简介：Triptolideisanatural,biologicallyactivecomponentderivedfromChineseherbTripterygiumWilfordiiHookF.(TWHF)whichiseffectiveintheclinicaltreatmentofautoimmunediseases,however,themechanismsbywhichtriptolideexertsimmunosuppressionremainfullyunderstood.Theprimaryofthisstudyistodemonstratewhethertriptolidecanaffectphenotype,cytokineproductionandallogeneicTcell-stimulatorycapacityofdendriticcells(DCs)whicharecriticalintheinductionofimmuneresponseortolerance.PhenotypicanalysisshowthattriptolidedoesnotaffecttheexpressionofMHC(Ia^b),CD80,CD86andCD40ofDCstimulatedwithornotLPS,butsignificantlyinhibitsIL12p70productionbyDCinadose-dependentmanner.Triptolide-treatedDCsexhibitareducedcapacitytostimulateproliferationofallogeneicCD4^+Tlymphocytes.Therefore,triptolide-mediatedimmunosuppressionmaydue,inpart,totheinhibitionofIL-12p70productionandimpairmentofallogeneicTcell-stimulatorycapacityofDCs.Ourresultsmayprovideapossiblemechanisticexplanationfortheeffectivenessoftriptolideinthetreatmentofautoimmunediseases.

简介：TheaimofthisstudyistoexplorepotentialpathogenicityofMycoplasmapenetrans,andtoinvestigatewhetherM.penetranslipid-associatedmembraneproteins(LAMPs)couldinducehumanmonocyticcellline(THP-1)toproducesomeproinflammatorycytokinesinvitro,includinginterleukin-1β(IL-1β),tumornecrosisfactoralpha(TNF-α),andIL-8.THP-1wasstimulatedwithdifferentconcentrationsofM.penetransLAMPsandatdifferenttimetoanalyzetheproductionofhumanIL-1β,TNF-αandIL-8.TheproteinlevelsofhumanIL-1β,TNF-αandIL-8weremeasuredbyenzyme-linkedimmunoadsorbentassay(ELISA)andthemRNAlevelsoftheseproinflamrnatorycytokinesweredetectedbyreversetranscriptase-PCR(RT-PCR).ItwasdemonstratedinthepresentstudythattheproductionofIL-1β,TNF-αandIL-8increasedindose-andtime-dependentmannerafterstimulationwithM.penetransLAMPsinTHP-1cells.M.penetransLAMPsalsoinducedtheexpressionofIL-1β,TNF-αandIL-8mRNA.TheproductionofIL-1β,TNF-αandIL-8andtheexpressionofmRNAweredown-regulatedbypyrrolidinedithiocarbamate(PDTC).ThisstudydemonstratedthatM.penetransLAMPscaninducetheproductionofproinflammatorycytokinesinhumanmonocyticcellsinvitro,thussuggestingthatitmaybeanimportantetiologicalfactor.

简介：TostudythegeneticcharacterizationoffourstrainsofBorreliaburgdorferiisolatedinChina.PCRtechniquewasusedtoamplifythe5S-23SrRNAintergenicspacerDNAfromthewholecellularDNAofisolatedGXLD-4,9,18andChang14,andthentheamplifiedproductswereclonedintoplasmidpGEM-TEasyandsequenced.Itwasfoundthatthe5S-23SrRNAintergenicspacerDNAofthefourisolateswas242bp,revealingthenucleotidesequenceidentityofmorethan99%.ThefourisolateshadhighersequenceidentifywithBorreliavalaisianathanwithothergeneticgroups.ThesefourisolatesmostlikelybelongtoBorreliavalaisianagenomicgroup.

简介：【摘要】 目的 分析6s管理在物质管理中的应用价值。方法 抽选来自2017年4月至2018年4月期间我院未实施6s管理的物质管理作为对照组，将2018年5月至2019年5月期间我院采用实施6s管理的物质管理作为观察组 ，比较两组出现物品存放不合理例数、寻找物品消耗时间、清洁环境耗时、月盘点耗时。结果 观察组物品存放不合理例数显著低于对照组，差异具有统计学意义，P＜0.05；观察组寻找物品消耗时间、清洁环境耗时、月盘点耗时显著短于对照组，差异具有统计学意义，P＜0.05。结论 对物质管理中采用6s管理，能够避免不良事件，有助于物品核对，减少物品存放不合理例数，缩短寻找物品消耗时间、清洁环境耗时、月盘点耗时间，在临床上显示出卓越成效，值得进一步推广使用。

