简介：聚氯乙烯上的一个预研项目空纤维ultrafiltrationmembrane过程为对待表面水被进行。膜系统在不同常数下面在thedead结束过滤模式被操作渗入流动。结果证明优化操作(transmembrane压力≤0.1MPa，过滤时间≤30min)与水力的清洗(30s)并且化学清洗(30min，吗清洗的化学药品在过滤的16个周期以后被执行)保证相当稳定的流动(1100L/(m~2吗？h？MPa))并且好渗入质量(混浊<0。1NTU)。照原尺寸的植物能被建议与常数的混合策略操作渗入流动模式(transmembrane压力≤0.1MPa)并且constanttransmembrane压力模式。当未加工的水的温度下面成为5℃时，一个constanttransmembrane压力模式应该被使用；否则一个常数渗入流动模式(transmembranepressure≤0.1MPa)能被操作。这样，极端过滤膜的不可逆的犯规能被最小化保留稳定的流动并且使膜的生活更长。

简介：Fourtestingmethodsusingcationexchangemembrane(CEM),ammoniumacetate,ASI(0.25molL^-1NaHCO3+0.01molL^-1EDTA+0.01molL^-1NH4F)and1.0molL^-1boilingnitricacid,respectively,wereusedtoevaluatesoilavailableK.ThesoilKtestedbyCEMwassignificantlycorrelatedwiththatbytheother(conventional)methods(r^2=0.43^**-0.95^***).ThesoilKtestedbyCEMsaturatedwithNH4HCO3(15minextraction)wasmostcloselycorrelatedwiththatbytheothermethods(r^2=0.60^**-0.95^***),Potassiumavailability,aspredictedbysoiltest,wascomparabletoactualKuptakebycanolaandwheatgrownonthesoilsingrowthchamber.RegressionanalysesshowedthatplantKuptakewasmorecloselycorrelatedwihtKextractedbyCEM(r^2=0.56^**-0.81^***)thanthatbytheconventionalmethods(r^2=0.46^***-0.81^***),mostcolselycorrelatedwiththatbyNH4HCO3-saturatedCEMfor15min(r^2=0.81^***).andworstcorrelatedwiththatbyHNO3(r^2=0.45^**-0.72^***)

简介：Tocloneandconstructtherecombinantplasmidcontainingthemajoroutermembraneprotein(MOMP)geneofChlamydiatrachomatis(C.trachomatis)andtoexpressthefusionproteininE.coliBL21,theMOMPgenewasamphfiedbypolymerasechainreaction(PCR)fromgenomeofC.trachomatisserovarD.ThefragmentwasclonedintotheprokaryoticexpressionvectorpET-22b(+)afterdigestionwithBamHⅠandNotⅠandtransformedintoE.coliXL1-Blue.RecombinantswereselectedbyenzymedigestionandsequencingandtherecombinantplasmidwithMOMPgenewasthentransformedintoE.coliBL21withIPTGtoexpressthetargetgene.TheexpressionrecombinantproteinswerepurifiedbyNi-NTAaffinitychromatography,andidentifiedbySDS-PAGEandWesternblot.Itwasfoundthata1.2kbMOMPgenewasisolated.TheDNAsequenceofMOMPwasfoundtobejustthesameasthesequencepublishedbyGenBank.ArecombinantplasmidcontainingMOMPgenewasconstructedtoexpressthefusionproteinsinE.coli.SDS-PAGEanalysisshowedthattherelativemolecularweightoftherecombinantproteinwasabout47kDathatwasconsistentwiththetheoreticalpredictedvalue,andthespecificityoftheexpressedproteinwasconformedbyWesternblot.ItconcludedthattheMOMPgenecouldbeexpressedintheprokaryoticsystem,bywhichitprovidedthefoundationforthefuturestudiesonthebiologicalactivitiesofC.trachomatisandforthedevelopmentofvaccineagainstthispathogen.

