简介：Poly-α-aminoacidisakindofbiodegradablebiomedicalmaterial.Itisusedforthepreparationofsustaineddrug-releasedevice.Therearesomeadvantagesofthedrugdeliverydevices.Theycanprovidethetherapywithsideeffects.Thedrugre-leaseismaintainedatabeneficialrateforaprolongedtime.Thepolymermaybebiodegradedafterthedrughasbeenexhausted.

简介：ThepurposeofthisstudywastodesignabioreactorforbioarLificialliverandstudyitsefficacythroughinvitroexperiment;testifiedthesafetyandefficacyoftheBALthroughtreatmentwithBALonacutefulminantischemichepaticfailurecaninemodels.

简介：Ｔ－ＬＹＭＰＨＯＣＹＴＥＭＥＤＩＡＴＥＤＴＵＭＯＲＣＥＬＬＤＥＳＴＲＵＣＴＩＯＮＩＮＶＩＶＯＡＳＳＯＣＩＡＴＩＮＧＷＩＴＨＡＳＰＥＣＩＦＩＣＦＥＡＴＵＲＥ　ＯＦ　ＡＰＯＰＴＯＳＩＳＹｕＤａ鱼达；ＹａｎｇＨｕａ杨骅；ＺｈｅｎｇＳｈｕ郑树；ＷａｎｇＸｉａ...

简介：Thefeasibilityoffenestrationoperationinmiddleearbonewithpulsedinfraredlaserisevaluated.HealthymaleNewZealandrabbitsinvivoareusedintheexperiment.Middleearmastoidboneofanimalmodeliscompletelyexposedwithconventionalmethods,andthenapulsedCO2laser（10.6μm）andanEr:YAGlaser（2.94μm）areusedtoperformthefenestrationoperation.Diamonddrillisalsousedasacontrolgroup.Thetotaloperationtimeandlightirradiationtimearerecordedandtheopeningefficiencyisassessed.Themorphologicalchangesandthermaldamagearoundtheopeningwindowonthemiddleearboneareexamined.Itisshownthatbothlasersystemsaresuitableforthefenestrationoperationinmiddleearbone,andthisno-touchtechniquehasalotofbenefitscomparedwithtraditionalmethods.Thebleedingduringoperationhasanimportanteffectonoperationtimeandthermalinjuryandneedstobecontrolledefficientlyinfurtherstudy.更多还原

简介：干细胞治疗旁边在医疗保持大诺言，例如，代替失去的房间，重新组成健康房间人口并且另外在干细胞的使用作为为因素和基因交货的车辆。胚胎的干细胞正直地由于他们在vivo.Tissue特定的干细胞在胚胎区分进任何房间类型的证明能力吸引了大兴趣是不管多么已经在在诊所的使用，并且最近包含人的神经干细胞(NSC)的第一系统的医药试用治疗被发射了。Thereare还克服的许多障碍和过程到改善。保证进步在医药我们文件结束干细胞增加了管理干细胞特征的分子的机制的基本知识是必要的。这里，我们在房间治疗的各种各样的方面在NSC上提供文学的评论，与使用简历材料控制NSCcharacteristics，区别，和交货的潜力的主要焦点。我们在啮齿类动物模型在内长、移植的NSC的特征上从研究总结结果神经病学并且癌症疾病，和热点在聚合物相容性的最近的前进和处于regulatingNSC状态和命运的适用性。我们建议特殊设计的聚合物的发展，如此的ashydrogels，是一个关键问题在中枢神经系统改进干细胞治疗的结果。

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简介：AbstractBackground:Angiogenesis and hypoxia-inducible factor 1α (HIF-1α) play major roles in solid tumors. This study aimed to establish a longitudinal and multimodal imaging model for in vivo evaluation of HIF1α and angiogenesis in breast cancer.Methods:By transfection of a 5 hypoxia-responsive element (HRE)/green fluorescent protein (GFP) plasmid, the cell line Ca761-hregfp was established, which emitted green fluorescence triggered by HIF-1α under hypoxia. The cells were subjected to CoCl2-simulated hypoxia to confirm the imaging strategy. We grew Ca761-hre-gfp cells in the left rear flanks of twelve 615 mice. Experiments were conducted on days 4, 9, 15, and 19. For in vivo analysis, Ca761-hre-gfp subcutaneous allografted tumors were imaged in vivo using contrast-enhanced ultrasound (CEUS) and fluorescence imaging (FLI) during tumor development. The tumor size, CEUS peak intensity, and FLI photons were measured to evaluate tumor growth, angiogenesis, and HIF-1α activity, respectively. After each experiment, three mice were randomly sacrificed and tumor specimens were collected to examine HIF-1α activity and the microvessel density (MVD).Results:In vitro, both green fluorescence and HIF-1α expression were detected in Ca761-hre-gfp cells treated with CoCl2, indicating the suitability of the cells to detect HIF-1α activity. In vivo, HIF-1α activity first increased and then decreased, which was significantly correlated with angiogenic changes (r = 0.803, P = 0.005). These changes were confirmed by immunohistochemical staining of HIF-1α and MVD.Conclusions:The findings validated the Ca761-hre-gfp murine allograft model for reliable evaluation of HIF-1α activity and angiogenesis longitudinally using both molecular and pre-clinical non-invasive imaging modalities. The cell line may be useful for studies of anti-HIF pathway therapies.

