简介:Triglyceride-richhpoprotein(TRL)remnantshavebeenimphcatedbybothclinicalandlaboratorystudiesinthepathogenesisofatheroselerosisandthrombosis.Weuseannewimmunoseparationmethod,providedbyJapanImmunoresearchLaboratories,todeterminetheremnant-hkeparticles(RLP)ofhpeproteins.IsolationofRLPfromserumisachievedusinganimmunoaffmitygelcontainingspecificanti-humanapoA-IandapoB-100(JI-H)monoelonalantibodies,wheretheformerrecognizesHDLandnewlysecretedapoA-I-containingchylomicrons,thelatterrecognizesLDLandmajorityofVLDL.AsaresultofthespecificityoftheJI-Hantibody,themajorityoftheapoB-48-containingchylomicrons,andcertainapoE-enrichedapoB-100-containingVLDLaxenotrecognized.TheselipoproteinsarenotcapturedbytheimmunoaffinitygelandarethusisolatedintheunboundRLPfraction,whichispredominantlymadeupofTRLhavingremnant-hkeproperties.ThecomponentsoftheunboundedfractionaxetheremnantsofCMandVLDLs,verifiedbyultracentrifugationandlipoproteinselectrophoresisinagarosegels.WeestablishedaconvenientapproachtomeasureRLP-cholesterolontheHitachi7150biochemicalanalyser.Thewithin-runandrun-to-runimprecision(CV)oftheassaywas2.75%and11.48%respectively.ThelinearoftheRLP-cinourassaywas0-180mg/dl.Wechose30normallipidslevelforthecontrolgroup(15male&15female),themedianfastingserumRLP-cconcentrationwas8.12±4.88mg/dl.WefredthatRLP-cconcentrationweresignficantlycorrelatedwithTG,VLDL-c,HDL-c&apoE(r=0.765,0.511,-0.378,0.241respectively,P<0.01);andnosignficantcorrelationwassubsequentlyobservedbetweenRLP-candLDL-c.Inourstudy,themedianfastingRLP-cconcentrationweresignificantlyhigherin91patientswithcoronaryarterydisease(CAD),in15patientswithnon-insulindependentdiabetesmeUitus(DM),andin45patientsundergoinghemodialysiswithend-stageretinaldisease