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简介：Toinvestigatetheprevalenceandgenotypeofextendedspectrumbeta-lactamases(ESBLs)mediatedbyplasmidinGram-negativebacteriafoundinsouthernChina,atotalof1184clinicalisolatesofnon-repetitivestrainsofGram-negativebacteriawerecollectedin2001from5differentcitiesinsouthernChina.TheESBLs-producingisolatesweredistinguishedbymeansofthephenotypeconfimatorytestbasedontheNCCLScriteriaandweresubjectedtoplasmidconjugationandelectroporationexperiments.Thoseclinicalisolatessucceededinplasmidtransfershadundergoneplasmidconjugationandelectro-transformation,plasmidDNAextractionandPstⅠdigestlinger-printinganalysis,aswellasthetmiversalprimerPCRamplificationoftheTEM,SHV,CTX-M,VEB,PERandSFOgenesandtheDNAsequencinginordertodeterminethegenotypesofESBLsandtheirplasmidlocations.ItwasfoundthattheincidenceoftheESBLs-producingstrainsofGram-negativebacteriawas14.6%(173/1184)with67strainsoftransconjugantsand11strainsofelectro-transformants,inwhichCTX-M-14typewas33.3%(26/78);CTX-M-3typewas23.1%(18/78);CTX-M-9typewas14.1%(11/78);CTX-M-5typewas6.4%(5/78);CTX-M-13typewas2.6%(2/78);SHV-5typewas7.7%(6/78);SHV-12typewas5.1%(4/78),SHV-2atypewas2.6%(2/78)andunidentifiedtypewas5.1%(4/78).29.5%ofthewildstrainsalsocarriedbroad-spectrumbeta-lactamasesTEM-1andSHV-1types.TheabovementionedESBLsgeneswerelocatedontransferableplasmidswithvariablesizes(from35to190kb).TheCTX-MtypeESBLswascharacterizedbyhigh-levelofresistancetocefotaxime.ItconcludedthattheCTX-M-typewasthemostprevalentgenotypeinclinicalisolatesofGram-negativebacteriainsouthernChina,andtheSHVtyperanksinthesecondplace.TEM-,VEB-,Toho-andPER-typeswerenotfoundintheseisolates.

简介：Tosupportthescientificbasisforrapididentificationofpathogenicbacteriaandotherstud-ies,thesequencesofhsp60geneinmajor34speciesof16genusofpathogenicbacteriaweresearchoutinGenBankandaproperpairofuniversaldegenerateprimerwasdesignedbymeansofthemolecu-larbiologicalsoftwawePrimer5.0andOligo6.0.ThisprimerwasthenusedinthePCRamplification,andthehsp60genefragmentsoftheselectedpathogenicbacteriacouldbeamplifiedusingthisdegener-ateprimer.Bywayofbioinformationalanalysis,theconservation,variationandtheinterspeciesphylo-geneticrelationsofthehsp60genesequencewereanalysed.Fromtheresultsofthecomparativestudyonsequences,itwasdemonstratedthatthehsp60genewascharacterizedbyconservationandvaria-tion,inwhichtheconservedandmutantregionsco-existedandseparatelydistributedwithmanysmallmutantregionsdistributedamongtheconservedregions,justlikethemosaic.Thephylogenetictreeamongdifferentpathogenicbacteriadrawnfromthehsp60geneanalysiswasprovedtobeconsistentwiththosefrom16SrRNAand23SrRNA.Itisconcludedthatthesequencedistributionofhsp60genewouldprovideasolidbasisfortherapididentificationofpathogenicbacteriaandthedevelopmentofadiagnosticmicroarray.

简介：During2004,atotalof124batchesofHIVantibodyELISAsfromdomesticandoverseasmanufacturers,comprisingapproximately60milliontests,weretestedforqualityandreleasedforscreeningbloodinChina.Theinter-andintra-batchvariation,specificity,andsensitivitywereevaluatedusingalaboratorypanelandclinicalsamples.Theinter-batchvariationwaslessthan15%andonly2of12assayshadintra-batchvariationoflessthan20%for4dilutionsofacontrolspecimen.257samplesconfirmedpositiveforHIVantibodyand4826negativesamplesfromdifferentregionsinChinawereusedtoevaluatethesensitivityandspecificityoftheassays.Theresultsshowedthatthesensitivityisintherangefrom93.7%to100%forassayssampleddirectlyfromthemanufacturers,and91.4%-99.6%forthoseretrievedfromtheconsumers;thespecificitywasintherangefrom97.88%to99.97%.ThetestingenvironmentmayvaryindifferentregionsofChina.Therefore,manufacturersshouldproviderobustassaystosatisfytherequirementsofthesediverseenvironments,andespeciallyreducetheintra-assayvariationandimprovethestabilityofthekits.