简介：ThispaperdealswiththetransportpropertiesofoligomerofpolythioetherPSAusedasmoblecarrierinbulkliquidmembraneforgold(Ⅲ).ItwasfoundthatAu(Ⅲ)canbetransportedbyPSAfromsourcephasetoreceivingphasecompletelyunderappropriateconditionsandonlyAu(Ⅲ)canbetransferredthroughtheliquidmembranetoreceivingphasefromamixtureofNa(I)-Cu(Ⅱ）-Au(Ⅲ）-Fe(Ⅲ）-Pt(Ⅳ）inthefollowingsystem:HAuCl4-HCl(aq.)/PSA-ClCH2-CH2Cl/(NH2)2CS-HCl(aq.).ThetransportrateofAu(Ⅲ）dependedontheconcentrationofcarrier,thethicknessofliquidmembrane,theconcentrationofAu(Ⅲ）insourcephaseandtheacidityofthemedia.

简介：以便调查在里面聚氨酯(PU)膜由特级的丝绸丝心蛋白粉末(SFP)的加入修改了的一篇小说的vitrobiocompatibility，为小直径的脉管的接枝作好准备，PU和SFP与9:1的比率被混合，7:3，5:5，3:7(PU:SFP)使四成为合成材料。未修改的PU和polytetrafluoroethylene(PTFE)作为控制组被增加。CCK-8试金被用来评估这些biomaterials的cytotoxicity。数据用社会科学统计套装软体，和P被处理<0.05被认为统计上重要。坚持并且在标本的表面上HUVEC传播用直接接触耕作被观察。新奇composites的毒性级别是等级01，它在可接受的范围。在除了控制的所有试验性的组，有1:9的比率的SFP/PU有SFP的最少的cytotoxicity性质，和更多的内容在合成没显示出biocompatibility的改进。强烈属于并且在biomaterials的表面上成长了，并且很快增殖了的HUVEC。增长能力与SFP的增加的比例增加了；当SFP的比例等于时，然而，材料的表面上的房间数量减少了或比PU在的大合成。这新奇材料没有cytotoxicity有优秀细胞的亲密关系到HUVEC，这被结束。增加的SFP给PU更好的biocompatibility，当关于最佳混合比率的进一步的研究仍然被需要时。

简介：Objective:Thepresentstudyaimedtoevaluatethepossibilityofusingcoherentanti-StokesRamanspectroscopy（CARS）microscopytodeterminethespecificmolecularmorphologyofcholesteatomabydetectingthenaturalvibrationalcontrastofthechemicalbondswithoutanystaining.Materialsandmethods:SpecimensfromthemastoidandtympanicmembranewithandwithoutcholesteatomawereanalyzedusingCARSmicroscopy,two-photonexcitedfluorescence（TPEF）microscopy,andthesecondharmonicgeneration（SHG）microscopy.Results:Incholesteatomatissuesfromthemastoid,astrongresonantsignalat2845cm-1wasobservedbyCARS,whichindicatedthedetectionoftheCH2hydro-carbonlipidbondsthatdonotgeneratevisiblesignalsat2940cm-1suggestiveofCH3bondsinaminoacids.Astrongresonantsignalat2940cm-1appearedinanareaofthesamespecimen,whichalsogeneratedabundantsignalsbyTPEFandSHGmicroscopyat817nm,whichwassuggestiveofcollagen.Inthetympanicmembranespecimenwithcholesteatoma,astrongresonantsignalwithcorrugatedmorphologywasdetected,whichindicatedthepresenceoflipids.AstrongsignalwasdetectedinthetympanicmembranewithchronicotitismediausingTPEF/SHGat817nm,whichindicatedcollagenenrichment.TheCARSandTPEF/SHGimageswereinaccordancewiththehistologyresults.Conclusion:TheseresultssuggesttheneedtodevelopanovelCARSmicroendoscopethatcanbeusedincombinationwithTPEF/SHGtodistinguishcholesteatomafrominflammatorytissues.