简介：2月12日,vivo通过微博宣布正式成立新品牌iQOO,透过该品牌vivo全面冲击5000元以上的价位旗舰手机。据了解,此前vivo从未针对旗下的任何一个产品单独开设官方微博,这次开设iQOO足见重视。

简介：试图学习在里面vivo男白化体老鼠上的Tabernaemontanadivaricata叶摘录的反煽动性的活动。方法水的煎和甲醇叶摘录为他们减少巴豆的能力被测试在在热门申请以后的鼠标耳朵的导致油的浮肿。甲醇叶摘录dose-dependently禁止了巴豆在老鼠的导致油的耳朵浮肿(ID50<500?g

简介：Theanticoagulanteffectofleechsalivawastraditionallyemployedinthetreatmentofdiabetesmellituscomplicationssuchasperipheralvascularcomplications.Thisstudywascarriedouttoexaminetheeffectofleechsalivaextract(LSE)onbloodglucoselevelsinalloxan-induceddiabeticrats.First,LSEwascollectedfromleecheswhichwerefedonaphagostimulatorysolution.Second,totalproteinconcentrationwasestimatedusingtheBradfordassay.Third,diabeticratswereinjectedsubcutaneously(sc)withLSEatdosesof500and1000μg·kg1bodyweight(bw).Otherdiabeticratswereinjectedscwithinsulinatdosesof10and20U·kg1bw.AnothergroupwasinjectedsimultaneouslywithLSE(250μg·kg1bw)andinsulin(10U·kg1bw).Fastingbloodglucose(FBG)concentrationsweremonitoredduringastudyperiodofeighthoursatregularintervals.FindingsshowedthatbothdosesofLSEresultedinasignificantandgradualdecreaseinFBGstartingfrom10%18%downfallaftertwohoursofinjectionreachingthemaximalreductionactivityof58%aftereighthours.Remarkably,LSEwassufficienttobringtheratstoanearnorm-glycemicstate.Thehighdoseofinsulininducedaseverehypoglycemicconditionafter24hofinjection.ThelowerdosewasabletodeclineFBGfor26hinratswhichbecamediabeticagainafter8h.Ontheotherhand,theconcurrentinjectionoflowdosesofLSEandinsulinproducedahypoglycemiceffectwithallratsshowingnormalFBGlevels.Takentogether,thesefindingsindicatedthatthesubcutaneousinjectionofLSEofthemedicinalMalaysianleechwasabletoprovidebetterglycemiccontrolcomparedwithinsulin.Moreover,thesynergismbetweenLSEandinsulinsuggeststhatLSEcouldbeutilizedasanadjuvantmedicationinordertoreduceinsulindosageortoachievebettercontrolofbloodglucose.

简介：Thepaperdescribesdrugreleaseevaluationofabiodegradablelong-actingcon-traceptiveCapsule(CaproF)containinglevonorgestrel(LNG)invivo.Poly(E-Capro-lactone)(PCL)biodegradablematerial,suitableformanymedicalapplication,wasusedinthisstudy.Itwasextrudedintotubes.ThetubeswerethencutandloadedwithLNGpowderbeforemelting-sealedonbothendstogetdrugcapsules.Thissub-dernalimplanthasbeenfoundtobehighlyeffectiveinanimalexperiments.Twoad-