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简介：摘要：近年来，越来越多的医院开始采用生产管理模式，从早期的全面质量管理到现在公认的管理6S实质性管理是有效的实施。6S管理，用于医院管理和道德建设，以创建清洁、安全、人的尊严、规范和尊重的理想医院的文化和道德，提高医院管理的科学知识和精细度，从而提高医院的竞争力。

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简介：摘要：目的 对 6S管理在护理技术强化训练中的应用效果予以探究。方法 选取本院 2014级护生 253名在实训教学中予以常规管理作为对照组， 2015级护生 230名在实训教学中予以 6S管理作为观察组。对两组护生护理技术实训考核成绩、教学满意度、综合素质自我评定优良情况进行对比。结果 观察组护生护理技术实训考核成绩高于对照组（ P<0.05）；观察组护生教学满意度 83.48%，高于对照组的 55.34%（ P<0.05）；除人际沟通能力与语言表达能力外，观察组护生其他项目综合素质自我评价优良例数均高于对照组（ P<0.05）；上述差异均有统计学意义。结论 6S管理在护理技术强化训练中的应用效果显著。

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简介：Wereportedanovelmammalianreovirus,designedBYD1,isolatedfromthroatswabsofpatientswithsevereacuterespiratorysyndrome(SARS),in2003.Inthepresentstudy,wefirstlycomparedthegenomeelectrophoreticmigrationpatternsofreovirusBYD1with3prototypereovirusstrainsbypolyacrylamidegelelectrophoresis(PAGE)anddeterminedthecompletenucleotidesequenceoftheS1genesegmentofBYD1bysingleprimeramplificationtechnique.TheelectropherogramofBYD1wasdifferentfromthoseofthe3prototypestrainsandanyotherreovirusisolatesreportedbefore.TheentireS1segmentsequenceofBYD1is1437bplongwithtwomeaningfulopenreadingframes(ORFs).ThelongestORFencodesσ1,thecellattachmentprotein,andthesecondlongestORFsupposedlyencodesσ1s,animportantnonstructuralvirulencefactor.TheterminalsequencesofS1segmentare5'GCUAand3'UCAUC,whichareconsistentwiththoseofothermammalianreoviruses.Thehighesthomologyofdeducedσ1aminoacidsequenceis64%identitywithknownmammalianreoviruses.PhylogeneticanalysisofbothS1nucleotidesequenceandσ1aminoacidsequenceindicatedtheBYD1isolatebelongedtoanewcladeofserotype2group.TheresultsofthisstudyshowedthattheBYD1S1segmentwasmarkedlydifferentfromthoseofisolatesreportedbeforeandBYD1wasanovelhumanreovirusisolate.

简介：ThisstudywasaimedtoobservetheexpressionofP70S6kinase(P70S6K)inoralaciniccellcarcinoma.PT0S6kinaseexpressionwasexaminedbymeansofWestern-blottestandActivityas-say.Specimenswerefrom30casesoforalaciniccellcarcinomaand15casesofnormaloraltissuewereusedascontrols.StatisticalanalysissoftwareSPSS10.0wasusedforttesttodeterminetherelationshipbetweengeneexpressionandclinicalfeatures.TheexpressionlevelofP70S6Kincreasedobviouslyinoralaciniccellcarcinomatissue(P<0.01).ActivityassaywasthesameastheWestemblottest(P<0.01).P70S6Kexpressionlevelandactivityplayedanimportantroleinthedevelopmentoforalaciniccellcarcinoma.Inconclusion,P70S6Kisamplifiedandoverexpressedinoralaciniccellcarci-nomatissue,whichsuggestsapotentialoncogenicfunction.P70S6KandotherpossibletargetsofmTORcontributesignificantlytotumordevelopmentandthatinhibitionoftheseproteinsmaybethera-peuticforcancerpatients.OverexpressionofP70S6Kmaybeinvolvedinthepathogenesisoforalacin-iccellcarcinoma.

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简介：摘要：目的：对 6S活动的开展对提高住院环境满意度的影响。方法：通过 6S活动的前后开展对比，选取我科室 2017年 10到 12月的 100名患者（对照组）和 2018年 5到 7月的 100名患者（观察组），根据 11个选项结合自身感受进行病房环境舒适度的评分。结果 观察组患者选项 1、 2、 3、 4、 5、 9、 10、 11项差异明显（ P﹤0.05），即通过活动的开展，提高了住院环境的满意度。结论 6S活动的开展，对住院患者满意度的影响较大，能提高住院患者的满意度。

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简介：【摘要】 目的： 研究 6S 管理法在手术室护理管理 中的运用效果 。 方法： 选取 2018 年 3 月 -2019 年 8 月期间，我院接收的 72 例手术室患者 ， 利用 随机数字表法，将其分为实验组和参照组，每组 36 例；参照组 采用手术室常规护理管理法，实验组采用 6S 管理法，对比两组效果 。 结果： 比较后显示，实验组患者对护理质量的评分更高，满意度上升，与参照组相比 （ P<0.05 ） ，统计学具有意义。 结论： 在手术室护理管理中，实施 6S 管理法，可以提升护理质量，取得让患者满意的结果 。