简介：AIMTo把主要、周期性的pterygium外科的长期的结果与三种不同技术作比较：联合结膜自体移植和覆盖羊膜的膜移植(有AMT的猫)，结膜自体移植移植(猫)独自一个、羊膜的膜移植(AMT)alone.METHODSIn这回顾的研究，142个pterygium病人的142只眼睛(104主要，38周期性)经历了猫(组A)，AMT(组B)或有AMT(组C)的猫分别地后面的外科的切除基于复发和手术后的complications.RESULTSThe数字被考察并且比较一样的描述在下面)，18(10，8)并且2(1，1)在组A，B，和C分别地；干燥眼睛是22(16，6)，27(18，9)并且7(3，4)；结膜发炎是30(17，13)，27(16，11)并且11(6，5)。在组C(主要或周期性的任何一个或两个)的病人主要在组A或B比那些显示出显著地更好的结果(P<0.05)关于上述临床的effects.CONCLUSIONCombined猫并且过分AMT比猫或AMT为主要、周期性的pterygium外科有复发和手术后的复杂并发症的显著地更低的率独自一个。

简介：ObjectiveTheaimofthisstudyistoinvestigatetheeffectoftransfectedhTERTgeneoncellapoptosisofnewbornratcochlearbasilarmembranecells(CBMCs).MethodsCBMCsisolatedfromnewbornratcochlearweretransfectedusingaplasmidcontaininghumantelomerasereverasetranscriptasegene(pCI-neo-hTERT),andwerescreenedusingG418toobtainstabletransfectedcelllines.Cellapoptosisratewasanalyzedbyflowcytometry.hTERTandapoptosisrelatedgenesexpressionweredetectedbyreversetranscriptasepolymerasechainreaction(RT-PCR).ResultshTERTgeneexpressionwasdetected72hoursaftergenetransfectionintransfectedcells.TheapoptoticrateoftransfectedCBMCssignificantlyreduced.Expressionofapoptosisrelatedgenescorrespondinglychanged.ConclusionTransfectionofhTERTgeneleadstoreducedapoptosisrateinnewbornratCBMCs.andlowerexpressionofapaf1,Caspase3andBCL2intransfectedcellsascomparedtothatofnormalCBMCs.

简介：Theinsectmidgutepitheliumiscomposedofcolumnar,goblet,andregenerativecells.Columnarepithelialcellsarethemostabundantandhavemembraneprotrusionsthatformthebrushbordermembrane(BBM)ontheirapicalside.Theseincreasesurfaceareaavailableforthetransportofnutrients,butalsoprovideopportunitiesforinteractionwithxenobioticssuchaspathogens,toxinsandhostplantallelochemicals.RecentimprovementsinproteomicandbioinfbrmaticstoolsprovidedanopportunitytodeterminetheproteomeoftheT.niBBMinunprecedenteddetail.ThisstudyreportstheidentificationofproteinsfromBBMvesicles(BBMVs)usingsingledimensionpolyacrylamidegelelec?trophoresiscoupledwithmulti-dimensionalproteinidentificationtechnology.Morethan3000proteinswereassociatedwiththeBBMVofwhich697werepredictedtopossesseitherasignalpeptide,atleastonetransmembranedomainoraGPI-anchorsignal.Ofthese,bioinfbrmaticsanalysisandmanualcurationpredictedthat185maybeassociatedwiththeBBMVorepithelialcellplasmamembrane.Thesearediscussedwithrespecttotheirpredictedfunctions,namelydigestion,nutrientuptake,cellsignaling,development,cell-cellinteractions,andotherfunctions.Webelievethistobethemostdetailedproteomicanalysisofthelepidopteranmidgutepitheliummembranetodate,whichwillprovideinformationtobetterunderstandthebiochemical,physiologicalandpathologicalprocessestakingplaceinthelarvalmidgut.

