL-4(Kd20to300pM)havebeendetectedonmanyhematopoieticandnon-hematopoieticcelltypesatlevelsrangingfrom50to5000sitespercell[5].ThisreviewwillfocusonthediscretesignaltransductionpathwaysactivatedbytheIL-4recxeptorandthecoordinationoftheseindividualpathwaysintheregulationofafinalbiologicaloutcome.

简介：·NSGeodeGX1—300处理器3．5寸嵌入式主板，配有LCD，LVDS，视频输出，以太网，音频，CompactFlash，和USB口。

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简介：Emodin(1,3,8-trihydroxy-6-methylanthraquinone)couldenhancethesensitivityoftumorcellstoarsenictrioxide(As2O3)-inducedapoptosisviagenerationofROS,butthemolecularmechanismhasnotbeenelucidated.Here,wecarriedoutcDNAmicroarray-basedglobaltranscriptionprofilingofHeLacellsinresponsetoAs2O3/emodincotreatment,comparingwithAs2O3-onlytreatment.Theresultsshowedthattheexpressionofanumberofgeneswassubstantiallyalteredattwotimepoints.Thesegenesareinvolvedindifferentaspectsofcellfunction.Inadditiontoredoxregulationandapoptosis,ROSaffectgenesencodingproteinsassociatedwithcellsignaling,organellefunctions,cellcycle,cytoskeleton,etc.ThesedatasuggestthatbasedonthecytotoxicityofAs2O3,emodinmobilizeeverygenomicresourcethroughwhichtheAs2O3-inducedapoptosisisfacilitated.

简介：为了实现智能家庭服务机器人的人脸跟踪、语音识别、自主移动、动作响应、远程遥控等功能,本智能家庭服务机器人实现了人脸跟踪、语音识别、自主移动、动作响应、远程遥控等功能,家庭服务机器人行走控制

简介：Inthepastdecade,usesofantisepticsanddisinfectantsinhospitalsandotherhealthcarecentersarerathercommon,butthechancetodevelopresistancetoantisepticsanddisinfectantsisalsoincreased.Acinetobacterbaumanniiisoneoftheopportunisticbacteriainvolvinginthenosocomialinfection.Inthepresentstudy,thecorrelationoftheantisepticresistanceinA.baumanniiandtheantisepticresistancegeneqacEΔ1wasinvestigatedbymeansofdeterminationofMICs.Meanwhile,theMICsofglutaraldehyde,chlorhexidine,benzalkoniumbromide,iodophorandtrichloroisocyanurateto80clinicalisolatesofA.baumanniiweredetectedbytubedilutionassayandtheresistancegenesintI1andqacEΔ1intheseisolateswereamplifiedbyPCRandverifiedbyDNAsequencer.ItwasfoundthattheMIC50forthese5antisepticstestedwere32,8,8,4and1μg/mlrespectively,andthedetectionratesofintI1andqacEΔ1genewere60.0%and77.6%respectively.Inaddition,55%ofthe80isolatessimultaneouslypossessedbothintllandqacEΔ1gene,andthepercentageofantisepticresistanceofA.baumanniicarringbothgenestobenzalkoniumbromidewerehigherthanthatwithoutthesetwogenes,however,therewasnosignificantdifferencebetweenintllandqacEΔ1gene.Theresultinbactericidalefficiencyassayindicatedthatchlorhexidinecouldstillproducerapidandstrongbactericidaleffectatconcentrationof1MICafter10minexposure.TheseresultssuggestthattheantisepticresistanceofA.baumanniitovariousantisepticsiscorrelatedwiththepresenceoftheantisepticresistancegenesqacEΔ1inbacteria,thuswarningthattheincreaseoftheantisepticresistanceshouldnotbeignoredandtherelativehighconcentrationorprolongedapplicationtimeisrequiredtoachieveasufficientbactericidaleffect.

简介：Useagenesutureimmersedrecombinanttissue-typeplasminogenactivator(r-tPA)expressionplasmidtotransducemyocardiatopreventthethrombosisaftermechanicaltricuspidvalvereplacementinpigs.MethodsAr-tPAgeneplasmidwasconstructedandconjugatedtoanovelcationicphosphonolipidandar-tPAgenesuturewasmade.Eighteenpigswereselectedanddividedintotwogroupsatrandomization.Therewere9pigsintheexperimentalgroupand9inthecontrolgroup,allthe18pigs'tricuspidswerereplacedwithmechanicalvalves.Thegenethreadsweresuturedintotherightventricularwallsnearmechanicalvalvesandanultrasoundwasusedonthesurfacesoftherightventricularwallsforthegenetransferintheexperimentalgroup.Coagulativefunction,D-dimerlevelofthebloodandthethrombosisonthesurfacesofthevalveswereobserved.Resultsr-tPAgeneplasmidwassuccessfullyconstructedandr-tPAproteinwasexpressedintheventricularcellsaroundthegenesutures.D-dimerreacheditspeaklevel(1.67±0.79)μg·mL-1in1weekafteroperationintwogroups,butitdecreasedtopreoperationlevelthereafterincontrolgroupandkeptonthehighlevelandreincreasedtoanewhighlevel(1.89±0.79)μg·mL-1untiltheendoftheexperimentinexperimentalgroup.Thethrombosesaroundthevalveswerefoundinallthecontrolgroup(100%)butonly1(11.11%)caseinexperimentalgroup.Therewerenochangesinprothrombintimepreandpostoperationintwogroups.ConclusionsUsinggenesutureimmersedr-tPAexpressionplasmidtotransducemyocardiamightbeabestsubstitutionforlifelonganti-coagulationtherapyforthepatients,whounderwentoperation.

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简介：Ecdysteroid荷尔蒙20-hydroxyecdysone在昆虫postembryonic开发和复制起基本作用。几细胞色素P450mono-oxygenases(CYP)，由万圣节前夕基因编码了，被记录了在代表性的昆虫涉及ecdysteroidogenesis在双翅目，鳞翅目和直翅目。这里通常认为的万圣节前夕基因幽灵(Phm，cyp306a1)从hemipteran昆虫种，小棕色的planthopperLaodelphaxstriatellus，被克隆。LsPHM证明五只昆虫保存了P450主题，也就是说Helix-C，螺旋--我，Helix-K，PERF和heme有约束力的主题。LsPhm的时间、空间的表示模式被量的聚合酶链反应评估。通过第四中间形态并且早第五中间形态的阶段，LsPhm在白天2显示出二座表示山峰并且天4−5在白天1和3第四中间形态和天的第四中间形态的美女，和三马槽1第五中间形态。在白天5第四中间形态的美女上，LsPhm清楚地在prothoracic腺被定位的胸有高抄本水平。在美女阶段的LsPhm的双strandedRNA(dsRNA)的饮食的介绍成功地将目标基因击倒，蜕皮激素的减少的表示水平受体(LsEcR)基因并且引起了更高nymph的死亡率并且推迟了开发。LsPhm-dsRNA-exposed美女上的20-hydroxyecdysone的摄取没增加LsPhm表达式水平，但是几乎完全救LsEcRmRNA水平，并且在幸存和开发上减轻否定效果。因此，我们的数据建议通常认为的LsPhm编码在L催化ecdysteroids的生合成的功能的25-hydroxylase。striatellus。