简介：TheaimofthepresentstudywastodeterminetheefficacyofimmunotherapywithdendriticcellstoelicitEBV-specificCTL-immunityinadvancedcasesofEBV-positivepatientswithnasopharyngealcarcinoma(NPC)andtodeterminethesafetyandtoxicityofthispreparation.NinecasesofhistologicallyconfirmedpatientswithNPCundergoingtreatmentwithradiologicaltherapywereenrolledinthisstudy.Dendriticcells,generatedinvitrofrombloodmonocytesofpatientswereculturedandmaturedwithcytokinesandtheninfectedwithrecombinantadenovirusvaccinecontainingEBV-latentmembraneprotein-2(Ad-LMP2).On9days'cultivationofcells,thematuredDCswereharvested,irradiatedwithCoandtheninjectedintradermallytopatientswithNPC.Theinjectionswereperformed3timestotally.Afterimmunization,theCTLresponseswereassayedbymeansofcytotoxicityandepitope-specificIFN-γproduction.Theresultsofthistrialshowedthatallpatientscouldtoleratethiskindoftreatmentwithoutanysideeffect,duringwhichmarkedincreaseofLMP2-specificCTL-responsescouldbedemonstratedin5patientsofthisgroup.AndthelevelofIgA/VCAantibodydecreasedin8of9patients,thusaccountingforabetterprognosisforthesepatients.Allpatientswillbefollowedupforanotheroneyear.Atleast,thepresentworkshowsthatintradermalvaccinationwithautologousDCsinfectedwithrecombinantAd-LMP2adenovirusisasafeprocedureinNPCpatients,inwhichthisprocedurecanenhancetheLMP2-specificCTLresponsesinpatients.Thesedataareencouragingtodevelopmoreeffectivevaccinestrategiesforthetreatmentofnasopharyngealcarcinoma.

简介：Vivo于8月2713在北京新中关举行了新品X5的线下活动会。此次体验会别出心裁的将体验部分放到了一辆被改造成KTV房样式的大巴上举行，大巴上的“KTV设备”就是Vivox5，也突出了vivoX5主打“KTV”功能的特点。被称为“K歌神器”的X5配备了高级独立Hi—Fi方案：定制版CS4398音频解码芯片，德州仪器高级输入芯片TLV320ADC以及AB类耳放MAX97220的，并且针对KTV功能首次在手机上内置了顶级YAMAHA数字环绕声信号处理芯片YSS205X—CZE2。此款芯片被业内誉为“卡拉OK界传奇芯片”，能够真实还原高档KTV包房的效果。

简介：近日，VIVO宣布在巴西的SaoPaulo地区和RiodeJaneiro地区推出3GCDMA20001xEV—DO网络，设备由Lucent提供。该网络可以为便携式电脑和配备了1x—EV—

简介：BackgroundCoronarymicroembolization(CME)ischaracterizedbydistalmicrovascularocclusion.However,theinflammatorymechanismsandtherapeutictargetsofCMEarelargelyunknown.MethodsAtotalof11GuangxiBamaminiatureswinesweredividedintotwogroups:sham(n=5)andCME(n=6).MicrosphereswereinjectedintotheleftanteriordescendingarteryoftheCMEgrouptomakeananimalmodelofCME.TheexpressionsofmicroRNA-146a(miR-146a)andIRAK1,TRAF6,andAUF1inthemyocardiumweredetectedbyqPCR.ResultsIntheCMEgroup,microspheres,microinfarction,andinflammatorycellinfiltrationwerefoundunderanopticalmicroscope.TheexpressionlevelsofmiR-146awerelowinbothgroups.AfterCME,theexpressionlevelsofIRAK1,TRAF6,andAUF1intheCMEgroupwereupregulatedcomparedwiththoseintheshamgroup(P<0.01;P<0.05;P<0.05,respectively).ConclusionsAUF1,IRAK1andTRAF6,butnotmiR-146a,couldbeinvolved,inmyocardiuminflammationfollowingCME.

简介：AbstractObjective:The aim of this work was to explore the feasibility of in vivo and non-invasive monitoring of deuterium/hydrogen (2H/1H) exchange at the metabolic level upon exposure to heavy water (2H2O).Methods:The healthy female mice were randomly assigned to two groups after day 0 when both mice received standard drinking water. The treated mouse was fed with 2H2O (80%, v/v) and the control mouse fed with standard drinking water (H2O) over next 13 days. Real-time mass spectrometric analysis of volatile metabolism emitted through breathing and the skin was performed on days 1, 2, 3, 10, 12, and 13. Animal experiment was approved by the Laboratory Animal Ethics Committee of Jinan University (approval No. 20161117163322) on October 29, 2021.Results:We observed a replacement of 1H by 2H in 52 mass spectral features (60 2H/1H isotopologue pairs) for the mouse fed with 2H2O, but not for the control mouse. These included pyruvic acid and lactic acid, lysine and methyl-lysine as well as short-chain fatty acids comprising acetic acid, propionic acid, butyric acid and valeric acid.Conclusion:Secondary electrospray ionization-high resolution mass spectrometry allows monitoring in vivo2H-incorporation of metabolites in a non-invasive and real-time setup and opens new opportunities to use 2H tracing to extend current metabolic studies, especially those with a focus on anaerobic glycolysis, lysine methylation and gut microbiome via monitoring of short-chain fatty acids.

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简介：nVIDIA系列的又一个重量级名字：“钛”。它能给我们带来什么样的惊喜，我们一起来看看。