简介：ＴＨＥＥＦＦＥＣＴＯＦＲＥＴＩＮＯＩＣＡＣＩＤＯＮＣＥＬＬＭＥＭＢＲＡＮＥＡＮＤＭＥＴＡＳＴＡＴＩＣＡＢＩＬＩＴＹＯＦＭＯＵＳＥＦＯＲＥＳＴＯＭＡＣＨＣＡＲＣＩＮＯＭＡＣＥＬＬＹｕＸｉａｏｄａｎ于晓；ＺｈａｏＸｕｅｍｅｉ赵雪梅；ＧａｏＪｉｎ高进（Ｉｎ...

简介：Inthisstudy,apoly(etherblockamide)(Pebax1657)compositemembraneappliedforCO2capturewaspreparedbycoatingPebax1657solutiononpolyacrylonitrile(PAN)ultrafiltrationmembrane.Ethanol/watermixturewasusedasthesolventofPebaxandtheeffectsofethanol/watermassratiosandPebaxconcentrationonthepermeationpropertiesofcompositemembranewerestudied.Toenhancethecompositemembranepermeance,thegutterlayer,madefromreactiveaminosiliconecrosslinkingwithpolydimethylsiloxane(PDMS),wasdesigned.Theinfluenceofcrosslinkingdegreeofthegutterlayeronmembraneperformancewasinvestigated.Asaresult,aPebax/aminoPDMS/PANmultilayermembranewithhexaneresistancewasdeveloped,showingCO2permeanceof350GPUandCO2/N2selectivityover50.Theblendofpolyethyleneglycoldimethylether(PEG-DME)withPebaxascoatingmaterialwasstudiedtofurtherimprovethemembraneperformance.AfterbeingcombinedwithPEG-DMEadditive,CO2permeanceofthefinalPebax-PEG-DME/amino-PDMS/PANcompositemembranereached400GPUabovewithCO2/N2selectivityover65.

简介：无

简介：Objective:InfraredTympanicThermometer(ITT)isoneofthemostusefulinstrumentsforaccuratelymeasuringtemperature.TheeffectsofearpathologiesonITTmeasurementremainunclear.Thepurposeofthisstudyistodetermineiftympanicmembraneperforation(TMP)affectsITTmeasurementsinadultpatients.Materialandmethods:Atotalof90adultpatientswithmonauralcentralTMPwereenrolledinthisstudy.Patientswerecategorizedintothreesubgroupsaccordingtoperforationsize(1-3mm,4-7mm,and8-10mm).Thetympanictemperaturesoftheaffectedandunaffectedsites,andsubgroupswerecomparedwitheachother.Results:Thisstudycontained54(60%)malesand36(40%)femalesrangingfrom20to58yearsofage(meanage:30.74±9.61years).ThemeantympanictemperatureofthesideaffectedwithTMPwas36.34oC±0.61oC.Themeantympanictemperatureoftheunaffectedsidewithhealthyandintacttympanicmembranewas36.33oC±0.6oC.ThePearsoncorrelationscoreforthetympanictemperaturesandthesizeofTMPwas0.22whichwasnotsignificant(r=-0.12).Conclusion:TMPandperforationsizedonotaffectITTmeasurementsinadultpatients.

简介：ＥＸＰＲＥＳＳＩＯＮＯＦＥＢＶＬＡＴＥＮＴＭＥＭＢＲＡＮＥＰＲＯＴＥＩＮＩＮＥＳＯＰＨＡＧＥＡＬＣＡＲＣＩＮＯＭＡＡＮＤＰＡＲＡＣＡＮＣＥＲＯＵＳＭＵＣＯＳＡＷｕＭｉｎｇｙａｏ吴名耀ＬｉａｎｇＹｉｎｇｒｕｉ梁英锐ＷｕＸｉａｎｙｉｎｇ吴贤英Ｄｅｐａｒ...

简介：Severalcrosslinkedprotonexchangemembraneswithhighprotonconductivitiesandlowmethanolpermeabilitycoefficientswereprepared,basedonthesulfonatedpoly[(4-fluorophenoxy)(phenoxy)]phosphazene(SPFPP)andnewlysynthesizedwatersolublesulfonatedpoly(cyclophosphazene)(SPCP)containingclusteredflexiblependantsulfonicacids.ThestructureofSPCPwascharacterizedbyfouriertransforminfraredspectroscopy(FTIR)and~1HNMRspectra.ThemembranesshowedmoderateprotonconductivitiesandmuchlowermethanolpermeabilitycoefficientswhencomparedtoNafion117.Transmissionelectronmicroscopy(TEM)resultsindicatedthewell-definedphaseseparationbetweenthelocallyanddenselysulfonatedunitsandhydrophobicunits,whichinducedefficientprotonconduction.IncomparisonwithSPFPPmembrane,theprotonconductivities,oxidativestabilitiesandmechanicalpropertiesofcrosslinkedmembranesremarkablywereimproved.TheselectivityvaluesofallthecrosslinkedmembraneswerealsomuchhigherthanthatofNafion117(0.74×10~5S·s/cm~3).TheseresultssuggestedthatthecSPFPP/SPCPmembraneswerepromisingcandidatematerialsforprotonexchangemembraneindirectmethanolfuelcells.

简介：

简介：Nafion-stabilizedPtnanoparticlecolloidalsolutionissynthesizedthroughethyleneglycolreduction.Pt/Nafionaddedwithcarbonblackaselectricconductionmaterial(labeledPt/Nafion-XC72)showsexcellentelectrochemicalpropertycomparedwithPt/C.Aftera300-cycledischargingdurabilitytest,thecellperformanceofmembraneelectrodeassembly(MEA)withthePt/Nafion-XC72andPt/Ccatalystsindicatesa29.9%and92.2%decrease,respectively.ThechargetransferresistancesofPt/Nafion-XC72andPt/Cincreaseby27.2%and101.9%,respectively.TheremainingelectrochemicallyactivesurfaceareaofPtisabout61.7%inPt/Nafion-XC72andabout38.1%inPt/Cafterthedurabilitytest.TheparticlesizeofPt/Cincreasesfromabout5.1nmtoabout10.8nmbutonlyfrom3.6nmto5.8nminthecaseofPt/Nafion-XC72.ThesedatasuggestthatPt/Nafion-XC72asacatalystcanenhancethedurabilityofPEMFCscomparedwithPt/C.

简介：Microporouspolyamidemembrane(Nylon6,PA)wasmodifiedbyaddhydrolysisandthencoupledwithhydroxyethylcellulose(HEC)toamplifyreactivegroupsandreducenonspecificinteractionwithproteins.1,6-Diaminohexane(DAH)asspacearmandphenylalanine(Phe)asligandwereimmobilizedontonylonmembranethroughtheactivationofs-triazinetrichloride(CyCl3).Theaffinitymembraneobtainedcanbeusedtoadsorbγ-globulinspecifically.Theoptimumconditionsofpreparingaffinitymembraneandadsorbingbovineγ-globulinwerestudied.

简介：Flowbatterieswithhighenergydensityandlongcyclelifehavebeenpursuedtoadvancetheprogressofenergystorageandgridapplication.Non-aqueousbatterieswithwidevoltagewindowsrepresentapromisingtechnologywithoutthelimitationofwaterelectrolysis,buttheysufferfromlowelectrolyteconcentrationandunsatisfactorybatteryperformance.Here,anon-aqueouslithiumbrominerechargeablebatteryisproposed,whichisbasedonBr2/Br-andLi+/Liasactiveredoxpairs,withfastredoxkineticsandgoodstability.TheLi/Brbatterycombinestheadvantagesofhighoutputvoltage（3.1V）,electrolyteconcentration（3.0mol/L）,maximumpowerdensity（29.1mW/cm2）andpracticalenergydensity（232.6Wh/kg）.Additionally,thebatterydisplaysacolumbicefficiency（CE）of90.0%,avoltageefficiency（VE）of88.0%andanenergyefficiency（EE）of80.0%at1.0mA/cm2aftercontinuouslyrunningformorethan1000cycles,whichisbyfarthelongestcyclelifereportedfornon-aqueousflowbatteries.

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